New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	154541 =	63 (1.040)	6 (0.110)	6/266	7.2	9.3%	coding (886/2598 nt)	htrE	putative outer membrane usher protein
	?	NC_000913_3_hsa_tpiA	154564 =	57 (1.000)					coding (863/2598 nt)	htrE	putative outer membrane usher protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGA  >  NC_000913_3_hsa_tpiA/154416‑154703                                                                                                                                                     |                                                                                                                                            ggATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCGACGCCat                                                                                                                                             <  2:838426/149‑1 (MQ=255) ggATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCat                                                                                                                                             <  2:408550/149‑1 (MQ=255)    ttcttcGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCatgaa                                                                                                                                          <  2:149763/149‑1 (MQ=255)       ttcGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCatgaatga                                                                                                                                       >  1:957821/1‑149 (MQ=255)        tcGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGAt                                                                                                                                      >  2:534927/1‑149 (MQ=255)                       aGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGc                                                                                                                       >  1:589902/1‑149 (MQ=255)                        gATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATCGTGACGTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCt                                                                                                                      >  1:244578/1‑149 (MQ=255)                         aTCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTa                                                                                                                     >  1:874747/1‑149 (MQ=255)                             cTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGGGACTTTGGCGTTGGTATTGGCACCGCCATGAATGATAGGCGCAAAGCTGGATAAGGt                                                                                                                 >  1:283775/1‑149 (MQ=255)                                ccGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGAt                                                                                                                                      >  2:883001/1‑125 (MQ=255)                                ccGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGAt                                                                                                                                      <  1:883001/125‑1 (MQ=255)                                ccGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCgg                                                                                                              <  1:751808/149‑1 (MQ=255)                                 cGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGa                                                                                                             <  1:635498/149‑1 (MQ=255)                                 cGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGa                                                                                                             <  1:647749/149‑1 (MQ=255)                                      ctggACGGCGTCTGATCATCAATGTCGAACGCGCCTGGCGGCACCGTCGTTTCATAATTCGTATAGCCACCTTGCGTAATAGTTACTTTGGCGTTGGTATTGGCTACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCaa                                                                                                        <  2:475368/146‑1 (MQ=255)                                      ccTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCaa                                                                                                        >  1:852334/1‑149 (MQ=255)                                        tGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACa                                                                                                      <  1:534927/149‑1 (MQ=255)                                           cGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGc                                                                                                   <  2:852334/149‑1 (MQ=255)                                               cTCAGATCATCAATGACGAACGCGCCTGGCGGCACCGTCGTTTCATCAGTGTCATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCt                                                                                               <  1:670571/149‑1 (MQ=255)                                                  aGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTc                                                                                            >  2:213106/1‑149 (MQ=255)                                                   gATCATCAAGGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCa                                                                                           <  2:79684/149‑1 (MQ=255)                                                    atcatcAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCAc                                                                                          <  2:799684/149‑1 (MQ=255)                                                     tcatcaATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACt                                                                                         <  2:24224/149‑1 (MQ=255)                                                      catcaATGACGAAAGCGCCTGGCTGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTg                                                                                        <  1:287150/149‑1 (MQ=255)                                                       atcaATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCg                                                                                                               >  2:1031895/1‑125 (MQ=255)                                                       atcaATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCg                                                                                                               <  1:1031895/125‑1 (MQ=255)                                                             gACGAAAGCGCGTGGCGGCAGCGTCGGTTCATAAATCTTATAGCCAACTTGTGTAGTAGTGACTTTGGCGTTGGTATTGGCAACGTCATGAATGATGGTCGCAAAGCTGGCTAAAGTCGTAGGCAACATGCGGCTGTCACTGTATAAAc                                                                                 <  2:151312/149‑1 (MQ=255)                                                                  aaGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATg                                                                            <  1:1049099/149‑1 (MQ=255)                                                                  aaGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACCGTATAAACGAATg                                                                            >  2:260827/1‑149 (MQ=255)                                                                        cTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATGGTGACTTTGGCGTTGGTATTGGCCACGCCATGACTGATAGGCGCAAAGCTGGCTCAAGTCGGAGGCAACATGGGGCTGTAACTGTATAAAAGAATGCCTCgg                                                                      >  1:482319/1‑149 (MQ=255)                                                                         tGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGa                                                                     <  2:874747/149‑1 (MQ=255)                                                                         tGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGa                                                                     <  1:213106/149‑1 (MQ=255)                                                                         gggcggcACCGGCGTTTCATAAATCTTATAGCCACCGTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGa                                                                     <  2:137210/148‑1 (MQ=255)                                                                                aCCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGAc                                                              <  2:870737/149‑1 (MQ=255)                                                                                aCCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGAc                                                              <  2:841537/149‑1 (MQ=255)                                                                                aCCGTCGTTTCATAAATCTTAGAGCCGCCTTGCGTAATAGTGAATTTGGCGTTGGGATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTCTAAACGCATGCCCCGGATACTTAc                                                              <  2:656197/149‑1 (MQ=255)                                                                                           aTAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGCCGGAATCAAAgg                                                   >  2:56103/1‑149 (MQ=255)                                                                                              aaTCTTATAGCCACCTTGCGTAATAGTGAGTTTGGCGTCGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAATGttt                                                >  1:893394/1‑149 (MQ=255)                                                                                              aaTCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGttt                                                >  2:138330/1‑149 (MQ=255)                                                                                                tCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCg                                              >  1:842043/1‑149 (MQ=255)                                                                                                 cTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGc                                             <  2:980435/149‑1 (MQ=255)                                                                                                                  tAATAGGTACTTTGGCGGTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCa                            <  2:788707/149‑1 (MQ=255)                                                                                                                    aTAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGc                                                                                                          >  2:78046/1‑69 (MQ=255)                                                                                                                    aTAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGc                                                                                                          <  1:78046/69‑1 (MQ=255)                                                                                                                      aGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCaa                        <  1:559912/149‑1 (MQ=255)                                                                                                                        tGACTTTGGGGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGa                      <  2:618628/149‑1 (MQ=255)                                                                                                                          accttGGCGGTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAAt                    <  1:987568/146‑1 (MQ=255)                                                                                                                                gCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCCACAATTAGTTg              <  2:277421/149‑1 (MQ=255)                                                                                                                                  gTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTgag            >  1:850742/1‑149 (MQ=255)                                                                                                                                    tGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGAc                                                              <  2:283900/97‑1 (MQ=255)                                                                                                                                    tGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGAc                                                              >  1:283900/1‑97 (MQ=255)                                                                                                                                      gTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACg        >  2:169849/1‑149 (MQ=255)                                                                                                                                            gCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGt                                                  <  1:968803/101‑1 (MQ=255)                                                                                                                                            gCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGt                                                  >  2:968803/1‑101 (MQ=255)                                                                                                                                            gCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCaa                        <  2:735607/127‑1 (MQ=255)                                                                                                                                            gCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCaa                        >  1:735607/1‑127 (MQ=255)                                                                                                                                            gCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGa  <  1:56103/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                            GGATTCTTCGATGGTAACAATAAGATCGCTGCCGTACCCTGACGGACTCAGATCATCAATGACGAAAGCGCCTGGCGGCACCGTCGTTTCATAAATCTTATAGCCACCTTGCGTAATAGTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGA  >  NC_000913_3_hsa_tpiA/154416‑154703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.