New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	4465486 =	28 (0.970)	5 (0.180)	4/264	3.4	16.8%	coding (677/1422 nt)	treB	trehalose‑specific PTS enzyme: IIB and IIC component
	?	NC_000913_3_hsa_tpiA	4465514 =	23 (0.850)					coding (649/1422 nt)	treB	trehalose‑specific PTS enzyme: IIB and IIC component
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCGAGGAACACCGCGAGGATCAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGA  >  NC_000913_3_hsa_tpiA/4465342‑4465631                                                                                                                                                 |                                                                                                                                                  gCGAGGAACACCGCGAGGATCAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGTGGTCACCTGCGCCTGATAGCCTACttt                                                                                                                                               >  2:135151/1‑149 (MQ=255)    aGGAATACCGCGATGATTAGCGGACTGACGGGTTCCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGTGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGc                                                                                                                                            <  1:155020/149‑1 (MQ=255)     ggAACACCGCGAGGATCAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCCGCGCCAGTCCGGCTAACAGTGCCGGGATAACCTGAGCCTGGTAGCCTACTTTGGCg                                                                                                                                           >  1:393257/1‑149 (MQ=255)         cacCGCGAAGATCAGCGAACAGACGGATACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGc                                                                                                                                       <  1:82983/149‑1 (MQ=255)                  gATCAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGc                                                                                                                              <  1:299274/149‑1 (MQ=255)                        cGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGt                                                                                                                        <  1:125901/149‑1 (MQ=255)                            cAGACGGCTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCca                                                                                                                    <  2:478151/149‑1 (MQ=255)                              gACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCcaca                                                                                                                  <  1:400717/149‑1 (MQ=255)                                                    agGTAATCCGGGACGATGGGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAg                                                                                            <  2:488767/149‑1 (MQ=255)                                                          tCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGGTa                                                                                      >  2:138780/1‑149 (MQ=255)                                                             ggCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGAGAGCCTACTTGGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGc                                                                                   <  1:227349/149‑1 (MQ=255)                                                                        tttagtgCGGGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCt                                                                        <  2:206879/143‑1 (MQ=255)                                                                        tttAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGAGAATGGTAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCt                                                                        >  1:498873/1‑149 (MQ=255)                                                                             ggCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGTCCGAGCAGATAAGCGTTCATCAGCTGTTga                                                                   >  2:109391/1‑149 (MQ=255)                                                                             ggCGAGTTTAAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGga                                                                   <  2:48859/149‑1 (MQ=255)                                                                              gCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTACCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACAATTCCGGCAGCTGCTGCCCGAGCAGATAACCGTTCATCAGCTGTGgag                                                                  >  1:100497/1‑149 (MQ=255)                                                                                      cAATAACGCCCAGCGCCCGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAgt                                                          <  1:423131/149‑1 (MQ=255)                                                                                        aTAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGTAGATAAGCGTTCATCAGCTGTGGAGAAACCAgtgt                                                        <  1:273009/149‑1 (MQ=255)                                                                                                       gttCCGGCTAACAGTGCCGGGATCACCCGCGAAAAAACGTCAACTTTTTCTATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGTAGATAAGCGTTCATCTGCTGTGGAGAAACCAGTGTCACGCCAAGCACGAt                                         <  2:236104/147‑1 (MQ=255)                                                                                                       aGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCTGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGAt                                         <  1:441164/149‑1 (MQ=255)                                                                                                            ggCGAACAGTGCCGGGATCACCTGCGCCTGATAGGCTACGTTGGGGATGCTGAACATGCCAAAGGCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCGGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCaa                                    <  2:245389/149‑1 (MQ=255)                                                                                                                    gtccctggATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGc                            <  1:173293/143‑1 (MQ=255)                                                                                                                                     gcCTGATAGTCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCAttttttt           <  1:254851/149‑1 (MQ=255)                                                                                                                                         gATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCAcc       >  1:59208/1‑149 (MQ=255)                                                                                                                                          aTAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCg      <  2:27543/149‑1 (MQ=255)                                                                                                                                            aGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCt    <  1:135151/149‑1 (MQ=255)                                                                                                                                              ccTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCtaa  >  2:19029/1‑147 (MQ=255)                                                                                                                                              ccTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGa  >  2:474787/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GCGAGGAACACCGCGAGGATCAGCGAACAGACGGGTACCACCACCAGATAGAGGTAATCCGGCACGATGCGTTTAAGGCGAGTTTCAATAACGCCCAGCGCCAGTCCGGCTAACAGTGCCGGGATCACCTGCGCCTGATAGCCTACTTTGGCGATGCTGAACATGCCAAAGTCCCACACTTCCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGA  >  NC_000913_3_hsa_tpiA/4465342‑4465631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.