Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,088,193	C→A	intergenic (+333/‑91)	metK → / → galP	S‑adenosylmethionine synthetase/D‑galactose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,088,193	0	C	A	100.0%	155.4 / NA	45	intergenic (+333/‑91)	metK/galP	S‑adenosylmethionine synthetase/D‑galactose transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/22);  total (23/22)

ATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTTCG  >  NC_000913/3088047‑3088335                                                                                                                                                   |                                                                                                                                               atTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAgaga                                                                                                                                              <  1:833477/149‑1 (MQ=255)    ttCATATTATTATTCGGTTTTCATAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATCTAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTa                                                                                                                                           <  1:727712/149‑1 (MQ=255)      cATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTaca                                                                                                                                         <  2:688184/149‑1 (MQ=255)       atatTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTacac                                                                                                                                        <  2:751353/149‑1 (MQ=255)         attattattCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATTTTATTGTAAGAGATTACACTg                                                                                                                                      >  2:421619/1‑149 (MQ=255)           tattatgCCGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGAt                                                                                                                                    <  1:204207/149‑1 (MQ=255)                    gTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGAtt                                                                                                                              <  1:434457/146‑1 (MQ=255)                    gTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGAtt                                                                                                                              >  2:434457/1‑146 (MQ=255)                       ttaacaGTTGTTACTATTATTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCACGCACTTAAATCATAACTACGATATATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTc                                                                                                                        <  2:617901/146‑1 (MQ=255)                             gttgttACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCt                                                                                                                  >  2:37438/1‑149 (MQ=255)                               ttttACATTTCTTTTCAGTAAAGTCTGAATTGCAGATAACAGTGTTTACTCTAAGAGGATATAACCCATTTATTTTCATGCACTTAAATCTTAACTAAGATAAAGGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCttt                                                                                                                <  1:597397/147‑1 (MQ=255)                                gttACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACTTCtttt                                                                                                               >  1:233284/1‑149 (MQ=255)                                 ttACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCttttt                                                                                                              >  2:348584/1‑149 (MQ=255)                                    cATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTAcg                                                                                                           >  1:567992/1‑149 (MQ=255)                                       ttCTTTTCAGTAAAGTCTTAATTGCGGATAACAGCGTTTAATCTATAATGATATAACTCAATTATTAGCATGCACTTAAATCATGACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTCATTTGCTTCACATCTTTTAAcgtcg                                                                                                        >  1:779311/1‑149 (MQ=255)                                         cTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTa                                                                                                      <  1:37438/149‑1 (MQ=255)                                          ttttCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTAc                                                                                                     >  2:470025/1‑149 (MQ=255)                                          ttttCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGAAc                                                                                                     >  2:178633/1‑149 (MQ=255)                                               aGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCAcc                                                                                                >  1:79476/1‑149 (MQ=255)                                                    aGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCt                                                                                           <  1:699545/149‑1 (MQ=255)                                                         tAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAtt                                                                                      <  2:80518/149‑1 (MQ=255)                                                                     aCAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATaaaaaa                                                                          <  1:857336/149‑1 (MQ=255)                                                                       aGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACACTAAAAAATa                                                                        >  1:831897/1‑149 (MQ=255)                                                                       aGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATa                                                                        <  1:178633/149‑1 (MQ=255)                                                                         cGTTTAATCTATGCTGATATAACTCAATTATCTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAAc                                                                      <  1:139722/149‑1 (MQ=255)                                                                           tttAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCa                                                                    >  1:62577/1‑149 (MQ=255)                                                                                   atgatgATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAg                                                            >  1:152666/1‑149 (MQ=255)                                                                                    tgatgaTATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAgg                                                           <  2:811297/149‑1 (MQ=255)                                                                                    tgatgaTATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAgg                                                           <  1:43128/149‑1 (MQ=255)                                                                                    tgatgaTATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAgg                                                           <  2:62577/149‑1 (MQ=255)                                                                                    tgatgaTATAACTCAATTATTTTCATGAACTTAAATCATAACTAATATAAATGTTAGTGTAAGAGATTACACTGATGTGACTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAgg                                                           <  1:360583/149‑1 (MQ=255)                                                                                     gatgatATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAgtg                                                          >  2:293765/1‑147 (MQ=255)                                                                                     gatgatATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAggg                                                          >  2:444098/1‑149 (MQ=255)                                                                                          tataACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGcatca                                                     <  1:621632/149‑1 (MQ=255)                                                                                          tataACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGcatca                                                     <  1:470025/149‑1 (MQ=255)                                                                                             aaCTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCTCCTCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGc                                                  <  2:709512/149‑1 (MQ=255)                                                                                              aCTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGcc                                                 <  2:444166/149‑1 (MQ=255)                                                                                                  aaTTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGAc                                             >  1:582628/1‑149 (MQ=255)                                                                                                       ttttCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTaa                                        >  1:757373/1‑149 (MQ=255)                                                                                                          tCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTaaaaa                                     >  2:701572/1‑149 (MQ=255)                                                                                                                       tCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCa                        >  2:681806/1‑149 (MQ=255)                                                                                                                          tAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAAc                     >  2:262806/1‑149 (MQ=255)                                                                                                                            aCTAAGATAAATGTTAGTGTAAGACATGACATTGATTGGATTTGCTTCACAGCTTTTTACGGCGTACGTACCTATCTTAATTCACACTGAAGTATAACCGGATTGGAGCGCTTCAGGCCTGACGCTAAAAAACAGTGGCGGTCAAACaa                   <  2:533932/149‑1 (MQ=255)                                                                                                                                 gATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCaa              >  2:806267/1‑149 (MQ=255)                                                                                                                                        gTTAGTGGAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGTCGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGtt       >  2:494662/1‑149 (MQ=255)                                                                                                                                             tgtAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGTGGCGGTCAGAAAAGGCAATGACGTTTTTCg  >  2:570052/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                               ATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTTCG  >  NC_000913/3088047‑3088335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑