Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1978503	1979278	776	35 [0]	[1] 36	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1978502	0 (0.000)	33 (0.960)	29/260	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_hsa_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

CCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTA  >  NC_000913_3_hsa_tpiA/1979139‑1979420                                                                                                                                             |                                                                                                                                              gtctttaccattatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCa                                                                                                                                       <  2:568581‑M2/9‑1 (MQ=255)  tctttaaacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCAt                                                                                                                                      <  1:486189‑M2/10‑1 (MQ=255)    tttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacccgcacatacaacggaggggggctgcgaatttcaataatgcgtgatgcagatcacacaaaaaaatcaattacttaaaataaattTATAATTCATAc                                                                                                                                    >  2:207032‑M2/138‑149 (MQ=255)    tttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaaaactcaattacttaacataaatgTATAAGTCATAc                                                                                                                                    >  2:673166‑M2/138‑149 (MQ=255)         acttatctaagatttttttttttttttcgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcatatcacacaaaactctcaattacttaacataaatgTATAAGTCATActtttt                                                                                                                               >  2:393341‑M2/133‑148 (MQ=255)                taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacccaaaacactcaattacttaacataaatgTATAAGTCATAATTTTGTTTtggt                                                                                                                        >  2:334650‑M2/126‑148 (MQ=255)                taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcacttacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                                                                        >  1:477439‑M2/126‑149 (MQ=255)                taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacagacaacggaggggggatgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTCTAAGTCATACTTTTGTTTTggg                                                                                                                        >  1:607526‑M2/126‑149 (MQ=255)                 aagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatccaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTTTTTTGGgt                                                                                                                       >  1:498590‑M2/125‑149 (MQ=255)                   gatttttcctaaatcaacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgtgt                                                                                                                     <  1:111495‑M2/27‑1 (MQ=255)                     tttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaatatcttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTAt                                                                                                                   >  1:472574‑M2/121‑149 (MQ=255)                        ttcctaaatcgacgcaactgtactcgtcactacacgcacctccaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCCTACTTTTGTTTTGGGTGTATTTc                                                                                                                >  2:307724‑M2/118‑149 (MQ=255)                           ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggatgggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                             >  1:163286‑M2/115‑149 (MQ=255)                            taaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATc                                                                                                            >  2:510839‑M2/114‑149 (MQ=255)                            taaagcggcgcaaatgtgcttgttactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGTGTGTATTTCAATc                                                                                                            <  2:307002‑M2/36‑1 (MQ=255)                                               tcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactaacttacttagcatatatgTATAAGTCATACTTTTGTTTTGGGTGTCTTTCAATCTGTTAAAAACTTTTTcgct                                                                                         >  2:76350‑M2/95‑149 (MQ=255)                                                 gtcactacacgcacatacaacagaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTTGGTGTATTTCAATCTGTTAAAAAGTTTTTccctac                                                                                       >  2:307170‑M2/93‑149 (MQ=255)                                                         acgctcatacgacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaa                                                                               <  1:374971‑M2/65‑1 (MQ=255)                                                                tacaacggaggggggctgcgatttgcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGAGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaaa                                                                        <  2:368594‑M2/72‑1 (MQ=255)                                                                     cggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaaa                                                                   <  2:346203‑M2/77‑1 (MQ=255)                                                                           ggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaa                                                             >  2:658294‑M2/67‑149 (MQ=255)                                                                                    tgttttaatgatgcgtgatgcagatcacacaaaacacgcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGaaa                                                    <  1:334650‑M2/92‑1 (MQ=255)                                                                                        tcaagaatgggtgaggcagatgacccaaaactgtctattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTACATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATggg                                                <  2:410725‑M2/96‑1 (MQ=255)                                                                                         caataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGa                                               <  1:547777‑M2/97‑1 (MQ=255)                                                                                                            tcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATCGGATGTTGCCCCCATTCAACAt                            >  2:652236‑M2/34‑149 (MQ=255)                                                                                                             cacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATa                           >  1:354865‑M2/33‑149 (MQ=255)                                                                                                             cacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATa                           >  1:354775‑M2/33‑149 (MQ=255)                                                                                                              acataaaactcttaattacttaacacaaatgTATAAGTCATACTTTTGCTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATaa                          <  1:170155‑M2/118‑1 (MQ=255)                                                                                                               cacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGAGACCAGTCAACATAAc                         >  2:578161‑M2/31‑149 (MQ=255)                                                                                                                  aaaacactctattacttaacttaaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCa                      <  2:159504‑M2/122‑1 (MQ=255)                                                                                                                    aaccctcaattacttaacatagatgTATAAGGCATAGTTTTGTTTTGGGGGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCAcc                    <  1:395754‑M2/124‑1 (MQ=255)                                                                                                                     acactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCg                   >  1:228775‑M2/25‑149 (MQ=255)                                                                                                                            aatactaaacataaatgTATAAGTCATACTTTTGTGTTGGGTTTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAaataa            <  2:269518‑M2/132‑1 (MQ=255)                                                                                                                                cttaacataaatgTATAAGTCATACTTTTGTTTGGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGATTAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAatcat        <  1:449902‑M2/136‑1 (MQ=255)                                                                                                                                 tgaacataaatgGATTAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCGCCGTAAATAatcgtc       <  2:478455‑M2/137‑1 (MQ=255)                                                                                                                                      aTAAATGTATAAGTCATACTTTTGTACTGGGTGTATTTCAAACTGTTAAAAAGTTTTTCGCTACGATAGCAAGCAAAGATCAAATAGGAATAAATGAAATACGATGTTGCGCACAGACATAATCACCGACCATAAATAatcagacccaa  >  2:433760/1‑142 (MQ=255)                                                                                                                                             |                                                                                                                                              CCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTA  >  NC_000913_3_hsa_tpiA/1979139‑1979420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑