Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	4,604,698	C→A	100%	A186E (GCA→GAA)	bglJ →	bgl operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	4,604,698	0	C	A	100.0%	89.8 / NA	33	A186E (GCA→GAA)	bglJ	bgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/18);  total (15/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAC  >  NC_000913_3_hsa_tpiA/4604563‑4604840                                                                                                                                        |                                                                                                                                               tGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTCGCTACGCAAGTAGACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAt                                                                                                                                   <  1:647210/149‑1 (MQ=255)   aaCAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAAAAAAAATTTAAtgt                                                                                                                                 >  2:111411/1‑149 (MQ=255)   aaCAATCAGGTGTCCATTAACCAAATCCGGACGTTAAGCACGACGGAGAGAGAAATATTGCGCTTTACGCCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAACCGATCCGTGAACATAAATTTAAtgt                                                                                                                                 <  2:550551/149‑1 (MQ=255)                aCATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGATTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGATTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTg                                                                                                                    <  2:305868/149‑1 (MQ=255)                  aTTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACCCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAGCGATCCGGGAACATAACTTTAATGTGATGTCGAAACTggg                                                                                                                  >  2:281145/1‑149 (MQ=255)                     atccAAAGCGGGACAGTAAGCCCGACGGAGAGAGAAATATTGCGCGTTATGTCGCGTGGCTACTCAATGATACAAATTGCCGAGCAGCTTAAACGTAATATCAAAACGGTCCGGGAACATAAATTTAATGTGATGTCGAAACTGGGCGt                                                                                                               <  2:129755/147‑1 (MQ=255)                        cAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAg                                                                                                            >  2:458477/1‑149 (MQ=255)                           aGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTc                                                                                                         <  1:233432/149‑1 (MQ=255)                            gCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGGTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCt                                                                                                        <  1:335933/149‑1 (MQ=255)                                    ttAAGCGCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAgg                                                                                                <  2:680173/149‑1 (MQ=255)                                     tAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg                                                                                               >  1:628636/1‑149 (MQ=255)                                          ccGACGGAGAGAGAAATATTGCGCTTTATGTCGTGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttgtt                                                                                          <  2:351436/149‑1 (MQ=255)                                                       aaaTATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCGGCTTAAACGCAATATCCAAAAGATCCGTGAACATAAATTTAATGTGTTGACGAAACTGGGCATCATTTCTGACCCAGGGTTGTTGTATGCCGCGGaa                                                                             >  2:247006/1‑148 (MQ=255)                                                       aaaTATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGCAGTCGAAACTGAGCGTCAGTTCTGACGCAGGGTGGTGGGAGGCCGCAGat                                                                             >  2:603129/1‑149 (MQ=255)                                                                gcTTTATGTCGCGGGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACACAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCACGGTTGTTGGAGGCCGCAGATATTCTGTta                                                                    <  2:624460/149‑1 (MQ=255)                                                                                gtACTTAATGACACAAATTGCCGCTGAGCTTATACGGAATATCAAAAAGATCCGTGAACATAGATTTAATGTGATGTCGAAACTGCGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCg                                                    <  2:535094/148‑1 (MQ=255)                                                                                      aaTGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAGTGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTGGGCGGCCGCCGATATTCTAGATGGTATTGGGCATTGCGCAAcca                                              >  2:471486/1‑147 (MQ=255)                                                                                         gACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTa                                           >  1:205584/1‑149 (MQ=255)                                                                                                   gCCGAGCAGCTTAAACGAAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCa                                 <  1:256800/149‑1 (MQ=255)                                                                                                        ccagcTTAAACGCAAAATGAAGACGAGCCGTGAAAATAAATTTGATGTGATGTCGAAACTGGGCGTCAGTTCTGACGGAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCt                            <  2:136318/148‑1 (MQ=255)                                                                                                            cTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATCGAGTGTCGAAACTGCCCTTTAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGGTATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAGGTGTTGCATCCCTCTTa                        >  1:268485/1‑149 (MQ=255)                                                                                                            cTTAAACGCAATATCAAAACGATCAGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCGTATTa                        >  1:268806/1‑149 (MQ=255)                                                                                                             ttAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAAGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTaa                       >  1:413253/1‑149 (MQ=255)                                                                                                                  cGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGc                  <  1:458477/149‑1 (MQ=255)                                                                                                                    cAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCat                >  1:626563/1‑149 (MQ=255)                                                                                                                      atatCAAAACGATCAGTGAACAGAAATTTAATGTGATGTCGAAACAGGGCGTCAGATCAGACGAAGGGTTGTTGGAGACCGCCAATATGATGATCTGTATGCGGCATACCGAAACAAGTAATGGGTTGCATCCCTATTAGTCCGCatca              >  2:24633/1‑147 (MQ=255)                                                                                                                      ataaCAAAACGATCCGTGAACATAAATTTAATGTGATGGCGAAACTGGGCGTCAGTTCTGACGCCGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCatga              <  2:550936/149‑1 (MQ=255)                                                                                                                           aaaaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCg         >  1:21070/1‑149 (MQ=255)                                                                                                                             aaCGGTCCGTGAACATAAAGTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGCAATGTGTTGCATCCCTATTAATCCGCATGATGCCggg       <  2:632378/149‑1 (MQ=255)                                                                                                                             aaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCggg       >  1:551795/1‑149 (MQ=255)                                                                                                                                 aTCCGTGAACATAAATTTAATGTGATGTGGGAACTGGGCGTCAGTTGGGACGTGGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTa   <  2:388889/149‑1 (MQ=255)                                                                                                                                 aTCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTa   <  1:270922/149‑1 (MQ=255)                                                                                                                                  cgcGTGAACATAAATTTAATGGGATTGCGAAAATGGGCGTCAGGTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAc  <  2:547995/147‑1 (MQ=255)                                                                                                                                        |                                                                                                                                               TGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAC  >  NC_000913_3_hsa_tpiA/4604563‑4604840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑