Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 3,202,613 G→T 7.3% M241I (ATG→ATT)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA3,202,6130GT 7.3% 167.1 / 4.6 70M241I (ATG→ATT) ccafused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
Reads supporting (aligned to +/- strand):  ref base G (31/32);  new base T (2/3);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

TGTTCTTCCAGGTACTGCGCGATTGCGGCGCACTGCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGT  >  NC_000913_3_hsa_tpiA/3202465‑3202759
                                                                                                                                                     |                                                                                                                                                  
gcttctccCAGGTACTGCGCGATTGCGGCGCACTGCGCGTCTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGCGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATg                                                                                                                                                    <  2:44824/147‑1 (MQ=255)
  ttgttcCAGGTACTGCGCGATTGCGGAGCAATTCGGGTTTTAGGACCGGAAATTGATGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTGATACCTTACTGAGGTTCTCACTGGAGGTGATGcg                                                                                                                                                  >  2:141611/4‑148 (MQ=255)
  ttcttcCAGGTACTGCGCGATTGCGGCGCACTGCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCt                                                                                                                                                  <  2:500301/149‑1 (MQ=255)
  ttcttcCAGGTACTGCGCGATTGCGGCGCACTGCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCCCAATGGCGGCGATGCt                                                                                                                                                  >  2:51753/1‑149 (MQ=255)
    cttcCAGGTGCTGCGCGTGTGCGGCGCACTGCGCGTTTTATGCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGa                                                                                                                                                <  2:67207/149‑1 (MQ=255)
    cttcCAGGTACGGCGCGATTGCGGCGCACTGCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGa                                                                                                                                                <  1:121108/149‑1 (MQ=255)
     ttcCAGGTACTGCGCGATTGCGGCGCACTGCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAg                                                                                                                                               >  1:557529/1‑149 (MQ=255)
     ctcCAGGTACGGGGCGATGGCGGCGCACTGCGCGTTTTATTCCCGGAAATTTACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAg                                                                                                                                               <  2:819969/148‑1 (MQ=255)
       ccAGGTACTGCGCGATTGCGGCGCCCTCCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTc                                                                                                                                             <  2:614338/149‑1 (MQ=255)
         aGGTACTGCGCGATTGCGGCGCACTGCGCGTTTTATTCCCGGAAATTAACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATGCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTGCg                                                                                                                                           >  2:435494/1‑149 (MQ=255)
           ggaCTGAGCGATTGCGGCGCACGGCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAAGTGCGGCGATGCTGAGTCCGCa                                                                                                                                         <  2:182012/147‑1 (MQ=255)
                  gcgATTGCGGCGCACTGCGCGTTTTATTCCCCGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGAt                                                                                                                                  <  2:323278/149‑1 (MQ=255)
                         cggcgCACTGCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGtt                                                                                                                           >  1:494879/1‑149 (MQ=255)
                          ggagcAATGCGCGTTTTATTCCCGGAAATTGAAGCACTGATGGGAGTTCCGACCCCTGCCCAGTGGCATCCGGAAAT‑CGATACGGGCAGTAATACCTTGATGAGGGTATCAATGGCGTCGATACTGTGTCCGCCGGTCGAGGTCGGttt                                                                                                                          >  2:698470/4‑149 (MQ=255)
                           gcgcACTGCGCGTTTTATTCCCGGAAATTTACGCACTTTCTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTc                                                                                                                         <  2:401126/149‑1 (MQ=255)
                           gcgcACTGCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGAATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTc                                                                                                                         <  1:444103/149‑1 (MQ=255)
                             gcACTGCGCGTTTTATTCGCGGAAATTGACGCACTGGTTGGCGTTCCGGCCCCTGCCAAGTGACATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGc                                                                                                                       <  2:747509/149‑1 (MQ=255)
                               aCTGCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATTCGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCaa                                                                                                                     <  1:534360/149‑1 (MQ=255)
                                 tGCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGAGGCTGAGTCCGCAGGTCGATGTCCGTTTTGCAACt                                                                                                                   >  1:764765/1‑149 (MQ=255)
                                 tGCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCCCCGCACGCCCAGGGCCATCCGGAAAT‑CGATACCGGTATTCATACCTTAATGTCGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGCCCGTTTCGCAACt                                                                                                                   <  1:24055/149‑1 (MQ=255)
                                  gcgcGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACtt                                                                                                                  >  2:643934/1‑149 (MQ=255)
                                      gTTTTATTCCCGGAAATTGACGCACTGTTTGGTGTTCCGGCCCCTGCCAACAGGTTGTCGTGAGT‑CGATACGGGTATTCAGACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATg                                                                                                              <  1:400635/149‑1 (MQ=255)
                                      gTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATg                                                                                                              <  1:11323/149‑1 (MQ=255)
                                            ttCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGa                                                                                                        >  1:264946/1‑149 (MQ=255)
                                             tCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATAACTTAATGACCCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGag                                                                                                       >  1:747211/1‑148 (MQ=255)
                                                cGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGAtctc                                                                                                    <  1:576865/149‑1 (MQ=255)
                                                cGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGAtctc                                                                                                    >  2:514366/1‑149 (MQ=255)
                                                        gaagccttGTTTGGCGTCCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCACTCAATGGCGGCGATGCCGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAgg                                                                                            <  1:567655/142‑1 (MQ=255)
                                                        gACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAgg                                                                                            <  1:567637/149‑1 (MQ=255)
                                                             aCTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGTTATTCATACCTGAATGACGCTCTCAATGGCCGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACAATCCCGGCGAAGGGCTGa                                                                                       >  2:137767/1‑149 (MQ=255)
                                                             aCTGTTTGGCGTTCCGGCCCCTCCCACGTGGCTTCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCCCTCAATGGCGGCGATCCTGAGTCCGCAGGTCGATGTCCGTTTCGCGCCTTTATGCCACGCTCCCGGTAATGGGcctg                                                                                       >  2:514355/1‑146 (MQ=255)
                                                             aCTGTTTGGCGTTCCGGCCCCGGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGa                                                                                       <  1:682081/149‑1 (MQ=255)
                                                                 tttGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCACTGGCGGCGTTGCTTAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCTGTCTCTCGGTAAAGGGCTgcggcc                                                                                   >  2:209927/1‑144 (MQ=255)
                                                                   gggCGTTCCGGCCGTTGACAAGGGGCAT‑CGGATATGTGATCCGGTTATGTATACTGTAATGACGCTCTCAATGCCGGCGATTCTTACTCCGCTGGTCGATGTCCGTCTCGCAACTTTATTCCACGATCTCGGTAAAGGGCTGAcgccgc                                                                                 <  2:274173/148‑1 (MQ=255)
                                                                        ttCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGaa                                                                            <  1:148889/149‑1 (MQ=255)
                                                                           cGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATAGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAActc                                                                         >  1:731790/1‑149 (MQ=255)
                                                                              cgccTGCCGAGTGGAATACGGAAAG‑CGATAGGGCTCGTCATACCTCAAGCGTGCTCTCAAGTGCAGCGATGCCGAGGCCTCAGATCGTTGTCCGCTTCCCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTgg                                                                      <  2:800140/147‑1 (MQ=255)
                                                                              ccccTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAAGGGCGGCGATGATGAGTCAGCAGGTCGATGTCCGTTTCGCAACTTTATGACAGGATATCGGTCAGGTACTGTCGCCCCGAGAACTCggg                                                                      >  1:187569/1‑149 (MQ=255)
                                                                               cccTGCCAAGTGGCATCCGGAAAT‑CGATCCGGGTATTCATACCTTCATGTCGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCCACTTTATGCCACGAACTCGGTAAACGCCTGACGACCCCAGAACCCTGGc                                                                     >  2:338934/1‑149 (MQ=255)
                                                                                  tGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCGTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCCGGTCGATGTCCGTTTCGCAACTTTATGCCACGATGTCGGTAAAGGGCTGCCGCCGCCCGAActccgccccc                                                                  >  2:241391/1‑142 (MQ=255)
                                                                                        gTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGCCGCCGCCAGCACTCTGGCCGCGtcatc                                                            >  2:409979/1‑149 (MQ=255)
                                                                                             acacGGAAAT‑CGACACGGGTATTCGTACCTTAATCACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggt                                                       <  1:345003/146‑1 (MQ=255)
                                                                                             aTCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATTCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCa                                                                               >  2:280641/1‑125 (MQ=255)
                                                                                             aTCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATTCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCa                                                                               >  2:279876/1‑125 (MQ=255)
                                                                                             aTCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATTCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCa                                                                               <  1:279876/125‑1 (MQ=255)
                                                                                             aTCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATTCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCa                                                                               <  1:280641/125‑1 (MQ=255)
                                                                                             aTCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggt                                                       <  1:459124/149‑1 (MQ=255)
                                                                                              tCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGCTCGCGGTAAAGAGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcgtggtc                                                      >  2:447037/1‑149 (MQ=255)
                                                                                              tCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCAGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtc                                                      >  2:708543/1‑149 (MQ=255)
                                                                                                  gAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtcatgg                                                  <  1:419895/149‑1 (MQ=255)
                                                                                                     aT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGccc                                               >  2:564435/1‑149 (MQ=255)
                                                                                                         gATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCCTGGCCcggc                                            >  1:311890/1‑149 (MQ=255)
                                                                                                           tACGGTTATTCCTACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCGTCATGGTCATGGCCcggcgg                                          <  2:473451/149‑1 (MQ=255)
                                                                                                           tACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGATCGATGGCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCAGGGTCATGGCCcggcgg                                          >  1:161651/1‑149 (MQ=255)
                                                                                                            aCGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCggg                                         <  1:372740/149‑1 (MQ=255)
                                                                                                              gggTATTCATACCTTAATGACGCTCTAAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGACCCAAGAACTCTGGCCGCGTCCTCATGGTCATGGCACGGCgggca                                       >  2:460524/1‑147 (MQ=255)
                                                                                                                gTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGtt                                     >  1:425505/1‑149 (MQ=255)
                                                                                                                        aCCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAACGGACTGACGCCGCCCGACCTCTGGCCCCGTCATCAGAGTCATGGCCCTGCGGGGGTTAAGTTAGt                             >  2:229292/1‑149 (MQ=255)
                                                                                                                        aCCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGt                             >  1:350507/1‑149 (MQ=255)
                                                                                                                        aCCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCACGTCGATGTCCGTTTCGCAACTTTATGCCCCCATCCCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCCGCGGCTGTTAACTTAGt                             >  2:177260/1‑149 (MQ=255)
                                                                                                                          cTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGCTGTCCGTTCCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTgg                           <  1:784499/149‑1 (MQ=255)
                                                                                                                          cTTAATGACGCTCTCAATGGCGGCGAAGCTGAGCTGTTTATTTTATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGGTAGTgg                           >  1:199397/1‑149 (MQ=255)
                                                                                                                                     tctcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCCCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGc                >  1:167539/1‑149 (MQ=255)
                                                                                                                                       tcAATGGTGGCGATGCCGAGGCCGAAGGGGGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCa              <  1:95168/149‑1 (MQ=255)
                                                                                                                                       tcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGCGTTCAGTTAGTGGAACAACTATGccc              >  1:56566/1‑148 (MQ=255)
                                                                                                                                        cAATGGCGGCGATGCTGAGTCCGCAGGTCGATTTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAg             <  2:713000/149‑1 (MQ=255)
                                                                                                                                           tGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGt          >  1:590264/1‑149 (MQ=255)
                                                                                                                                            ggcggcGATGCTGAGTCCGCAGGTCTATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGATCTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTc         <  1:759388/149‑1 (MQ=255)
                                                                                                                                              gggcgATGCGGAGTCCGCAGGTCGATGTCCTTTTCGCAACTGTACGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGATCTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTg       <  2:26881/148‑1 (MQ=255)
                                                                                                                                                 cgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGGAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTCGAACAACTATGCCAGCGTCTgcgc    <  1:725831/149‑1 (MQ=255)
                                                                                                                                                   aTGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGt  <  1:713489/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TGTTCTTCCAGGTACTGCGCGATTGCGGCGCACTGCGCGTTTTATTCCCGGAAATTGACGCACTGTTTGGCGTTCCGGCCCCTGCCAAGTGGCATCCGGAAAT‑CGATACGGGTATTCATACCTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGT  >  NC_000913_3_hsa_tpiA/3202465‑3202759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑