New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1843605	43 (1.290)	4 (0.130)	4/262	4.1	8.9%	coding (1235/1344 nt)	gdhA	glutamate dehydrogenase, NADP‑specific
	?	NC_000913_3_hsa_tpiA	= 1843614	42 (1.350)					coding (1244/1344 nt)	gdhA	glutamate dehydrogenase, NADP‑specific
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGTACTATTTGCACCGGGTAAAGCGGCTAATGCTGGTGGCGTCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGC  >  NC_000913_3_hsa_tpiA/1843459‑1843749                                                                                                                                                   |                                                                                                                                                 cGTACTATTTGCACCGGGTAAAGCGGCCAATGCTGGTTTCGTCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCt                                                                                                                                                <  1:545984/149‑1 (MQ=255)                     aaGCGGCTAATGCTGGTGGCGTCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGaa                                                                                                                            <  1:372263/149‑1 (MQ=255)                       gCGGCTAATGCTGGTGGCGTCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCAGCATGCCTGTGTTGAGCGTGGTGGTGAAgg                                                                                                                          >  1:281701/1‑149 (MQ=255)                         ggCTAATGCTGGTGGCGTCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGAATATCCACCATGCCTGTGATGAGCATGGTGGTGAAggag                                                                                                                        >  1:68056/1‑147 (MQ=255)                          gCTAATGCTGGTGGCGTCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGa                                                                                                                       <  2:531902/149‑1 (MQ=255)                                 cTGGTGGCGTCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCCTGCCTGTGTTGAGCATGGGGGTGAAGGTGAGCAAAcc                                                                                                                >  1:275795/1‑149 (MQ=255)                                       gCGTCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTAc                                                                                                          >  2:205972/1‑149 (MQ=255)                                       gCGTCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCAACATGCCTGTGTTGCGAACGTTCGTGAAGGTGAGCAAACCAACTAc                                                                                                          <  2:67911/149‑1 (MQ=255)                                        cGTCGCTACATCGGGCCTGGAAATGGCACCAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCCTGGTGGGGAAGGTGAGCAAACCAACTACg                                                                                                         >  1:55358/1‑149 (MQ=255)                                          tCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGtt                                                                                                                                           <  2:530163/113‑1 (MQ=255)                                          tCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGtt                                                                                                                                           >  1:530163/1‑113 (MQ=255)                                            gCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCa                                                                                                     <  2:498490/149‑1 (MQ=255)                                              tagaTCGGGGCTGGAAATGGCACAAAACGCGGCGGGGCTGGGCAGGAAAGCCGAGAAAGTTGACGCACGTTGGCATCACATCATGGTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAgg                                                                                                   <  2:506976/146‑1 (MQ=255)                                                  tCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGa                                                                                                                       >  2:68364/1‑125 (MQ=255)                                                  tCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGa                                                                                                                       <  1:68364/125‑1 (MQ=255)                                                      gcgtGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAAc                                                                                           <  2:254303/146‑1 (MQ=255)                                                       ccTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACa                                                                                          >  1:463148/1‑149 (MQ=255)                                                          ggAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTg                                                                                       >  2:270741/1‑149 (MQ=255)                                                                 gCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGttt                                                                                >  1:94365/1‑149 (MQ=255)                                                                   acaAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTtg                                                                              >  1:244955/1‑149 (MQ=255)                                                                   acaAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACAATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACGACGTACAGGGCGCGAACATTGCCGGtttgg                                                                              >  1:147035/1‑147 (MQ=255)                                                                    caAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACAGCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTtgt                                                                             <  2:510035/149‑1 (MQ=255)                                                                                  ccTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcgatg                                                               >  1:186758/1‑149 (MQ=255)                                                                                     gggCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCcgatgcga                                                            <  1:270741/149‑1 (MQ=255)                                                                                      ggCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGACGGTTGCcgatgcgat                                                           >  2:105912/1‑149 (MQ=255)                                                                                                 cGAGAAAGTTGACGCACTTTGGCATCACGTCATGCGGGAGATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGTTTGCCGATGCGATGCTGGTGCatg                                                <  1:25227/149‑3 (MQ=255)                                                                                                 cGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCgatgcgatgc                                                         >  1:322679/1‑140 (MQ=255)                                                                                                 cGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCgatgcgatgc                                                         <  2:322679/140‑1 (MQ=255)                                                                                                     aaaGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGgtgt                                            <  2:191811/149‑1 (MQ=255)                                                                                                       agtggccGCAGGTTTGCATCACATCTTGCTGGATATCCACCAGGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACGACGTGGAGGGCGCGGACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGa                                          <  2:214440/143‑1 (MQ=255)                                                                                                        gTTGACGTACGTTTGGATCAGCTCATGGTGGATAGCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGAt                                         <  2:24911/149‑1 (MQ=255)                                                                                                         ttGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGAtt                                        <  2:94365/149‑1 (MQ=255)                                                                                                            aCGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTaa                                     <  2:281701/149‑1 (MQ=255)                                                                                                            aCGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTaa                                     <  2:54962/149‑1 (MQ=255)                                                                                                            aCGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCCCAGGGTGTGATTTaa                                     >  2:478188/1‑149 (MQ=255)                                                                                                                   tttGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTaa                              <  2:463148/149‑1 (MQ=255)                                                                                                                   tttGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTaa                              <  1:413387/149‑1 (MQ=255)                                                                                                                        atcacatcaTGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGAAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTAAAGGTTGGCGTTGTGGTGGTGGCGTAGGGTGTGATTTAAGTTGTATATggc                         >  1:257421/1‑147 (MQ=255)                                                                                                                                aggcgggATATGCTCCAGGCCTGTGGTGAGCACGGTGGAGAAGGGGAGGCAACCGACAACGTGCAGGGCGCGACCATTGTCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATGTAAGTTGTAAAGGCCTGATGgcg                 <  2:354517/144‑1 (MQ=255)                                                                                                                                   gtGGATATCCACCATGCCTGGGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTa              <  2:522865/148‑1 (MQ=255)                                                                                                                                    tGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTCGTAAATGCCTGATGGCGCTAc             <  1:159089/149‑1 (MQ=255)                                                                                                                                       agatCCACCAGGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCt          <  2:289349/147‑1 (MQ=255)                                                                                                                                               cgtGCCTGTGGTGAGCATGGGGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGc  <  2:152276/147‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CGTACTATTTGCACCGGGTAAAGCGGCTAATGCTGGTGGCGTCGCTACATCGGGCCTGGAAATGGCACAAAACGCTGCGCGCCTGGGCTGGAAAGCCGAGAAAGTTGACGCACGTTTGCATCACATCATGCTGGATATCCACCATGCCTGTGTTGAGCATGGTGGTGAAGGTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGC  >  NC_000913_3_hsa_tpiA/1843459‑1843749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.