Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_hsa_tpiA	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_hsa_tpiA	1978503	1979278	776	50 [0]	[0] 50	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 1978502	0 (0.000)	49 (0.930)	44/264	0.2	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_hsa_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

AAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC  >  NC_000913_3_hsa_tpiA/1978357‑1978644                                                                                                                                                  |                                                                                                                                               aaaTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                                                             <  1:926107‑M1/149‑4 (MQ=255)       gTCTTTCCACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATGCAACGGAGGTGGGCTGCGATTTTCAATGATTCGTGATGCCGATCCCACAAAACAATCAATTGCTTGACTCAAAAGtataaatca                                                                                                                                       >  1:433144‑M1/1‑140 (MQ=255)        tCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcat                                                                                                                                      <  2:799882‑M1/149‑11 (MQ=255)           ttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATAACACAAAACACTCAATTACTTAACATAAATGtctaattcatatt                                                                                                                                   >  2:577315‑M1/1‑136 (MQ=255)                 ttATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCACTTACTTAACATAAATGtataagtcatacttttgtt                                                                                                                             >  2:249913‑M1/1‑130 (MQ=255)                 ttATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                                                             >  1:977379‑M1/1‑130 (MQ=255)                 ttATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                                                             <  2:977379‑M1/133‑4 (MQ=255)                 ttATCGAAGATTTATCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtt                                                                                                                             <  1:349105‑M1/149‑20 (MQ=255)                  tATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttggttt                                                                                                                            <  2:507695‑M1/149‑21 (MQ=255)                            ttttCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatt                                                                                                                  <  1:737776‑M1/149‑31 (MQ=255)                                 cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatgcttttgttttgggtgtatttcaat                                                                                                             >  1:331949‑M1/1‑114 (MQ=255)                                   aaaTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatct                                                                                                           <  1:349585‑M1/149‑38 (MQ=255)                                     aTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATGACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                                                                         <  1:788528‑M1/149‑40 (MQ=255)                                        gACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttag                                                                                                      >  1:855358‑M1/1‑107 (MQ=255)                                           gCAACTGTACTCGTCACTACACGCACATCCAACGGAGGGCGGCTGCGATTTTCAATCATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgctttggctggctttcaatctgttaaaaa                                                                                                   >  1:641291‑M1/1‑104 (MQ=255)                                           gCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaa                                                                                                   <  1:897938‑M1/149‑46 (MQ=255)                                             aaCTATACTCGTCACTACACGCACATACAACGGGGGGGGGCGGCGATGTTCAATAATGCGTGATGCAGATCACACAAAACGATCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagt                                                                                                 >  2:18494‑M1/1‑102 (MQ=255)                                               cTGCACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttt                                                                                               <  1:671922‑M1/149‑50 (MQ=255)                                                 cgacgcgtcacTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttt                                                                                             <  2:494044‑M1/144‑52 (MQ=255)                                                  tACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                                                            >  1:50878‑M1/1‑97 (MQ=255)                                                     tCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgct                                                                                         >  1:148400‑M1/1‑94 (MQ=255)                                                                   acaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaa                                                                           >  1:791109‑M1/1‑80 (MQ=255)                                                                       aCAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaat                                                                       >  1:562254‑M1/1‑76 (MQ=255)                                                                           cGGGGGGGGTCTGCGATTTTCAATAACGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                                   <  1:927254‑M1/149‑78 (MQ=255)                                                                              aGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacag                                                                >  2:586072‑M1/1‑69 (MQ=255)                                                                               ggggggCTGCGATTTTCAAAAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctcttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagg                                                               <  2:148400‑M1/149‑82 (MQ=255)                                                                                gggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagga                                                              <  2:438530‑M1/149‑83 (MQ=255)                                                                                    gCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataa                                                          <  2:50878‑M1/149‑87 (MQ=255)                                                                                     cTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataat                                                         >  2:353958‑M1/1‑62 (MQ=255)                                                                                      tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc                                                        >  2:409339‑M1/1‑61 (MQ=255)                                                                                        cGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcga                                                      <  1:586072‑M1/149‑91 (MQ=255)                                                                                         gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaaaatgaataatcgaa                                                     >  2:279295‑M1/1‑58 (MQ=255)                                                                                           ttttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaat                                                   <  2:269768‑M1/149‑94 (MQ=255)                                                                                            tttCAAAAATGCGTGATGCAGATCACACAAAACACTCCATTACGTAACATAAATGtataagtcatacttttgttttgggggtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatg                                                  <  1:763451‑M1/149‑95 (MQ=255)                                                                                                aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggat                                              >  1:665683‑M1/1‑51 (MQ=255)                                                                                                   aatGCCTGATGCACATCACACAAAATACTCAATTACGTAACATAAATGtataagtcatacttttgttttgggtgcatttcaatctgttaaaaagtttttcgctacgctatcaagcaaaaatgctgcaggaagaatcgaaatgggatgtt                                           <  2:362707‑M1/149‑102 (MQ=255)                                                                                                            tGCAGATCACACAAAACACTCAATTCCTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaacaaggttttcgctacgctagcacgccaaaatgaaacaggaataatcgaaatgggatgttgcgcacagt                                  <  1:683716‑M1/149‑111 (MQ=255)                                                                                                            tGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagaaaaaatgaaacaggaataatcgaaatgggatgttccgcacagc                                  >  2:199279‑M1/1‑39 (MQ=255)                                                                                                                 aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaa                             >  2:82539‑M1/1‑34 (MQ=255)                                                                                                                    acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaacataa                          >  1:968045‑M1/1‑31 (MQ=255)                                                                                                                      acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc                                                        <  1:675004‑M1/117‑89 (MQ=255)                                                                                                                      acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc                                                        >  2:675004‑M1/1‑29 (MQ=255)                                                                                                                          aaCAATCCATTCCTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcacc                    <  2:191387‑M1/149‑125 (MQ=255)                                                                                                                              cTCAATTACTTAACATAAATGtataagtcatacttttgtttttggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtccaaataacacaccgtaa                >  2:525907‑M1/1‑21 (MQ=255)                                                                                                                               tCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaa               >  2:109237‑M1/1‑20 (MQ=255)                                                                                                                                 aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc                                                        <  2:357883‑M1/106‑89 (MQ=255)                                                                                                                                 aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc                                                        >  1:357883‑M1/1‑18 (MQ=255)                                                                                                                                      cgtgacataGATGgataagttatacttttgttttgggtgtatgtcaatctgtaaaaaaggttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcat        <  2:641291‑M1/145‑137 (MQ=255)                                                                                                                                      attaACATTAATGtataaggcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcag        <  1:199279‑M1/148‑137 (MQ=255)                                                                                                                                            aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgcta  >  1:171210‑M1/1‑7 (MQ=255)                                                                                                                                                  |                                                                                                                                               AAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGC  >  NC_000913_3_hsa_tpiA/1978357‑1978644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑