Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 264,098 G→T 100% L50L (CTC→CTA)  ykfH ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA264,0980GT100.0% 116.9 / ‑6.7 44L50L (CTC→CTA) ykfHuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (25/18);  minor base C (0/1);  total (25/19)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATTT  >  NC_000913_3_hsa_tpiA/263952‑264243
                                                                                                                                                  |                                                                                                                                                 
cGGGCTCCCAGGCGCAGTGTAACCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAg                                                                                                                                                 <  1:551073/149‑1 (MQ=255)
   gCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGAc                                                                                                                                              >  1:804910/1‑149 (MQ=255)
            cgcAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGat                                                                                                                                     >  1:432317/1‑149 (MQ=255)
              cAGTGTAATCTCCCGCTGCAGGCCCCTGGGAGGCATCGTAATTACAGTTCTCCACTATTGTCAGTTCAGAGTGATGCTCATCAGGCACGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGatat                                                                                                                                   >  2:141847/1‑149 (MQ=255)
               aGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATc                                                                                                                                  <  1:208217/149‑1 (MQ=255)
                        tcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTGCACTATTGTCAGTTCGGGGTGATGCTCATCAGGCGGGTATAGGGCCCATTGCGGTCTTAGCGGCGTTCGGCGTATCCCGCTAGGACTCCGGCGATATCCGGAACGTc                                                                                                                         >  1:743272/1‑149 (MQ=255)
                          ccGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTccc                                                                                                                       >  1:598904/1‑149 (MQ=255)
                          ccGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGCTCGGCGTCTACCGCTAGGACTCCGGCGATACCCGGAACGTccc                                                                                                                       >  2:566626/1‑149 (MQ=255)
                                cAGGCCCCTGGTAGGGTTGCTCATTACAGATCTCCGCAATATACAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAGCGTCCCTGACgg                                                                                                                 >  1:199313/1‑149 (MQ=255)
                                  ggCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                                                                                                               <  1:651035/149‑1 (MQ=255)
                                      gcTGGTAGGGGTGGTCAGGACAGTGCTCGACGATGGTCAGTGCAGGGGGAGGCGCATGAGGGGGGTATAGGGCCCAGTGCGGTCCGGGGGGCGGTCGGCGTATACCGCTAGGGGTCGGGCGAGATCCGGAACGTCCCTGCCGGTGTGAt                                                                                                           <  2:373711/148‑1 (MQ=255)
                                        tGGTAGGGTTGCTGATTACAGTTTTCCACTATTGTCGGTTCAGGGGGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATgc                                                                                                         <  2:422978/149‑2 (MQ=255)
                                          gTAGGGTTGCTCATTACAGTTCTCCTCTATTGGCAGTTCAGGGTGAGGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACa                                                                                                       <  1:346902/149‑1 (MQ=255)
                                            aGGGTGGTTCACTAAAGTTCTCCACTAGTGGCAGTTGAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATCGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTTCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGa                                                                                                     <  2:237497/149‑1 (MQ=25)
                                              ggTTGCTAATTACAGTTCACCACAATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATAGCGCTAGGACTCCGGAGATATCCGGAACTTCCCTGCCGGTGTTATGACAGACg                                                                                                   >  1:776919/1‑149 (MQ=25)
                                                          cAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCAc                                                                                       <  2:598904/149‑1 (MQ=255)
                                                            gTTCTCCACTATTGTGAGTTCAGGGTGATGCGCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTg                                                                                     <  2:151749/149‑1 (MQ=255)
                                                             ttCTCTACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCTGGCCACTgt                                                                                    >  1:527648/1‑148 (MQ=255)
                                                             ttCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTgg                                                                                    >  1:375587/1‑149 (MQ=255)
                                                                tcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAt                                                                                 >  1:81759/1‑149 (MQ=255)
                                                                  cACTCTTGTCAGCTCAGCGTGTTGCTCATCAGTCAGTTATAGGGCGCAGTGCGGGCCGGGCGGCGTGCGGCGTAGACCGGTAGGACTCCGGCGATATCCCGAACGTCCCGGCCGGTGTAATGACAGACGCTACGGTGCCACTGGTAttt                                                                               <  2:224269/149‑1 (MQ=18)
                                                                  cACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGGACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAttt                                                                               >  1:389536/1‑149 (MQ=255)
                                                                       ttGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAAGGACAGACGCTCCCGTGCCCCTGGTATTTGCCgg                                                                          >  1:835928/1‑149 (MQ=255)
                                                                          gcAGTGCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGATAGGACTCCGGCGATAGACGGAAGGTCCCTGCCGGTGTAATGACAGCCGCCACCGTGACACTAGTATTTTCCGGTGc                                                                       >  2:61555/2‑149 (MQ=255)
                                                                                        tGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCTGTAGCGAAAGGtt                                                         >  1:132454/1‑149 (MQ=255)
                                                                                             atcaGGCAGGGATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATGCCGCTCGGACGCCGGCGATCTCCGGAACGTCCCTGCCGGGATAATGACAAACGCTACCGTGCCACTGGTTTGTGCCGGTGCAGTGGCGAAAGGTTCgggc                                                    >  2:253662/1‑148 (MQ=255)
                                                                                                aggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACCTCCCTGCCGGTGTAATGACCGACGCCGACTTGCCCCCGGTATTTTCCGGGGCAATACCTAAGAATTCGGGACTc                                                 >  1:397628/1‑149 (MQ=255)
                                                                                                  gcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAg                                               >  1:118663/1‑149 (MQ=255)
                                                                                                     ggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGAt                                            >  1:246738/1‑149 (MQ=255)
                                                                                                                cATTGCGGTCCTGGCGGCGTTCGGCATATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCGGGATGCTGGCGGtat                                 >  1:434675/1‑149 (MQ=255)
                                                                                                                 aTTGCGGTCCTGGCGGCGTTTGGCGTATATCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGTCAGACGCGACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGtata                                <  1:324798/149‑1 (MQ=17)
                                                                                                                  ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTatat                               >  1:605516/1‑149 (MQ=255)
                                                                                                                     cGGTCCTGGAGGCGGTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCGACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGATAAAGGAGGCTGGCGGTATATCGt                            <  2:687735/149‑1 (MQ=17)
                                                                                                                      ggTCCTGGCGGCGTCCGGCGTATACCGCTAGGACTCCGGCAATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCATTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTc                           <  1:196950/149‑1 (MQ=255)
                                                                                                                       gTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCa                          <  1:267106/149‑1 (MQ=255)
                                                                                                                              ggtcttgcGGCGTAGACCGCTAGGACGCCGGCGACATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGGCATTGCCCt                   <  2:158643/142‑1 (MQ=14)
                                                                                                                                  gTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGGATATCGTCATTGCCCTGCGt               >  1:179063/1‑149 (MQ=255)
                                                                                                                                   tccGGCGTATACCGCCAGGACTCCGGCCATAACCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGtt              <  1:294391/147‑1 (MQ=255)
                                                                                                                                     cGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTa            <  2:605516/149‑1 (MQ=255)
                                                                                                                                      ggCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTAc           >  1:696910/1‑149 (MQ=255)
                                                                                                                                       gCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTGTAGCGTCCTTGCCCTGCGTTTACt          >  1:632526/1‑149 (MQ=255)
                                                                                                                                         gTATACCGCTAGGACTCTGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCCAAAGATTCGGGGCCCAGGACGCTGGCGGTATCTCGTCATTGCCCTGCGTTTACTGa        <  2:621177/149‑1 (MQ=17)
                                                                                                                                            tgccGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGCCGCTACCGTGCCACTGGTATTTGACGGTGCAGTAGCGTAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATaa     <  1:808445/147‑1 (MQ=255)
                                                                                                                                            taCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCCGGATGCTGGCGGTATCTTCCCCTTGCCCGCCGTTTACGGATaa     >  2:789523/1‑149 (MQ=14)
                                                                                                                                               cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTGCCGTGCCAGTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAAttt  >  2:255388/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATTT  >  NC_000913_3_hsa_tpiA/263952‑264243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑