Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,748,487	A→T	I453F (ATC→TTC)	ydhS →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,748,487	0	A	T	98.9%	316.9 / NA	90	I453F (ATC→TTC)	ydhS	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (46/43);  minor base C (0/1);  total (46/44)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAG  >  NC_000913_3_hsa_tpiA/1748340‑1748630                                                                                                                                                    |                                                                                                                                                gCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTc                                                                                                                                               <  1:944912/150‑1 (MQ=255)  cGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGCCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTcc                                                                                                                                              >  2:707188/1‑150 (MQ=255)    ggAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTGGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCt                                                                                                                                            >  3:592591/1‑150 (MQ=255)        tcatgcatATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTcc                                                                                                                                        <  2:1076345/150‑1 (MQ=255)           ttcatATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTg                                                                                                                                     >  4:691249/1‑150 (MQ=255)           ttcatATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTg                                                                                                                                     <  3:691249/150‑1 (MQ=255)           ttcatATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGATTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTg                                                                                                                                     >  1:886234/1‑150 (MQ=255)             catATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGACAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGCTGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTggg                                                                                                                                   <  4:284548/150‑1 (MQ=255)             catATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTGCTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTggg                                                                                                                                   <  2:736224/150‑1 (MQ=255)             catATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTggg                                                                                                                                   >  4:538062/1‑150 (MQ=255)                  tctcGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATCGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTAcg                                                                                                                              >  4:97292/1‑150 (MQ=255)                         cTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCaa                                                                                                                       <  1:388226/150‑1 (MQ=255)                          tCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAAt                                                                                                                      <  2:898013/150‑1 (MQ=255)                              tGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACa                                                                                                                  <  2:997782/150‑1 (MQ=255)                                     tACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACa                                                                                                           >  1:274278/1‑150 (MQ=255)                                     tACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACa                                                                                                           >  3:627777/1‑150 (MQ=255)                                       cAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAgg                                                                                                         <  1:661327/150‑1 (MQ=255)                                        aaaaTGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAggg                                                                                                        <  4:482428/150‑1 (MQ=255)                                        aaaaTGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAggg                                                                                                        <  3:518193/150‑1 (MQ=255)                                        aaaaTGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAggg                                                                                                        >  4:183091/1‑150 (MQ=255)                                              gAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGaa                                                                                                  >  2:925263/1‑150 (MQ=255)                                                    aaTGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCt                                                                                            <  2:1076899/150‑1 (MQ=255)                                                     aTGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTg                                                                                           >  2:954502/1‑150 (MQ=255)                                                      tGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa                                                                                          <  1:865907/150‑1 (MQ=255)                                                        aGTCGCGCACAGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATg                                                                                        >  1:324201/1‑150 (MQ=255)                                                            gcgcACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTgg                                                                                    >  3:109803/1‑150 (MQ=255)                                                            gcgcACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTgg                                                                                    <  2:274278/150‑1 (MQ=255)                                                              gcACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCg                                                                                  >  4:123601/1‑150 (MQ=255)                                                               cACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGa                                                                                 <  4:109803/150‑1 (MQ=255)                                                                aCCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGaa                                                                                <  3:130618/150‑1 (MQ=255)                                                                  cGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTATTTATTGATGCCCGCGGACAGCGTCTGATTAAAGCGAAGGATCTCCCGCTCCCTGGGCTACGCGCGCAATTACAGAACACAGGGGATGTAATCCCTGATGTTGGCGAAGa                                                                              <  4:3890/150‑1 (MQ=255)                                                                      cTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATtat                                                                          <  3:58500/150‑1 (MQ=255)                                                                      cTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATtat                                                                          <  3:183091/150‑1 (MQ=255)                                                                       tGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATtata                                                                         <  1:531769/150‑1 (MQ=255)                                                                       tGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATtata                                                                         >  4:581940/1‑150 (MQ=255)                                                                        gAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGCTGTTGGCGAAGATTATAc                                                                        >  2:1033246/1‑150 (MQ=255)                                                                         aaaaCGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATTCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACg                                                                       >  2:885715/1‑150 (MQ=255)                                                                            aCGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAACCAGGGGTGTAAATCCCTGCTGTTGGCGAAGAGTATACGTTa                                                                    >  2:337259/1‑150 (MQ=255)                                                                             cGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTAc                                                                   <  3:538062/150‑1 (MQ=255)                                                                             cGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTAc                                                                   >  1:1044628/1‑150 (MQ=255)                                                                              ggAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACa                                                                  <  3:429565/150‑1 (MQ=255)                                                                               gAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAg                                                                 <  3:581940/150‑1 (MQ=255)                                                                                  gACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCaa                                                              >  2:583416/1‑150 (MQ=255)                                                                                   acaacaGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATAc                                                                        >  2:878756/1‑139 (MQ=255)                                                                                   acaacaGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATAc                                                                        <  1:878756/139‑1 (MQ=255)                                                                                     aacaGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAAt                                                                                                <  3:485394/113‑1 (MQ=255)                                                                                     aacaGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAAt                                                                                                >  4:485394/1‑113 (MQ=255)                                                                                     aacaGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATtata                                                                         <  1:925738/136‑1 (MQ=255)                                                                                     aacaGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATtata                                                                         >  2:925738/1‑136 (MQ=255)                                                                                     aacaGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAAccc                                                           >  1:111525/1‑150 (MQ=255)                                                                                       caGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGa                                                         >  4:333781/1‑150 (MQ=255)                                                                                        aGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGaa                                                        >  3:284541/1‑150 (MQ=255)                                                                                          cTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATtata                                                                         <  2:348897/131‑1 (MQ=255)                                                                                          cTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATtata                                                                         >  1:348897/1‑131 (MQ=255)                                                                                              tCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCACTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATAtt                                                  >  3:552375/1‑150 (MQ=255)                                                                                                gTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCg                                                >  2:684153/1‑150 (MQ=255)                                                                                                   tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTgg                                             <  2:776722/150‑1 (MQ=255)                                                                                                     aCGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGgc                                           >  1:136326/1‑150 (MQ=255)                                                                                                       gTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGgcgc                                         >  2:175741/1‑150 (MQ=255)                                                                                                         ttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGt                                       <  1:954502/150‑1 (MQ=255)                                                                                                          tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTa                                      <  1:684153/150‑1 (MQ=255)                                                                                                          tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTa                                      >  1:925128/1‑150 (MQ=255)                                                                                                               tGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGtt                                 >  2:157962/1‑150 (MQ=255)                                                                                                                gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAggg                                                                                                        <  2:956911/78‑1 (MQ=255)                                                                                                                gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAggg                                                                                                        >  1:956911/1‑78 (MQ=255)                                                                                                                   gCCCGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGgc                             <  4:432194/150‑1 (MQ=255)                                                                                                                     ccGCGGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCAGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATACGGGGGCGCGTAGCGTTCGgcgc                           >  3:189216/1‑150 (MQ=255)                                                                                                                      cgcgGACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACATAAAACAGGGGATGAATTCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGcgcg                          <  1:707188/150‑1 (MQ=255)                                                                                                                         ggACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTg                       <  4:627777/150‑1 (MQ=255)                                                                                                                          gACAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGc                      >  1:246307/1‑150 (MQ=255)                                                                                                                           aCAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGcc                     >  1:356671/1‑150 (MQ=255)                                                                                                                           aCAACGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGcc                     <  1:412367/150‑1 (MQ=255)                                                                                                                               cGTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTgg                 <  1:102496/150‑1 (MQ=255)                                                                                                                                gTCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGt                >  2:132203/1‑150 (MQ=255)                                                                                                                                 tCCGCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGtt               >  3:145100/1‑150 (MQ=255)                                                                                                                                   cGCTTAAAGTGAAAGATTTCCCTTTCCCTGTGCTACGCGAGCAATTACAGAAAACAGTGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGa             <  1:845955/150‑1 (MQ=255)                                                                                                                                    gCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTgg                                                                                    >  1:141040/1‑78 (MQ=255)                                                                                                                                    gCTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTgg                                                                                    <  2:141040/78‑1 (MQ=255)                                                                                                                                     cTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATg           <  4:592591/150‑1 (MQ=255)                                                                                                                                     cTTAAAGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATg           <  2:204938/150‑1 (MQ=255)                                                                                                                                          aGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGtt               >  1:1020069/1‑141 (MQ=255)                                                                                                                                          aGTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGtt               <  2:1020069/141‑1 (MQ=255)                                                                                                                                           gTGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGAc     <  1:885715/150‑1 (MQ=255)                                                                                                                                            tGAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGAcc    >  1:548316/1‑150 (MQ=255)                                                                                                                                             gAAAGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATAAGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGAccg   >  1:77622/1‑149 (MQ=255)                                                                                                                                              aaaGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACa                                                                                                           <  1:353278/45‑1 (MQ=255)                                                                                                                                              aaaGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACa                                                                                                           >  2:353278/1‑45 (MQ=255)                                                                                                                                              aaaGATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAg  >  1:437518/1‑150 (MQ=255)                                                                                                                                                 gATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGcc                     >  3:288618/1‑128 (MQ=255)                                                                                                                                                 gATTTCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGcc                     <  4:288618/128‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                GCGGGAATCATTCATATTCTCGCCCTCGGTGAAGACTACAAAATGGAAATTAATGAGTCGCGCACCGTCCTGAAAACGGAAGACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGCAACCCGAAGATATTCGTGGGCGCGTAGCGTTCGGCGCGTTGCCCTGGTTGATGCACGACCAG  >  NC_000913_3_hsa_tpiA/1748340‑1748630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑