Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	2,977,097	Δ1 bp	coding (499/1032 nt)	galR →	galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	2,977,096	0	G	.	100.0%	372.0 / NA	76	coding (498/1032 nt)	galR	galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (38/38);  total (38/38)

CTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGA  >  NC_000913_3_hsa_tpiA/2976948‑2977241                                                                                                                                                     |                                                                                                                                                  ctgcGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCctggctgc                                                                                                                                                  >  3:206549/1‑149 (MQ=255)  tgcGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGCTCGTTACGGTGCctggctgca                                                                                                                                                 <  2:101898/150‑3 (MQ=255)   gcGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCctggctgcaa                                                                                                                                                >  2:489990/1‑147 (MQ=255)         gtcgtcCATGCCAAAATGTTCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTc                                                                                                                                         <  1:803321/150‑1 (MQ=255)         gtcgtcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTc                                                                                                                                         <  1:229001/150‑1 (MQ=255)          tcgtcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCa                                                                                                                                        <  3:462909/150‑1 (MQ=255)            gtcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGt                                                                                                                                          >  4:47505/1‑146 (MQ=255)            gtcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGt                                                                                                                                          <  3:47505/146‑1 (MQ=255)            gtcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCAtt                                                                                                                                      >  3:287859/1‑150 (MQ=255)               cgtGCCAAAATGGTGCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTTTCCTGCCTGGCTTTGAATACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTaa                                                                                                                                   <  4:224840/148‑1 (MQ=255)               cATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTaa                                                                                                                                   <  1:793671/150‑1 (MQ=255)                aTGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAAt                                                                                                                                  >  1:471771/1‑150 (MQ=255)                 tGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAAtt                                                                                                                                 <  3:479541/150‑1 (MQ=255)                   ccAAAATGATCCCGGATGCTGATTTAGCCTCAGTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTCGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCa                                                                                                                               <  4:38887/150‑1 (MQ=255)                    cAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAAcgc                                                                                                                                            >  2:137510/1‑136 (MQ=255)                    cAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAAcgc                                                                                                                                            <  1:137510/136‑1 (MQ=255)                    cAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAg                                                                                                                              >  2:463808/1‑150 (MQ=255)                            tCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCa                                                                                                                      >  4:143267/1‑150 (MQ=255)                            tCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCa                                                                                                                      >  3:180657/1‑150 (MQ=255)                               cGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATAc                                                                                                                   <  2:442902/150‑1 (MQ=255)                                ggATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATAcc                                                                                                                  <  3:356535/150‑1 (MQ=255)                                  aTGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCg                                                                                                                <  2:771621/150‑1 (MQ=255)                                    gCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCa                                                                                                              >  1:241122/1‑150 (MQ=255)                                      tGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATAccc                                                                                                                 <  1:31889/145‑1 (MQ=255)                                      tGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATAccc                                                                                                                 >  2:31889/1‑145 (MQ=255)                                              ccTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCt                                                                                                    <  2:450736/150‑1 (MQ=255)                                              ccTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGGTATCt                                                                                                    <  4:439440/150‑1 (MQ=255)                                                tCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCtgt                                                                                                  >  4:203296/1‑150 (MQ=255)                                                  aTTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAg                                                                                                                          <  2:647631/124‑1 (MQ=255)                                                  aTTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAg                                                                                                                          >  1:647631/1‑124 (MQ=255)                                                  aTTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGc                                                                                                <  1:774610/150‑1 (MQ=255)                                                     aaTGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGctct                                                                                             >  1:68321/1‑150 (MQ=255)                                                        gAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAAc                                                                                          <  1:510963/150‑1 (MQ=255)                                                        gAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAAc                                                                                          >  2:368453/1‑150 (MQ=255)                                                        gAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAAc                                                                                          >  2:405885/1‑150 (MQ=255)                                                          aaCAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCa                                                                                        >  3:358346/1‑150 (MQ=255)                                                           aCAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCAc                                                                                       >  1:706035/1‑150 (MQ=255)                                                            cAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCAct                                                                                      <  2:471771/150‑1 (MQ=255)                                                             aaaTGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCActc                                                                                     <  1:489990/150‑1 (MQ=255)                                                             aaaTGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCActc                                                                                     >  4:527198/1‑150 (MQ=255)                                                                    cGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCt                                                                              <  2:241122/150‑1 (MQ=255)                                                                      gTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGa                                                                            >  3:116873/1‑150 (MQ=255)                                                                          ggTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGcc                                                                        >  1:278377/1‑150 (MQ=255)                                                                           gTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCg                                                                       >  4:251767/1‑150 (MQ=255)                                                                             gCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCctggctg                                                                                                                                                   <  2:738953/73‑1 (MQ=255)                                                                             gCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCctggctg                                                                                                                                                   >  1:738953/1‑73 (MQ=255)                                                                             gCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGaa                                                                     >  1:553206/1‑150 (MQ=255)                                                                                 aTCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATc                                                                 <  3:359147/150‑1 (MQ=255)                                                                                 aTCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATc                                                                 <  4:97689/150‑1 (MQ=255)                                                                                 aTCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATc                                                                 >  2:708811/1‑150 (MQ=255)                                                                                   cAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGt                                                               >  1:420582/1‑150 (MQ=255)                                                                                   cAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGt                                                               >  2:588478/1‑150 (MQ=255)                                                                                   cAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCACTTACTTCAGACACCACCTACCAGAACTGGTTCTCTGTGCTCTAACCACTCTATTTCTGACGCCGCAGATCGt                                                               >  1:296389/1‑150 (MQ=255)                                                                                     aCCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTcc                                                             >  2:427575/1‑149 (MQ=255)                                                                                     aCCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCt                                                             <  1:463808/150‑1 (MQ=255)                                                                                               gcctgGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTAt                                                   >  1:112746/1‑150 (MQ=255)                                                                                               gcctgGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTAt                                                   >  1:80529/1‑150 (MQ=255)                                                                                                cctgGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTAtt                                                  <  2:823525/150‑1 (MQ=255)                                                                                                        tGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGcc                                          <  4:180657/150‑1 (MQ=255)                                                                                                         gAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGccc                                         <  2:50123/150‑1 (MQ=255)                                                                                                         gAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGccc                                         >  2:165939/1‑150 (MQ=255)                                                                                                          aaaaCCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTg                                                                                                           <  3:292168/83‑1 (MQ=255)                                                                                                          aaaaCCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTg                                                                                                           >  4:292168/1‑83 (MQ=255)                                                                                                              ccGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCt                                    <  3:527198/150‑1 (MQ=255)                                                                                                                   gTATTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAg                               >  3:376113/1‑150 (MQ=255)                                                                                                                     aTTGCTCTGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTg                             >  1:679656/1‑150 (MQ=255)                                                                                                                     aTTGCTCTGGACGATCGTTACGGTGCCGGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATGTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTg                             <  2:296389/150‑1 (MQ=255)                                                                                                                         ctctGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGa                                                                            >  4:121982/1‑99 (MQ=255)                                                                                                                         ctctGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGa                                                                            <  3:121982/99‑1 (MQ=255)                                                                                                                          tctGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTAtt                        >  1:137140/1‑150 (MQ=255)                                                                                                                           ctGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCTGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTg                       >  2:143621/1‑150 (MQ=255)                                                                                                                            tGGACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGc                      <  2:420582/150‑1 (MQ=255)                                                                                                                             ggACGATCGTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCg                     <  3:12891/150‑1 (MQ=255)                                                                                                                             ggACGATCGTTACAGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCg                     <  3:282337/150‑1 (MQ=255)                                                                                                                                     gTTACGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGa             >  4:147679/1‑150 (MQ=255)                                                                                                                                         cGGTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCgg         <  4:348433/150‑1 (MQ=255)                                                                                                                                           gTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCg          <  1:586585/147‑1 (MQ=255)                                                                                                                                           gTGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCg          >  2:586585/1‑147 (MQ=255)                                                                                                                                            tGCCTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTg      >  3:262030/1‑150 (MQ=255)                                                                                                                                             gCCTGGCT‑GCAACGCGTAATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTgg     <  2:385941/150‑1 (MQ=255)                                                                                                                                              ccTGGCT‑GCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGt    <  3:203296/150‑1 (MQ=255)                                                                                                                                                tggct‑gcaACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGa  <  1:368453/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGA  >  NC_000913_3_hsa_tpiA/2976948‑2977241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑