New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 346876141 (0.920)4 (0.090) 3/270 NT 8.5% coding (1093/2694 nt) chiA periplasmic endochitinase
?NC_000913_3_hsa_tpiA 3469086 = 46 (1.050)coding (768/2694 nt) chiA periplasmic endochitinase

TCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTGGGGCTGGCTGAACATCAGGAGCCGGTTTAA  >  NC_000913_3_hsa_tpiA/3468938‑3469228
                                                                                                                                                    |                                                                                                                                              
tCACAGCACCGCCATTATTATCCGGCTTAACGGAACCGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTTTTTCGCCTCGCCTGGGGCATTTGCCGCGCCt                                                                                                                                                >  1:690543/1‑149 (MQ=39)
tCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCCGGGCAATTTGCCGCGCCt                                                                                                                                                >  2:694088/1‑149 (MQ=255)
          gCCATTATTATCCAGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCCAACATAACGCCAAGAGTTACTGCCATCGATTACTTTCGCATCGCCTGGCAAATTCGCCCACCCTAccccaccga                                                                                                                                      >  2:261815/1‑143 (MQ=255)
           ccATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc                                                                                                                                     <  1:457203/149‑1 (MQ=255)
           ccATTATTATCCGGCTTAACGGAACAGGACGCGACGTTTCCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTCTTTTCGCATCGCCCGGGCAATTTGACGCGGCTACCCACCAGGc                                                                                                                                     >  2:388032/1‑149 (MQ=25)
              acgccgccgttatcaatttcacaggtagtcgggttaccgtactgactcaattccgcagcggtagctgtacgctcgagacgccatgggttattggagttttcagcgctggtaccccgtgggcaatctttagacccCACGCCCCAGGCGtt                                                                                                                                  >  1:315102‑M2/135‑149 (MQ=255)
                    tCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCCAGCGTTCTtata                                                                                                                            >  2:462505/1‑149 (MQ=255)
                    cccGGCTTAATGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCTTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTCTCGCACCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTtata                                                                                                                            <  2:636128/148‑1 (MQ=255)
                                    aGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg                                                                                                            >  1:753091/1‑149 (MQ=255)
                                            gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTa                                                                                                    <  1:455334/149‑1 (MQ=255)
                                             ggTTGCCATACTTTCTGATTTCTGTTGCCGTTGCCGCCCGAACATAACGCCATCGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAc                                                                                                   <  1:694643/149‑1 (MQ=255)
                                             ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAc                                                                                                   >  1:537194/1‑149 (MQ=255)
                                              gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTAGTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAGCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc                                                                                                  >  1:603337/1‑149 (MQ=255)
                                               ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCa                                                                                                 <  1:210736/149‑1 (MQ=255)
                                                      aCTGGCTGATGTCTGTTGGCGTTGCTGGGCGAACAGAACGCCATGGGTTATTGCGATCGTTGTCTTTCGCTCCGCATGGGCAATTTGCCGTGCATACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTagaa                                                                                          <  2:47334/149‑1 (MQ=255)
                                                          gCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAAc                                                                                      >  2:265851/1‑149 (MQ=255)
                                                          gCTGATTTCCGTTGCCGTTGCCGTCGGAACATAACGCCTAGTGTTACTGGCATCGTGTGCTTTCGCATGGTCCGGGCGATTGTCCTTGCCTACCCTGCAGGAGTTCTTATAAATGACGCCGTTATAGATAACGTACCATGTAGAAGAAc                                                                                      <  2:583288/149‑1 (MQ=255)
                                                              aTTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTc                                                                                  >  2:289758/1‑149 (MQ=255)
                                                                   tGTTGCGTTTGCCGCTGGAACCTAACGCCAGGGGTTACTGGCATTGTTTTCTTTCGCATCTCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGAc                                                                             <  2:364846/149‑1 (MQ=255)
                                                                     ttgccgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCt                                                                           <  2:537194/149‑1 (MQ=255)
                                                                       gccgttgccgCGCGAACATAACGCCATGGGATACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAGGTAGAAGAACCTTCCTGACtttt                                                                         >  1:448121/1‑149 (MQ=255)
                                                                        ccgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATGACGTACCAGGTCGAAGAACCTTCCTGGTCTTTc                                                                        >  2:45898/1‑149 (MQ=39)
                                                                         cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTcc                                                                       <  1:333010/149‑1 (MQ=255)
                                                                         cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAACAGCCTTCCTGATCTTTcc                                                                       >  2:40527/1‑149 (MQ=255)
                                                                                gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGctac                                                                >  1:473251/1‑149 (MQ=255)
                                                                                       cATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCa                                                         >  1:231804/1‑149 (MQ=255)
                                                                                        aTAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCAGTTCTAGGTAATGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCAt                                                        >  1:578345/1‑149 (MQ=255)
                                                                                         tatcGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAGTGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATa                                                       <  2:564422/146‑1 (MQ=255)
                                                                                         tAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGTCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATa                                                       <  1:246728/149‑1 (MQ=255)
                                                                                           aCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGt                                                     >  1:600792/1‑149 (MQ=255)
                                                                                           aCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGt                                                     >  1:60737/1‑149 (MQ=255)
                                                                                            cGCCATGTGTTACGGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGtt                                                    <  1:130573/149‑1 (MQ=255)
                                                                                                    gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAAGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTCCAGCATAGTTGTCGttgg                                            >  2:621109/1‑147 (MQ=255)
                                                                                                     ttACTGGCTTCTTTCTTCTACGCATGCCCTGGGCAACTTTCCGCGCTTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATTGATAACCTACCATGTAGAAGAACGTTCCTGACCTCTGCAGGCTACTACAGCATAGTTGTCGTTTGa                                           <  2:224855/149‑1 (MQ=255)
                                                                                                      tACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGAc                                          <  1:98469/149‑1 (MQ=255)
                                                                                                       aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACt                                         <  1:523728/149‑1 (MQ=255)
                                                                                                        ttggagttttcagcgctgttaccccgtgggcaatctttagacccTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAg                                                      <  1:116398‑M2/91‑1 (MQ=255)
                                                                                                        ttggagttttcagcgctggtaccccgtgggcaatctttagacccTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAg                                                      >  2:116398‑M2/45‑135 (MQ=255)
                                                                                                           gCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATCCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATc                                     <  1:278061/149‑1 (MQ=255)
                                                                                                           gCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATACCGCCGGTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCAAGCCTACTACAGCATAGTTGCCGTTTGCCTTATc                                     >  2:415180/1‑149 (MQ=255)
                                                                                                              tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTTTCGTTTGACTTATCCGc                                  >  2:56140/1‑149 (MQ=255)
                                                                                                              tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGc                                  <  1:507301/149‑1 (MQ=255)
                                                                                                               cGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCt                                 <  1:473629/149‑1 (MQ=255)
                                                                                                                 ttttCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTgg                               >  1:4671/1‑149 (MQ=255)
                                                                                                                      tttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGGTGTTTGACTTATCAGCTGGggctt                          >  1:570558/1‑148 (MQ=255)
                                                                                                                           cATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGCCTTATCAGCTGGGGCTGGCTGa                     >  1:16813/1‑149 (MQ=255)
                                                                                                                               tccTGGGCACTGTGCCGCGCCTACCCACCAGGCGTTCTTATAAACGCCTCCATTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTGGGGCTGGCTGAACAt                 <  1:612459/148‑1 (MQ=255)
                                                                                                                               gCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTGGGGCTGGCTGAACAt                 <  2:215916/149‑1 (MQ=255)
                                                                                                                                 gtgggcaagctgccgacccTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTGGGGCTGGCTGAACATCa               <  2:624090‑M2/130‑1 (MQ=255)
                                                                                                                                 cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGACGACGCTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTGGGGCTGGCTGACCATCa               >  1:334180/1‑149 (MQ=255)
                                                                                                                                  tGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTGGGGCTGGCTGAACATCAg              <  1:209758/149‑1 (MQ=255)
                                                                                                                                      cAATTTGCCGCGCCTACCCACCAGGCGGTGTGATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTGGGGCTGGCTGAACATCAGGAGc          <  2:237161/149‑1 (MQ=255)
                                                                                                                                          ttGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTGGGGCTGGCTGAACATCAGGAGCCGGt      <  1:425322/149‑1 (MQ=255)
                                                                                                                                             ccGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTGGGGCTGGCTGAACATCAGGAGCCGGTTTa   <  1:289758/149‑1 (MQ=255)
                                                                                                                                              cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTGGGGCTGGCTGAACATCAGGAGCCGGTTTaa  <  1:624653/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
TCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTTCCTGACCTTTCCAGGCTACTACAGCATAGTTGTCGTTTGACTTATCAGCTGGGGCTGGCTGAACATCAGGAGCCGGTTTAA  >  NC_000913_3_hsa_tpiA/3468938‑3469228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.