Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	3,946,711	C→A	noncoding (12/120 nt)	rrfC →	5S ribosomal RNA of rrnC operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	3,946,711	0	C	A	100.0%	88.4 / NA	42	noncoding (12/120 nt)	rrfC	5S ribosomal RNA of rrnC operon
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/18);  total (24/18)

TGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAAGCAGTAAGCCG  >  NC_000913_3_hsa_tpiA/3946569‑3946838                                                                                                                                               |                                                                                                                                tGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAgcgc                                                                                                                          <  1:730876/150‑1 (MQ=17)  gAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGcgcg                                                                                                                         >  2:13261/1‑150 (MQ=17)  gAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGcgcg                                                                                                                         <  3:285939/150‑1 (MQ=17)   aGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCgg                                                                                                                        >  2:491408/1‑150 (MQ=17)      tAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCggtgg                                                                                                                     >  2:307181/1‑150 (MQ=17)        aCCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTc                                                                                                                   >  1:378790/1‑150 (MQ=17)        aCCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTc                                                                                                                   <  1:757612/150‑1 (MQ=17)          cGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTccc                                                                                                                 >  2:119696/1‑150 (MQ=17)                 aaTGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGAc                                                                                                          >  4:310586/1‑150 (MQ=17)                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGAcc                                                                                                         >  2:231190/1‑150 (MQ=17)                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGAcc                                                                                                         >  3:42279/1‑150 (MQ=17)                     aaCCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCa                                                                                                      >  2:733235/1‑150 (MQ=17)                       ccGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATg                                                                                                    <  2:413251/150‑1 (MQ=17)                       ccGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATg                                                                                                    >  4:338659/1‑150 (MQ=18)                       ccGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATCTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATg                                                                                                    <  4:112056/150‑1 (MQ=17)                         gTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGcc                                                                                                  <  1:607403/150‑1 (MQ=17)                         gTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATAAAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGcc                                                                                                  >  1:298272/1‑150 (MQ=17)                                ttAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCa                                                                                           <  2:631718/150‑1 (MQ=17)                                ttAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCa                                                                                           <  4:430753/150‑1 (MQ=17)                                ttAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCa                                                                                           <  1:561425/150‑1 (MQ=17)                                  aaCCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGa                                                                                         >  1:246281/1‑150 (MQ=17)                                   cccTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGaa                                                                                        <  1:827977/149‑1 (MQ=17)                                   aCCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGaa                                                                                        <  3:191215/150‑1 (MQ=17)                                   aCCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGaa                                                                                        <  3:191080/150‑1 (MQ=17)                                      ttACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGTCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTg                                                                                     <  1:72408/150‑1 (MQ=14)                                       tACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGa                                                                                    >  2:380225/1‑150 (MQ=17)                                        aCAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGaa                                                                                   <  2:740036/150‑1 (MQ=17)                                         cAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGaaa                                                                                  >  2:818904/1‑150 (MQ=17)                                          aaCGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAgc                                                                                                                            >  3:165800/1‑107 (MQ=18)                                          aaCGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAgc                                                                                                                            <  4:165800/107‑1 (MQ=18)                                          aaCGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAAc                                                                                 >  2:33365/1‑150 (MQ=17)                                          aaCGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAAc                                                                                 >  2:6192/1‑150 (MQ=17)                                          aaCGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAAc                                                                                 >  2:583670/1‑150 (MQ=17)                                          aaCGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAAc                                                                                 >  1:189852/1‑150 (MQ=17)                                                   gATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAgcg                                                                        >  1:195080/1‑150 (MQ=11)                                                    aTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAgcgc                                                                       >  1:169192/1‑150 (MQ=11)                                                                                                              aGCGGTCTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTaa             <  2:220300/150‑1 (MQ=11)                                                                                                                  gTCTGAGGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAAGCAg         >  2:431702/1‑150 (MQ=17)                                                                                                                    cTGATGAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAAGCAGTa       >  4:390410/1‑150 (MQ=17)                                                                                                                     tgatgaAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAAGCAGTaa      >  1:702520/1‑150 (MQ=17)                                                                                                                        tgaAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAAGCAGTAAGcc   <  3:338659/150‑1 (MQ=14)                                                                                                                         gaAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGAGAGAGTTTGGAACTGCCAGGCATCAAATTAAGCAGTAAGCCg  <  1:201199/150‑1 (MQ=2)                                                                                                                                               |                                                                                                                                TGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAAGCAGTAAGCCG  >  NC_000913_3_hsa_tpiA/3946569‑3946838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑