Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 2,088,156 T→C 11.2% S35G (AGT→GGT)  yeeY ← LysR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA2,088,1560TC11.2% 91.5 / 2.4 46S35G (AGT→GGT) yeeYLysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (17/19);  new base C (2/3);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

ACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAGCGCGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTT  >  NC_000913_3_hsa_tpiA/2088029‑2088304
                                                                                                                               |                                                                                                                                                    
aCGGTATGCAGAACTTCCCGCCGTTTCTCGAATAACATTTTTCCGGTGCTGGTGAATTTAGCGCCGTGTCCGATACGCTCAAGCCATTGAATATTACGGCCGCTTTCCCGCTTGTGATCGGTTTATCCCTGAGCGGACGGCGTCTTGTa                                                                                                                                 <  2:162009/149‑1 (MQ=255)
aCGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAGCGCGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTa                                                                                                                                 <  1:525725/149‑1 (MQ=255)
       gCAGAACTTCCCGCCCTTTCGCTAATAACATTTTTCCGGTGCGGGTGAATTTAGCGCGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTg                                                                                                                          <  1:180659/149‑1 (MQ=255)
       gCAGAACTTCCCGCCCGTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAGCGCGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTATAGAGTTTg                                                                                                                          >  2:509360/1‑149 (MQ=255)
              ttCCCGCCCTTGCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAGCGCGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACGCAGAGCGGGCGGCGGCTTGTAGAGTCTGGCCGGcg                                                                                                                   >  1:419720/1‑149 (MQ=255)
                   gCCCTTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAGCGCGGTGTCCGCTACGATGAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTGACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGAcgccgcc                                                                                                              >  1:170946/1‑149 (MQ=255)
                          ctctAATAACATTTTTCCGTTTCTGGTGAATTTAGCGCGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACCCAGAGCTGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAAc                                                                                                       >  2:91102/1‑149 (MQ=255)
                          cgctAATAACATTTTTCCGGTGCGGGTGAATTTAGCGCGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACCCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAAc                                                                                                       >  2:91448/3‑149 (MQ=255)
                                    aTTTTTGCGGGGCGGGTGAATTTAGCGCGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCttt                                                                                             <  2:672878/149‑1 (MQ=255)
                                      ttttCCGGTGCGGGTGAATTTAGCGCGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTACCTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCttttt                                                                                           <  1:481019/149‑1 (MQ=255)
                                        ttCCGGTGCGGGTGAATTTAGCGGGGTGTCTGCTAGGATCAAGCAATTGGTTGTTAGGGTGGCTTTTGTGGTTGTGAGTGGTGTAGCTCAGAGTGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCCAAATTGACTTCTTTTTCt                                                                                         >  2:15668/1‑149 (MQ=255)
                                          ccGGGGCGGGCGAATTTACCGCGGTGCCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTaa                                                                                       <  1:416322/149‑1 (MQ=255)
                                           cGGGGCGGGTGAATTTAGCGCGGTCGCCGCTACGAGCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGCCTTGTATAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAg                                                                                      <  2:237303/149‑1 (MQ=255)
                                                   gTGAATTTATCGCGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAg                                                                              <  2:206335/149‑1 (MQ=255)
                                                   gTGAATTTAGCGCGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAg                                                                              >  1:204428/1‑149 (MQ=255)
                                                    tgattctAGCGCGGTGTTCGCTATGATCAAGCAATTGAATATTAAGGTCGCTTTCCTGTTTGTGAACGGTGTAACGCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTTTTTTTCTAAGGCATCGAgg                                                                             <  2:332313/143‑1 (MQ=255)
                                                       aTTTAGGGCGGTGTTCGCAACGATCAAGCAATTGAATATTAGGGTCGCTTTCCAGTGGGTGGAAGGTGTGACGCAGAGCGGGCGGCGTCGTGTAGGGTTTGGCCGAGGCCGCCGCAAAGCTTCCTTCTTTTTCTAAGGCATCGAGGatc                                                                          <  2:48262/149‑1 (MQ=255)
                                                            gcgcGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCaa                                                                     <  1:402789/149‑1 (MQ=255)
                                                             cgcgATGTCCGCTACGATCAAGCTATTGAATATTAAGGTCGCTTTACAGCTTGTGAACGGTGTAACTCAGAGCGGACGTCGTCTTGTAGAGCTTGGCCCACGCCCCCGCAGAACTGCCTGCTTTTTCTAAGGCATCGAGGATCATCAac                                                                    <  1:581022/149‑1 (MQ=255)
                                                                     ccGCTACGATCAAGCAATTGAATATTAAGGTCGCGTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCCTGTAGAGTTGGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCcag                                                            <  2:380284/149‑1 (MQ=255)
                                                                         tACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGt                                                        >  2:617490/1‑149 (MQ=255)
                                                                                 aGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCAt                                                >  1:216939/1‑149 (MQ=255)
                                                                                  gCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATa                                               >  2:153758/1‑149 (MQ=255)
                                                                                  gCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGCAGAGTTTGGCCGACGCCGCCGCAAAACTTCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATa                                               <  1:628286/149‑1 (MQ=255)
                                                                                     aTTGCATATTAAGGTCGCTTTCTAGCGTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATacac                                            <  1:498630/149‑1 (MQ=255)
                                                                                     aTTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATacac                                            >  1:402735/1‑149 (MQ=255)
                                                                                      ttGAATGTAAAGGTCGCTTCCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGGTGGGCGGACGCCGCCGCAAAAATGCCTCCTGTTTCTACGGCATCGAGCGTCATCAACACATCCCACGGAGGTTCCATaccag                                           >  2:447147/1‑146 (MQ=255)
                                                                                      ttGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAGGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATAcgcg                                           >  1:221109/1‑149 (MQ=255)
                                                                                           tatTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACGCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCCTCGAGGATCATCAACACATCCAGCCGTGGTTTCATACAAGccccc                                      >  2:384041/1‑149 (MQ=255)
                                                                                           tatTAAGCTCCGTTTCCAGCGTGTGAACGGTGTAACTCCGAGCTGACGGCGTCTTGTAGTGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCAGCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGccccc                                      <  2:222514/149‑1 (MQ=255)
                                                                                           tatTAACGTCGCTTTCCAGCTTCTGAACGGCGTAACTAAGCGAGGCCGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTTCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGccccc                                      <  1:229617/149‑1 (MQ=255)
                                                                                                aGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCGGAGCGGACGTCTTTTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCa                                 <  1:125853/149‑1 (MQ=255)
                                                                                                  gTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGt                               <  2:512211/149‑1 (MQ=255)
                                                                                                      cTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACGGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCAt                           >  2:480201/1‑149 (MQ=255)
                                                                                                          ccATCTTTTGAAGGGGGGATCTCAGGGCGGTCGGCGTCTTGGAGAGGTTGGCCTACGCCGCCGCAAAACTGCCTTCTTTTGCGAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATgcgg                       <  1:657496/149‑1 (MQ=255)
                                                                                                           cAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGACTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACAACCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATgcggc                      >  2:480372/1‑149 (MQ=255)
                                                                                                            aGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATgcggcg                     >  2:207429/1‑149 (MQ=255)
                                                                                                            aGCTTGTGAACGGTGTAACTCAGAGCGGACGACGTCTTGGACAGTTGGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTAGCGGTGCATgcggcg                     >  2:233305/1‑149 (MQ=255)
                                                                                                            aGCGTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGCTTCATACACCCCCCCTTGCAGTGCATgcggcg                     >  2:440741/1‑149 (MQ=255)
                                                                                                                 gtgAACGGTGTAACCCAGAGCGGACGGCTTGTTGTAGAGGTTGGCAGCCGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCctg                <  1:91102/149‑1 (MQ=255)
                                                                                                                 gtgAACGGTGTAACCCAGAGCGGACGGCGTCTTGCAGAGTTTCGCCGACACCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCctg                <  1:91448/149‑1 (MQ=255)
                                                                                                                  tgAACGGTGTAACTCAGAGCGGACTGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCctgc               <  1:88664/149‑1 (MQ=255)
                                                                                                                  tgAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGGTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACTTCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCctgc               <  1:87080/149‑1 (MQ=255)
                                                                                                                          gtAACGCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGa       <  1:282455/149‑1 (MQ=255)
                                                                                                                             aCTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGCGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCg    >  1:244855/1‑149 (MQ=255)
                                                                                                                             aCTCAGAGCGGACCGCGACTTGTAGAGTTTCGCCGACCCCGCCGCAAAACTGCCTTCTTTTTCTCAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATCCACCCCCCCTCGCAGTGCATGCGCCGTCCTGCTGGCAGGATCg    >  2:392030/1‑149 (MQ=255)
                                                                                                                               tCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCCTTCGGCGTCCTGCTGGCGTGATCGtt  >  1:364885/1‑149 (MQ=255)
                                                                                                                               tCAGAGCGGACCGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGCTCATCAACACATCCAGCAGTGGCTGCATACACGCCCCCTTGCAGTGCATGCGGCATCCTGCTGGCGTGACCGtt  >  2:136426/1‑149 (MQ=255)
                                                                                                                               |                                                                                                                                                    
ACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCGGGTGAATTTAGCGCGGTGTCCGCTACGATCAAGCAATTGAATATTAAGGTCGCTTTCCAGCTTGTGAACGGTGTAACTCAGAGCGGACGGCGTCTTGTAGAGTTTGGCCGACGCCGCCGCAAAACTGCCTTCTTTTTCTAAGGCATCGAGGATCATCAACACATCCAGCAGTGGTTTCATACACGCCCCCTTGCAGTGCATGCGGCGTCCTGCTGGCGTGATCGTT  >  NC_000913_3_hsa_tpiA/2088029‑2088304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑