Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,726,433	Δ11 bp	coding (411‑421/600 nt)	nemR →	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1726433	1726443	11	13 [0]	[0] 13	nemR	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1726432	0 (0.000)	13 (0.540)	13/286	0.8	100%	coding (410/600 nt)	nemR	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
	?	NC_000913_3_hsa_tpiA	1726444 =	0 (0.000)					coding (422/600 nt)	nemR	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

TTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCA  >  NC_000913_3_hsa_tpiA/1726289‑1726545                                                                                                                                                |                                                                                                                  ttaCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAACGTCCTCGCGGCGTGATCGCCCTGCTtgcaa                                                                                                              >  2:58076‑M1/1‑144 (MQ=255)     cAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatg                                                                                                          <  2:118594‑M1/149‑10 (MQ=255)                    cAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccatt                                                                                           >  1:181056‑M1/1‑125 (MQ=255)                                        cATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatagccgtgagaaccattgtttaaccttttgtggcgaa                                                                       <  2:367957‑M1/149‑45 (MQ=255)                                                       gACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAACGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttaaccttttgtggcgaaccgctgcaacaggcc                                                        >  1:118402‑M1/1‑90 (MQ=255)                                                        acacgaaaactctcCGCCGATGTGTGCGATCTGTCAGAAGTCATCGGCCGCGCTTTGGATAAAGGTTCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttaaccttttgtggcgaaccgctgcaacaggcac                                                       <  1:56438‑M1/144‑61 (MQ=255)                                                            tAAAACTCTCTGCCGAAGTGTGCGAGCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttaaccttttgtggcgaaccgctgcaacaggcacaagt                                                   <  2:207948‑M1/149‑65 (MQ=255)                                                                 ctctctGCCGAAGGGTGCGATCTGTCAGAAGATATGCGGAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttatccttttgtggcgaaccgctgcaacaggcacaagtgcttt                                              <  2:249491‑M1/149‑70 (MQ=255)                                                                                   gATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttaaccttttgtggcgaaccgctgcaacaggcacaagtgctttacgcactgtggcttggcg                            >  2:74424‑M1/1‑62 (MQ=255)                                                                                           agaagaTATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttaaccttttgtggcgaaccgctgcaacaggcacaagtgctttacgcactgtggcttggcgcgaatctg                    >  1:302487‑M1/1‑54 (MQ=255)                                                                                                             gATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttaaccttttgtggcgaaccgctgcaacaggcacaagtgctttacgcactgtggcttggcgcgaattaacagaccaacatttcgcgc  >  2:328152‑M1/1‑36 (MQ=255)                                                                                                                 gATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttaaccttttgtggcgaaccgctgcaacaggcacaagtgctttacgca                                         >  1:348667‑M1/1‑32 (MQ=255)                                                                                                                 gATAAAGGTGCTCGCGGCGTGATCGCCCTGCTtggaaaatggccgtgagaaccattgtttaaccttttgtggcgaaccgctgcaacaggcacaagtgctttacgca                                         <  2:348667‑M1/106‑75 (MQ=255)                                                                                                                                                |                                                                                                                  TTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTTTAACCTTTTGTGGCGAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCA  >  NC_000913_3_hsa_tpiA/1726289‑1726545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑