Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA2,751,9420GC33.3% 30.4 / 10.5 27A50P (GCT→CCT) recNrecombination and repair protein
Reads supporting (aligned to +/- strand):  ref base G (10/8);  new base C (8/1);  total (18/9)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGC  >  NC_000913_3_hsa_tpiA/2751816‑2752088
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aGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTAc                                                                                                                              >  1:395674/1‑149 (MQ=255)
 gCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTTcc                                                                                                                             >  1:124128/1‑149 (MQ=255)
  cAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGGAGCCGACATGGTGCGTACCg                                                                                                                            >  1:255826/1‑149 (MQ=255)
           tATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCCTGTAGTCAACATGATACGTACCGGCTCTGATc                                                                                                                   >  2:316361/1‑149 (MQ=255)
               gttcgtGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCgcgc                                                                                                               >  1:208880/1‑149 (MQ=255)
               gttcgtGAGCTTGAGATTGATTTTCATAGCGGCAAGACCGTAATCTCTGGCGAGCCCGTCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCgcgc                                                                                                               <  2:124128/149‑1 (MQ=255)
                     gAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCt                                                                                                         >  1:548449/1‑149 (MQ=255)
                         ttGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTgc                                                                                                     >  1:448617/1‑149 (MQ=255)
                                     ttCATAGCGGCACGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCAGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtct                                                                                         <  1:316361/149‑1 (MQ=255)
                                      tCACAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtctc                                                                                        <  1:119776/149‑1 (MQ=255)
                                          aGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCATTAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCCTTTTTGTCTGat                                                                                    >  1:73756/1‑148 (MQ=255)
                                               cATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATa                                                                               <  1:194332/149‑1 (MQ=255)
                                               cATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTGACACTGAAAAAAAAAGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGAtc                                                                               >  2:313498/1‑148 (MQ=255)
                                                aTGACCGTAATAACGGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATAc                                                                              <  2:375285/149‑1 (MQ=255)
                                                       taataaCTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCg                                                                       >  1:329988/1‑149 (MQ=255)
                                                       taataaCTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTATCATTAAAAAAAAACGTACCGGCGCTGCTCGCGCTGCCCTGTGCGCCAGTTTTTCTCTGAGAGATACGCCcgcg                                                                       >  2:79082/1‑149 (MQ=255)
                                                                  gagaCCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCCTATCATTAAAAAAAAAAGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCgctg                                                            >  2:402967/1‑149 (MQ=255)
                                                                     aCCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTATCATTAAAAAAAAAAATACCTGCGCCGCTACCGCTGACCTGTCGGACCGTATTACTCTGAAAGATGAGCCAGCGGCTCTgctcgagct                                                         >  2:306702/1‑142 (MQ=255)
                                                                     aCCGGCGCGGGTAAATCTATTCGAAATGTTGCCCTCGGTCTGTGTCTCGGTGGTCGCCCTGAAGCCGACATGGTGCGGCCCGTCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCgctggct                                                         <  2:73756/149‑1 (MQ=255)
                                                                         gcgcGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGgaa                                                     <  1:28361/149‑1 (MQ=255)
                                                                                    tCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTATCATTAAAAAAAAAAATACCGGCGCTGCTCGCGCTGACGTGTGCGACCGTTTTTCTCTAAAACATAAGCCCACGGCTCCTCGCTGGCTCGAAGAAAACCCGCt                                          >  2:470789/1‑149 (MQ=255)
                                                                                    tCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTATAGTTAAAAAAAAAAATAACGGCGCTGCTCGCGCTGACCTGTGAGCCCGTTTTTCTCTGGAAGATACGCCCGCGGCGCTGCGTTGGATGGAATATAACCGGCt                                          >  2:364719/1‑149 (MQ=255)
                                                                                       aTTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGa                                       >  2:485998/1‑149 (MQ=255)
                                                                                           cAATCGATGCCGTCGGTTTTTGTCTCGGGGGTCGGGCTGAAGCCGAGATGGTGCGTACCTGCGCTGCTCGCGCTGACCTGTTCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGAc                                   <  2:259938/149‑1 (MQ=255)
                                                                                           cAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGAc                                   >  1:214849/1‑149 (MQ=255)
                                                                                                 aTGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCAt                             >  1:581848/1‑149 (MQ=255)
                                                                                                          ggcTTTGTCTCGGTGGCGGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTg                    <  2:255826/147‑1 (MQ=255)
                                                                                                                        gggcgcgCTGAAGCGCACAGGGTGCGTACCGGCGCTGCTCGCGCTGACATGTTCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGAt      <  2:282457/146‑1 (MQ=255)
                                                                                                                            gcgcTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATcagc  >  1:480593/1‑149 (MQ=255)
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AGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGC  >  NC_000913_3_hsa_tpiA/2751816‑2752088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑