Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_hsa_tpiA 4,296,042 C→T 33.4% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,296,0420CT33.4% 65.3 / 46.9 66intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (23/21);  new base T (12/10);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

GGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATC  >  NC_000913_3_hsa_tpiA/4295897‑4296184
                                                                                                                                                 |                                                                                                                                              
ggATAAGGCTTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc                                                                                                                                             <  1:341341/149‑1 (MQ=17)
   tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGCATAAGGCGTTTACGCCCCTTCCGACATCACTGCCTGa                                                                                                                                          >  2:153116/1‑149 (MQ=11)
   tAAGGCATTTACGCCGCAGCCGGCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa                                                                                                                                          <  2:388661/149‑1 (MQ=11)
       gCGTTTATGCCGCATCCGACATGAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTATCGTGATTTTGTAGGTCGGATAGGGCGGTTACGCCGCATCCGACATCAATGACTGATTCg                                                                                                                                      >  2:435020/1‑149 (MQ=11)
       gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGCTGCg                                                                                                                                      >  1:315094/1‑149 (MQ=17)
         gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTGGGATAAGGCGTTGACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                                                                    <  1:544328/149‑1 (MQ=12)
          tttATGCCGCATCCGACATCAACGCCTGCTGCGCCGCTTAACGCGTCTTATCCGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGCTTTTGTAGGTCGGATAAGGCGATTACGCCGCCTCCGACATCACTGCCTGATGCGACg                                                                                                                                   >  1:398859/1‑149 (MQ=11)
             acgCCGCACTCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTTCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt                                                                                                                                <  2:259610/147‑1 (MQ=11)
                 cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGGTTTAGCGTGATTTTGTAGGTCGGATAATGCGTTTACGCCGCATCCGACATAGATGCCTGATCCGACGCCTGCcg                                                                                                                            >  2:541119/1‑149 (MQ=11)
                 cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCACCCGACACCAACGCCTGATGCGACGCTTGCcg                                                                                                                            >  1:346773/1‑149 (MQ=25)
                  gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgc                                                                                                                           <  1:148503/149‑1 (MQ=37)
                    aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATCAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                         >  2:652598/1‑149 (MQ=38)
                     tCCGGCATCAACGCCTGATGCGGCGATTAACGTGTCTGATGAGGCCTATGGCAGTCAGCGCAATAGCCTGTTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                                                                        <  2:269905/149‑1 (MQ=11)
                        gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                     >  1:73322/1‑149 (MQ=25)
                        gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGCCGGATCAGGCGTTTCCGCCGCATCCGACATCAACGCCTGACGCACCGCTTGCCGCGTCTTa                                                                                                                     >  2:512239/1‑149 (MQ=11)
                         aCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCCATAGCCTGATTTAGAGTGATTTTGTAGATCGGATAAGACGTTTACGCCGCATCCGACATCAATACCTCATGCGACGCTTACCTCGTCTTAt                                                                                                                    >  1:149805/1‑149 (MQ=11)
                           aTCAACGCTTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACATCGCAATAGCCTGATTTAGCGTGATTGTGTATGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                  <  1:610166/149‑1 (MQ=11)
                            tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGGCGCTTGCCGCGTCTTATcgg                                                                                                                 >  1:348980/1‑147 (MQ=255)
                            ccAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAGTTAGCGTGATGTTGGAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGCTGCGACGCTTGCCGCGTCTTATCAg                                                                                                                 <  1:658398/148‑1 (MQ=11)
                               aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                              >  2:466084/1‑149 (MQ=17)
                               aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGGCGCTTGCCGCGTCTTATccagcc                                                                                                              >  1:244798/1‑144 (MQ=255)
                                    tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCAGACATCAATGACTGATTCGACGCTTGCCGCTTCTTATCCGGCCTatct                                                                                                         >  2:332251/1‑146 (MQ=11)
                                     gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCCGCCCTACGcc                                                                                                        >  1:263162/1‑149 (MQ=255)
                                     gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                                                                        >  1:263784/1‑149 (MQ=38)
                                        gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGa                                                                                                     <  1:4721/149‑1 (MQ=255)
                                              cTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCCACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGTCAgcgg                                                                                               >  1:554086/1‑148 (MQ=255)
                                              cTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCGgcgc                                                                                               >  2:265423/1‑149 (MQ=255)
                                                 aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACCCTCGACAGCGCAAt                                                                                            >  2:162835/1‑149 (MQ=255)
                                                    tggTCTTATCAGGCCTACGCCAGACCGGGCAAGAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                         <  1:67894/147‑1 (MQ=255)
                                                    gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGAGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                         >  2:519068/1‑149 (MQ=38)
                                                      gTCTTATCAGGTCTACGCCAGACAGCGCAATAGCCTGATTTAGGGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCt                                                                                       <  2:645836/149‑1 (MQ=255)
                                                       tCTTATCAGGCCTACGCCAGACAGCACAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                                                                      <  2:568004/149‑1 (MQ=37)
                                                           aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                   <  1:218917/116‑1 (MQ=25)
                                                           aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                   >  2:218917/1‑116 (MQ=39)
                                                           aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGAAGGACAGCGCAATAGCCTGAttg                                                                                  >  1:183559/1‑148 (MQ=255)
                                                                gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa                                                                                                                                          <  1:41982/88‑1 (MQ=25)
                                                                gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa                                                                                                                                          >  2:41982/1‑88 (MQ=18)
                                                                       caaGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTGGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTg                                                                      <  1:21313/147‑1 (MQ=255)
                                                                             aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                   <  1:29312/98‑1 (MQ=255)
                                                                             aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                   >  2:29312/1‑98 (MQ=255)
                                                                              gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGACAGCGCAATATCCTGCTTTAGCTTGATTTTGTATGTCg                                                               >  2:186054/1‑149 (MQ=11)
                                                                              gcgaAATAGCATGATATACCATACTTTTGCAGGACGCATAACGCGTCTCCGCCGCATCCGTCGTGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg                                                               <  2:674650/149‑1 (MQ=11)
                                                                                gcAATGGCATGATTTAGCTTTATTGTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGa                                                             <  2:227332/149‑1 (MQ=11)
                                                                                   atatCCTTACTTAGCTCGATTTCGTACGTCGTTTAACGCGTTTCCGCCGCGTACGACATCAACGCCTCATGCGACGCTTGCCGCGTCTTATCTGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATaa                                                          <  2:471029/149‑1 (MQ=11)
                                                                                    tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg                                                         <  1:104873/149‑1 (MQ=38)
                                                                                      gCCTGATTTAGCGTGATTTTGTAGGTCGCATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTTGCCCACACACCCCACTAGCCGGATTTCACGTCTTTTTGTGTGCCGGATAATGc                                                       >  2:150159/1‑149 (MQ=11)
                                                                                          gATTTGGCGTGATTTTGTAGGTCGGAAAAGGCTTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGttt                                                   <  1:187836/149‑1 (MQ=255)
                                                                                              tAGCGTGATTTTGTAGTGCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGc                                               <  1:282198/149‑1 (MQ=255)
                                                                                              tAGCGTGATTTTGTAGGTCTGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGc                                               <  1:116862/149‑1 (MQ=38)
                                                                                              gcgggTGATTTTGTAGGGCGGATAAGGCGTTTACGCCGCATCCGACATCACCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGc                                               <  1:226380/145‑1 (MQ=255)
                                                                                               aggttGATTTTGTGGGGCGGAGTAGGCGTGTCCGCCCGATCCGACATCAACGCCTGATGCGAGTCTTGCCGCGTCTTATCAGGCCTACGCTAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTTTGTCGGATAAGGCGTTTATGcc                                              <  2:386635/145‑1 (MQ=11)
                                                                                                gCGTGATTTTGTGGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg                                             <  1:455986/149‑1 (MQ=38)
                                                                                                   tGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt                                                                                                                                         <  1:203865/54‑1 (MQ=255)
                                                                                                      ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGTCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTTTAGGTCGGATAATCCGTTTATGCCGCATCCg                                       >  2:628388/1‑149 (MQ=255)
                                                                                                      ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTATGCCGCGTCCg                                       >  2:628391/1‑149 (MQ=255)
                                                                                                              gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTCTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCaag                               >  1:592080/1‑148 (MQ=38)
                                                                                                               tCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                                           <  1:136940/88‑1 (MQ=255)
                                                                                                               tCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                                           >  2:136940/1‑88 (MQ=255)
                                                                                                                  gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGACTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGAGTTTATGCCGCATCCGACATCAACGcca                           >  1:539412/1‑148 (MQ=255)
                                                                                                                    cctggCATTTACGCCGCATCCTACATCAACTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTTCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGa                         <  1:590749/146‑1 (MQ=11)
                                                                                                                       ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGATTGTCTACGCATCATTTTGTAGGTCGGATAATGCGTTTATTCCGCATCCGACATCAACGCCtactgc                      >  1:157519/1‑144 (MQ=11)
                                                                                                                           tttgccccgcAACCGACATCAACGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGGGTTTTTGTAGTGCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACg                  <  1:65790/143‑1 (MQ=11)
                                                                                                                            ttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCCTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGACAAGGCGTTTACGCCGCATCCGACATCCACACCTGATGCGACGc                 >  2:255322/1‑149 (MQ=11)
                                                                                                                                  cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGGTTTAGCGTGATTTTGTCGGTCGGATAACGCGTTTCGGCCGCATCCCACATGCACGCCTGATGCGAGACTTACcg           >  2:139806/1‑149 (MQ=11)
                                                                                                                                        cGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCtt     >  1:355320/1‑149 (MQ=35)
                                                                                                                                           cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATc  <  1:56240/149‑1 (MQ=35)
                                                                                                                                                 |                                                                                                                                              
GGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATC  >  NC_000913_3_hsa_tpiA/4295897‑4296184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: