Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	264,098	0	G	T	100.0%	136.5 / NA	45	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/17);  total (28/17)

CGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCT  >  NC_000913_3_hsa_tpiA/263952‑264226                                                                                                                                                   |                                                                                                                                 cGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGAACAGTTCAACTCGCCACTATTGTCAGTTCAGGGTGATGCTAATCAGGCAGGTATAGGGCTCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAg                                                                                                                                <  1:357768/149‑1 (MQ=38)      tCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTc                                                                                                                           >  1:345655/1‑149 (MQ=255)            gcgcAGTGTAAGCTCCCGCTGGAGGCCCCGGGTAGGGGTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGa                                                                                                                     <  1:232746/149‑1 (MQ=39)                 gtgtAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGCCTTCGGCGATATcc                                                                                                                <  2:385263/149‑1 (MQ=255)                       tctcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGGGGTGCTCATCGGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGACGATATCCGGacgg                                                                                                          >  2:225539/1‑146 (MQ=255)                          cccGCTGCAGGCTCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTcc                                                                                                       <  1:61804/149‑1 (MQ=255)                                gCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCg                                                                                                 <  2:345655/149‑1 (MQ=255)                                  aGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCggg                                                                                               >  1:212365/1‑148 (MQ=255)                                   ggCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                                                                                              <  1:704903/149‑1 (MQ=255)                                   ggCCCCCGGTAGGGGTGCTCATTACAGTTCTCCACTAGTGTCAGTGCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtg                                                                                              <  1:340270/149‑1 (MQ=255)                                             aGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGa                                                                                    <  2:441278/149‑1 (MQ=255)                                               ggTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGGCTCCGGCGATATCCGGAACGTCCCTGCCGGTGGAATGCCAGACg                                                                                  >  1:584167/1‑149 (MQ=255)                                               ggTTGCTCATTACAGTTCTCCACTATTGTCAGCATTATGAGAGGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACg                                                                                  >  1:720627/1‑149 (MQ=25)                                                 ttGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCt                                                                                >  1:383379/1‑149 (MQ=255)                                                    cTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTAcc                                                                             >  1:371743/1‑149 (MQ=255)                                                       aTTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGGTACCGTg                                                                          >  1:155838/1‑149 (MQ=255)                                                        ttACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGc                                                                         <  2:406343/149‑1 (MQ=255)                                                        ttACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCAGGTGTAATGACAGACGCTACCGTGc                                                                         >  1:6720/1‑149 (MQ=255)                                                              ttCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCGATTGCGGTCCTGGCGGCGTTCGGCGTCTACCGCTAGGACTCCGGCGATATCCGGAACGTCACTGCCGGAGAAATGACAGACTCTACCGTGCCAcgag                                                                   >  2:391695/1‑146 (MQ=21)                                                                 tcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGGAATGACAGACGCTACCGTGCCACTGGTAt                                                                >  1:626933/1‑149 (MQ=255)                                                                  ccACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGGGGGCGTGGCGGCGTTCGGAGTTTTCCGCTAGGACTCCGGCGATATCTGGAAGGTGCCTGCCGGTATCACGACAGCGGCTACCCTGCCCCTGGTCtt                                                               >  2:20453/1‑149 (MQ=21)                                                                  ccACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTACCGGTGTCATGACAGACGCTACCGTGCCATTGGTAtt                                                               >  2:245096/1‑149 (MQ=255)                                                                   cACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGACCGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAttt                                                              >  1:305585/1‑149 (MQ=255)                                                                            cAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCa                                                     <  2:383379/149‑1 (MQ=255)                                                                               ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTa                                                  <  1:663366/149‑1 (MQ=255)                                                                                    ggTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGCACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGaa                                             >  2:254150/1‑149 (MQ=255)                                                                                          gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTGGGACTCCGTCGATATCCTGAACGTCCCTGCCGGGGTAATGACAAACACTACCGTGCCACAGGGATTTGCCGGTGCAGTAGCGAAAGAtgc                                       >  1:373005/1‑147 (MQ=21)                                                                                           cgcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCg                                      <  1:173054/147‑1 (MQ=255)                                                                                            tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCAc                                                                      >  2:35602/1‑116 (MQ=255)                                                                                            tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCAc                                                                      <  1:35602/116‑1 (MQ=255)                                                                                              atcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGCTATCCGGAACGTGCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGa                                   >  1:66981/1‑149 (MQ=255)                                                                                              atcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGa                                   >  1:244860/1‑149 (MQ=255)                                                                                                   gcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAg                              >  1:664612/1‑149 (MQ=255)                                                                                                   gcaggTATAGGGCCCATTGCGGTCCTGACTGCGCTCGGCCTATACCGCTAGGACTCCGGCGATATCCGGCACGTCCCTGCCGGTGTAATGACCGGAGCTACCGTGCCACTGGACTTTACCGGTGCAGTAGCGAAAGATTCGGGACTCAg                              >  2:670543/1‑149 (MQ=18)                                                                                                     aggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAGGATTCCGGACTCGGGa                            >  1:163182/1‑149 (MQ=255)                                                                                                       gTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATg                          >  2:115585/1‑149 (MQ=255)                                                                                                        tataGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGc                         >  1:634781/1‑149 (MQ=255)                                                                                                             ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCg                    <  1:527981/149‑1 (MQ=255)                                                                                                             ggCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCg                    >  1:161797/1‑149 (MQ=255)                                                                                                               cccATTGCGGTCCTAGCGGCGTTCTGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGt                  >  2:313183/1‑149 (MQ=255)                                                                                                                   ttccGGTCTTTGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTatat              <  2:584167/146‑1 (MQ=255)                                                                                                                         tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTcct        >  1:627352/1‑147 (MQ=255)                                                                                                                           cTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTTTAATGACAGACGCTGCCGTGCCACTGGTATTTGGCGGTGCAGTAGCAGAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCTTTg      >  2:56881/1‑149 (MQ=17)                                                                                                                              gcggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGccc   <  2:295487/149‑1 (MQ=255)                                                                                                                               cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCAGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCt  <  1:514473/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                 CGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCT  >  NC_000913_3_hsa_tpiA/263952‑264226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑