Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,743,514	C→T	L20F (CTC→TTC)	mdtK →	multidrug efflux system transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,743,514	0	C	T	100.0%	38.3 / NA	12	L20F (CTC→TTC)	mdtK	multidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/8);  total (4/8)

TTTTTGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTG  >  NC_000913_3_hsa_tpiA/1743394‑1743648                                                                                                                         |                                                                                                                                       tttttGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATggg                                                                                                            >  1:219039/1‑149 (MQ=255)              ttCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATtttc                                                                                                                                      >  1:39504/1‑110 (MQ=255)              ttCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATtttc                                                                                                                                      <  2:39504/110‑1 (MQ=255)                            tAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGgcgggcgg                                                                                 >  2:356420/1‑149 (MQ=255)                             aGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGggcggc                                                                                <  2:342419/149‑1 (MQ=255)                              ggctctcATATAACTAAAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCt                                                                               <  2:25387/147‑1 (MQ=255)                               tctctcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCta                                                                              <  2:219039/149‑1 (MQ=255)                                ctctcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtat                                                                             <  1:356420/149‑1 (MQ=255)                                              cAAATAAAAGGTGTTCAGGTGCAGAAGTATATCGGTTGGGTGGGTCTTTTAGTAGCATTAGAAATCGGGGTGGTTTTCGCGCAAATTGCCCAAACTGCGATGAGATTTGTCGAAACCGTGATGGCGGCCGGCTATAGTGCCACCGACAt                                                               >  1:12407/1‑149 (MQ=255)                                                    aaaGGTGTTCGCGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcggc                                                         <  1:50023/149‑1 (MQ=255)                                                          gtTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcggcactata                                                   <  2:227243/149‑7 (MQ=255)                                                                                gTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCtt                             <  1:189023/149‑1 (MQ=255)                                                                                                    gcattagcaATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGCCTTCCGGCGCTCCTCTCTGGTcc         >  1:284298/1‑148 (MQ=255)                                                                                                           caATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGActg  <  1:294519/149‑1 (MQ=255)                                                                                                                         |                                                                                                                                       TTTTTGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTG  >  NC_000913_3_hsa_tpiA/1743394‑1743648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑