Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,088,188 G→A intergenic (+328/‑96) metK → / → galP S‑adenosylmethionine synthetase/D‑galactose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,088,1880GA100.0% 308.9 / NA 94intergenic (+328/‑96)metK/galPS‑adenosylmethionine synthetase/D‑galactose transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/54);  total (40/54)

TAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTT  >  NC_000913/3088043‑3088333
                                                                                                                                                 |                                                                                                                                                 
tAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATaa                                                                                                                                                >  1:705240/1‑149 (MQ=255)
tAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATaa                                                                                                                                                >  2:242981/1‑149 (MQ=255)
tAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTAAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATaa                                                                                                                                                >  2:211520/1‑149 (MQ=255)
tAATATTATCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTATTCAGTAAAGTCTTAATTGCAGATAACAGCGTTAAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGATAGTATaa                                                                                                                                                >  2:704377/1‑149 (MQ=255)
     ttttCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATATATGATGATATAACTCAATTATTTTCATGCAATTAAATCATAACTAAGATAAATGTTAGTATAAGCGAt                                                                                                                                           >  1:876148/1‑149 (MQ=255)
     ttttCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAACGCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGAt                                                                                                                                           <  2:1045459/149‑1 (MQ=255)
      tttCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGAtt                                                                                                                                          >  1:266250/1‑149 (MQ=255)
      tttCATATTATTATTCGGTTTTCACAGTTGTTACAGTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATTATGATAGAACTCAATTATTTTAATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGAtt                                                                                                                                          >  2:1109593/1‑149 (MQ=255)
         cATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTaca                                                                                                                                       <  2:277461/149‑1 (MQ=255)
         aatatTATTATTCGGTTTTCACATTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTaca                                                                                                                                       <  1:1132083/148‑1 (MQ=255)
         aatatTATTATTCGGTTTTCACATTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTaca                                                                                                                                       <  1:1127741/148‑1 (MQ=255)
          atatTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTacac                                                                                                                                      <  2:363852/149‑1 (MQ=255)
          aaattattattCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTacac                                                                                                                                      >  1:467762/3‑149 (MQ=255)
           tattattatTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACt                                                                                                                                     <  2:241324/149‑1 (MQ=255)
              tattattCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGAt                                                                                                                                  >  1:219063/1‑149 (MQ=255)
              tattattCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGAt                                                                                                                                  <  2:1092720/149‑1 (MQ=255)
                ttattCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGAtgt                                                                                                                                <  1:713407/149‑1 (MQ=255)
                  attCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGAGATTACACTGATGTGa                                                                                                                              >  2:727195/1‑149 (MQ=255)
                   ttCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAGATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATTTGAt                                                                                                                             >  1:704224/1‑149 (MQ=255)
                    tCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGAt                                                                                                                                  >  1:338041/1‑143 (MQ=255)
                    tCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGAt                                                                                                                                  <  2:338041/143‑1 (MQ=255)
                       gTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGc                                                                                                                         <  1:1120567/149‑1 (MQ=255)
                       gTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGc                                                                                                                         <  2:266250/149‑1 (MQ=255)
                       gTTTTCACAGGTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGc                                                                                                                         <  2:754304/149‑1 (MQ=255)
                        ttttCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCt                                                                                                                        <  2:705240/149‑1 (MQ=255)
                         tttCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCtt                                                                                                                       >  1:26265/1‑149 (MQ=255)
                         tttCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCtt                                                                                                                       <  2:209929/149‑1 (MQ=255)
                         tttCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCtt                                                                                                                       >  2:380614/1‑149 (MQ=255)
                           tCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTca                                                                                                                     <  2:231550/149‑1 (MQ=255)
                                gttgttACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCt                                                                                                                >  2:244595/1‑149 (MQ=255)
                                  tgttACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCttt                                                                                                              <  1:1012940/149‑1 (MQ=255)
                                    ttACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCttttt                                                                                                            <  1:727195/149‑1 (MQ=255)
                                     tACATTTCTTTTCAGTAAAGGCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTa                                                                                                           <  2:1038567/149‑1 (MQ=255)
                                      aCATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTAc                                                                                                          <  1:349704/149‑1 (MQ=255)
                                        aTTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATTATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTAcgt                                                                                                        >  2:826310/1‑149 (MQ=255)
                                         tttCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTAcgtc                                                                                                       <  1:234673/149‑1 (MQ=255)
                                            cTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTa                                                                                                    <  1:1113647/149‑1 (MQ=255)
                                               ttCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTTTGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTAGTACTc                                                                                                 >  1:166406/1‑149 (MQ=255)
                                                tCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCa                                                                                                <  2:219063/149‑1 (MQ=255)
                                                   gTAAAGTCTTAATTGCAGATAACAGCTTTTAATCTATGATGATATTACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCt                                                                                             <  1:669532/149‑1 (MQ=255)
                                                   gTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCt                                                                                             <  1:1172505/149‑1 (MQ=255)
                                                    tAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAATATAAAAGATAGTATAAGCGATTACACTGATGTGATTAGCTTCACATCTTTTTAAGTCGTACTCACCTa                                                                                            >  2:1098703/1‑149 (MQ=255)
                                                      aaGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATc                                                                                          <  1:215766/149‑1 (MQ=255)
                                                       aGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCt                                                                                         >  2:164825/1‑149 (MQ=255)
                                                          cTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTaa                                                                                      <  2:482833/149‑1 (MQ=255)
                                                           ttAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAt                                                                                     >  2:1162645/1‑149 (MQ=255)
                                                           ttAATTGCAGATAACAGCGTTTAATATATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAt                                                                                     <  1:661719/149‑1 (MQ=255)
                                                           atAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGAATACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAt                                                                                     <  1:849274/148‑1 (MQ=255)
                                                            tAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATACGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAtt                                                                                    <  1:208350/149‑1 (MQ=255)
                                                            tAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAtt                                                                                    <  2:980216/149‑1 (MQ=255)
                                                              aTTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTca                                                                                  <  1:1111203/149‑1 (MQ=255)
                                                               ttGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTcac                                                                                 <  2:467762/149‑1 (MQ=255)
                                                               ttGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTcac                                                                                 <  2:499507/149‑1 (MQ=255)
                                                               ttGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTcac                                                                                 >  2:546272/1‑149 (MQ=255)
                                                               atGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACATAAATCATAACTAAGATAAATGTTACTATAACCGATTACACTGATCTGATTTGCTTCACATCATTTTACGTCTTACACACATAACTTAATTcta                                                                                 >  1:310883/2‑147 (MQ=255)
                                                                    gATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATaa                                                                            <  2:644622/149‑1 (MQ=255)
                                                                      tAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATaaaa                                                                          <  2:813935/149‑1 (MQ=255)
                                                                       aaCAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATaaaaa                                                                         >  2:172275/1‑149 (MQ=255)
                                                                        aCAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATaaaaaa                                                                        <  1:520005/149‑1 (MQ=255)
                                                                          aGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAATTGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATa                                                                      >  1:922836/1‑149 (MQ=255)
                                                                          aGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATa                                                                      <  1:987654/149‑1 (MQ=255)
                                                                           gCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATaa                                                                     <  2:582956/149‑1 (MQ=255)
                                                                           gCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATaa                                                                     <  1:42902/149‑1 (MQ=255)
                                                                            ggTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAAc                                                                    <  1:30572/148‑1 (MQ=255)
                                                                            cGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAAc                                                                    >  2:708740/1‑149 (MQ=255)
                                                                               ttAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCat                                                                 >  2:274438/1‑149 (MQ=255)
                                                                                   tCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTg                                                             >  2:598361/1‑149 (MQ=255)
                                                                                     tATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGa                                                           >  2:385692/1‑149 (MQ=255)
                                                                                       tgatgaTATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCCCCTATCTTAATTCACAATAAAAAATAACCATATTGGAgg                                                         >  1:686867/1‑149 (MQ=255)
                                                                                       tgatgaTATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAgg                                                         <  1:241107/149‑1 (MQ=255)
                                                                                       tgatgaTATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAgg                                                         >  2:392475/1‑149 (MQ=255)
                                                                                        tattatatAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAggg                                                        <  2:927459/145‑1 (MQ=255)
                                                                                        gatgatATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTTTATTAATTCACAATAAAAAATAACCATATTTGAgtg                                                        >  2:612077/1‑147 (MQ=255)
                                                                                          tgatATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGca                                                      >  2:77657/1‑149 (MQ=255)
                                                                                           tatatAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGcat                                                     <  1:326036/148‑1 (MQ=255)
                                                                                                aaCTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGc                                                >  1:1097926/1‑149 (MQ=255)
                                                                                                  cTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCt                                              <  1:502277/149‑1 (MQ=255)
                                                                                                           tttCATGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTaaa                                     >  2:423741/1‑149 (MQ=255)
                                                                                                               attcACTTAAATCATAACTAAGATAAATTTTATTATTAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCTTACTCACCTATCTTAATTCACAATTAAAAATAACCATATTGGAGTGCATCATTCCTGACGCTAAAAAAc                                 <  1:809994/146‑1 (MQ=255)
                                                                                                               aTGCACTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAAc                                 >  1:860724/1‑149 (MQ=255)
                                                                                                                   aCTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAggg                             <  1:252363/149‑1 (MQ=255)
                                                                                                                   aCTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAggg                             >  1:863500/1‑149 (MQ=255)
                                                                                                                   aCTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAggg                             <  1:90914/149‑1 (MQ=255)
                                                                                                                    cTTAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGCTGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAgggg                            <  1:107005/149‑1 (MQ=255)
                                                                                                                     ttAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGc                           >  1:362289/1‑149 (MQ=255)
                                                                                                                     ttAAATCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGc                           >  2:476408/1‑149 (MQ=255)
                                                                                                                       aaaTCATAACTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCgg                         <  1:598361/149‑1 (MQ=255)
                                                                                                                                cTAAGATAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAg                <  1:211520/149‑1 (MQ=255)
                                                                                                                                     aTAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAAt           <  2:860724/149‑1 (MQ=255)
                                                                                                                                      tAAATGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATg          >  1:763012/1‑149 (MQ=255)
                                                                                                                                       aaaTGTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGa         <  1:423741/149‑1 (MQ=255)
                                                                                                                                        aaagTTAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGGCAAACAAGGCAATGAc        <  2:1150877/146‑1 (MQ=255)
                                                                                                                                            ttAGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGttt    <  1:380614/149‑1 (MQ=255)
                                                                                                                                              aGTATAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGAGCATAATGCCTGACGCTAAAAAACAGGGGCGGTCAAACATGCCAATGACGttttt  >  2:555808/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTT  >  NC_000913/3088043‑3088333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: