Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 3,793,162 G→T 17.6% F174L (TTC→TTA)  yibB ← YibB family protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA3,793,1620GT17.6% 70.6 / 2.0 37F174L (TTC→TTA) yibBYibB family protein, function unknown
Reads supporting (aligned to +/- strand):  ref base G (15/14);  new base T (5/2);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATA  >  NC_000913_3_hsa_tpiA/3793018‑3793308
                                                                                                                                                |                                                                                                                                                  
ttgaaattCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTAACAGTAAGCCCTTTTTTTATAGTGaaaa                                                                                                                                                >  2:350049/7‑149 (MQ=255)
   aCTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCTCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGAt                                                                                                                                             <  2:233432/149‑1 (MQ=255)
     tttCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTACAGTGAAAAGATGc                                                                                                                                           >  2:85401/1‑149 (MQ=255)
           gAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTTAAAAGCTGCATCTTa                                                                                                                                     >  2:136425/1‑149 (MQ=255)
             aCTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTACCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACt                                                                                                                                   <  1:535895/149‑1 (MQ=255)
                 aTTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtca                                                                                                                               >  1:230343/1‑149 (MQ=255)
                             cTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTTTAGTGAAAAGATGCATCTTACTTTCATCAAATGGGaaa                                                                                                                   <  2:559069/149‑1 (MQ=255)
                                tCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATcc                                                                                                                >  1:304330/1‑149 (MQ=255)
                                   aTTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTATATAGTGAAAAGATGCATCTTACTTTCATCAAATGCGAAATCCCaa                                                                                                             >  2:517338/1‑149 (MQ=255)
                                    tttATTCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCaaa                                                                                                            <  1:343744/149‑1 (MQ=255)
                                                aCCAACAATAGCGCCCCCAACAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTc                                                                                                <  1:124535/149‑1 (MQ=255)
                                                 ccAACAATTGCGCCGCCAATAATATATACATGGTTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAACATGCATCTTACTTTCCTCACATAGGAAAGACCAACTTTGAAATCCTca                                                                                               >  1:257893/1‑148 (MQ=255)
                                                             cccccAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGTGTTACATTTgg                                                                                   <  1:432102/149‑1 (MQ=255)
                                                               cccAATAATATGTACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCt                                                                                 >  1:430780/1‑149 (MQ=255)
                                                                ccAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTTTTTTTTTTACATGAATATTGCCTTCATCAAATGGGACATCCCAAATTTTAAATCCTCGAGTT‑CAttttatctt                                                                               >  2:261994/1‑143 (MQ=255)
                                                                  aataatATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTAt                                                                              <  2:471433/149‑1 (MQ=255)
                                                                  aataatATATACACAATTGCCAAACATGAAATCAAAAACCTGATGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTTAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTAt                                                                              <  1:70501/149‑1 (MQ=255)
                                                                       tatataCATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACACTAAGCCCTTTTTTTATAGTGAAAAGATGCACCTTAATTTCAACAAATGGAAAATCCAAAAATTTCAATCATCGAGTAAAACTCGGCTTATTGCaa                                                                         >  2:326840/1‑149 (MQ=255)
                                                                            aCATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGcc                                                                    >  1:604566/1‑149 (MQ=255)
                                                                                  ttGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTTTATTTGAAACATGGATCTTACTTTCATCAAATAGGAAATCCTAAATTTTCACTCCCCAAGTTACATTGGTCTTATTGCACTTGCCaaaacc                                                              >  2:121547/1‑147 (MQ=255)
                                                                                       aaTCATGAAATAACAAACCGGACGTGGAGATGTTACAGCAAGCCCTCTATCGGAAGCAAACCGATGCAGCAAGCGCTCATCAAATGGGAAATACCAAATTTTCAATGTTGAAGTTACATTTGGCTTATGGCAATAACCAAAATCTATcc                                                         >  2:209146/1‑149 (MQ=255)
                                                                                         tCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATTTTTTTAAGATGCAACTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGTCAATAGCCAAAATCTATCCAc                                                       >  2:210894/1‑149 (MQ=255)
                                                                                           aTGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTTTTTTTTTTAAATACATCATACTTTAATCAAATGGAAAATCCCAAATTTTCACTCCTCGAGTTACCTTTGGATTTTTCCAATACCCAAAATCTATctcgtt                                                     >  2:457219/1‑144 (MQ=255)
                                                                                               aaTCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCGTCTTACTTACATCAAATGTTAAAGCCCAAATTTTCACTGTGTGAGGTACATTTGTCTTATGGCAATAGCCAAAATTTATCCTCGcaaca                                                 >  2:1047/1‑149 (MQ=255)
                                                                                                 tCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAag                                               >  1:513221/1‑149 (MQ=255)
                                                                                                  cAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCGTCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAaga                                              <  2:450369/149‑1 (MQ=255)
                                                                                                                tgAGATTTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTaa                                <  2:203192/149‑1 (MQ=255)
                                                                                                                 gagaTGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAg                               >  1:560367/1‑149 (MQ=255)
                                                                                                                  agaTGTGCCAGTAAGCCCTTTTTTTATAGTGAACAGATGCATCCTACGTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGc                              <  1:188835/149‑1 (MQ=255)
                                                                                                                  agaTGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGc                              <  2:538065/149‑1 (MQ=255)
                                                                                                                       tgACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATg                         >  1:616293/1‑149 (MQ=255)
                                                                                                                        gACAGTAAGCCCGTTTTTTATAGTGAAAAGATGCATCTTACTTTCATGCAGTTGGAAAACCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGt                        <  1:537905/149‑1 (MQ=255)
                                                                                                                         aCAGTAAGCCCTTTTTTTATAGTTAAACGATGCATCTTACTTTCATCAAATGGGAAATCCTAAATTTTCAATCCTCGAGTTAAATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGtt                       <  1:173988/149‑1 (MQ=255)
                                                                                                                             tAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGTCGTTTTCACTAAGCCCATGTTGATa                   >  1:274587/1‑149 (MQ=255)
                                                                                                                                gCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCt                <  1:470069/149‑1 (MQ=255)
                                                                                                                                    tttttttATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCtttat            >  1:13523/1‑149 (MQ=255)
                                                                                                                                              gTGAAAAGATGCATCTTACTTTCCTCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATa  <  1:321916/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TTGACTTTCCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATA  >  NC_000913_3_hsa_tpiA/3793018‑3793308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑