Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258674 258683 10–776 111 [0] [1] 114 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)103 (0.960) 72/280 0.4 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 114 total aligned reads displayed.

CTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTC  >  NC_000913/258537‑258832
                                                                                                                                                   |                                                                                                                                                    
gcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                                                                     <  1:540077‑M2/2‑1 (MQ=255)
  ttttgttaaaaatttgctaaaatttgccaatatggtaaaacagttgcatcacaacaggagatagcaatgccgttaccgagtggacacccgaagagcagaatgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTg                                                                                                                                                   >  2:1327019‑M2/146‑149 (MQ=255)
   tgtgttaaaactttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGc                                                                                                                                                  <  1:104976‑M2/5‑1 (MQ=255)
     tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaa                                                                                                                                                <  1:796563‑M2/7‑1 (MQ=255)
     tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaa                                                                                                                                                <  1:378097‑M2/7‑1 (MQ=255)
     tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaa                                                                                                                                                <  1:1360746‑M2/7‑1 (MQ=255)
      gttaaaaatttgctaaattttgccaatttggtaaatcagttgcatcacaacaggagaaagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaaa                                                                                                                                               <  1:1567218‑M2/8‑1 (MQ=255)
      gttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaaa                                                                                                                                               <  2:1276605‑M2/8‑1 (MQ=255)
      gttaaaaatttgctaaattttgccaatttggtaaaacaggtgcatcacaactggagatagcaatgacgttaccgagtggacacccgaagagctgattgatcaaaaaatttaccgcactaggcccggatattcgtgaagctaAGTGCaaa                                                                                                                                               >  1:201748‑M2/142‑149 (MQ=255)
         aaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgatgagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGAt                                                                                                                                            >  1:463892‑M2/139‑149 (MQ=255)
           aaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATaa                                                                                                                                          >  2:648564‑M2/137‑149 (MQ=255)
             atttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                                                                        <  2:648972‑M2/15‑1 (MQ=255)
             atttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                                                                        <  2:509280‑M2/15‑1 (MQ=255)
                 gctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAtt                                                                                                                                    <  1:778761‑M2/19‑1 (MQ=255)
                  ctaaatttttccaatttggtaaaactgttgcatgactacaggtgatagctatgactttaccgagtggaaacgcgaagatcatattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGTTTc                                                                                                                                   <  1:1648860‑M2/20‑1 (MQ=255)
                  ctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttacagcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                                                                   >  1:1261883‑M2/130‑149 (MQ=255)
                   taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggactcccgaagagcagattgatcaaaaaatttacctcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                                                                  >  2:326008‑M2/129‑149 (MQ=255)
                   taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                                                                  >  1:1275485‑M2/129‑149 (MQ=255)
                    aaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCtt                                                                                                                                 <  2:453724‑M2/22‑1 (MQ=255)
                       ttttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                              <  1:102915‑M2/25‑1 (MQ=255)
                          tgccattttggtaaaacagtttcatcacaacaggagatagcaatcaccttaacgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtttattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                                                                           <  2:1473699‑M2/28‑1 (MQ=255)
                           gccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAt                                                                                                                          >  1:1246670‑M2/121‑149 (MQ=255)
                            ccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCTAtt                                                                                                                         >  2:1600912‑M2/120‑149 (MQ=255)
                             caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCTATTCTTTTTCGattt                                                                                                                        >  1:1314680‑M2/119‑148 (MQ=255)
                               atttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTACAAAGATAATCGATTCTTTTTCGATTGTc                                                                                                                      <  1:370283‑M2/33‑1 (MQ=255)
                                  tggtaatacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                   <  2:1626732‑M2/36‑1 (MQ=255)
                                  tggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                   <  1:265685‑M2/36‑1 (MQ=255)
                                    gtacaacagttgcatcacaacaggagatagcaatgacgttaccgagaggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtataatcgtgaaggtaAGTGCAAAGATACTCGATTCTTTTTCGATTGTCTGGCt                                                                                                                 <  2:1634466‑M2/38‑1 (MQ=255)
                                    gtaaaacagttgcatcacatcaggagatagcaatgacgttacctagttgacacaccaagagcagattgatcaaattatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                                                                                 <  1:1255641‑M2/38‑1 (MQ=255)
                                    gtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                                                                                 <  2:1656804‑M2/38‑1 (MQ=255)
                                    gtaaaaaagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                                                                                 <  2:1576685‑M2/38‑1 (MQ=255)
                                      aaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                               >  2:682632‑M2/110‑149 (MQ=255)
                                       aaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                                                                              <  2:1336191‑M2/41‑1 (MQ=255)
                                        aacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAGTGTCTGGCTGTAt                                                                                                             >  1:552634‑M2/108‑149 (MQ=255)
                                         acagttgcatcacaacaggatatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGTTAATCGATTCTTTTTCGATTGTCTGGCTGTATg                                                                                                            <  2:485514‑M2/43‑1 (MQ=255)
                                          cagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGc                                                                                                           <  1:239107‑M2/44‑1 (MQ=255)
                                               gcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCCGTATGCGTCaa                                                                                                      <  1:652673‑M2/49‑1 (MQ=255)
                                                catcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAAc                                                                                                     >  1:1064158‑M2/100‑149 (MQ=255)
                                                  tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                                                                   <  1:825597‑M2/52‑1 (MQ=255)
                                                  tcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                                                                   >  1:1152294‑M2/98‑149 (MQ=255)
                                                    acaacaggagatagcaaggacgttaccgagtggacacccgaagagcagattgatcaaaaaatttacagcactaggcccgtatattcgggaaggtaAGTGCAAAGATAATCGATTATTTTTCGATTGTCTGGCTGTATGCGTCAAAGTGa                                                                                                 >  2:860794‑M2/96‑149 (MQ=255)
                                                      aacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                                                                             >  2:1608661‑M2/94‑119 (MQ=255)
                                                      aacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                                                                             <  1:1608661‑M2/26‑1 (MQ=255)
                                                        caggagatagcaatgacgttaccgagtggacacccgaagagcagattgataaaaaaatttaccgcactaggcccggatattcgtgaaggtaAGTGCAATGATAATCTATTCTTTTTCGATTGTGTGGCTGTATGCGTCAACGTGAAGcc                                                                                             >  1:997640‑M2/92‑149 (MQ=255)
                                                                  caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAccggta                                                                                   >  1:425343‑M2/82‑147 (MQ=255)
                                                                  caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaACTGCAAAGATAATCGATTCTATTTCGATTGTCTGGCTGTATGCGTCAACGTGAATCCGGCACCGGaa                                                                                   >  1:375328‑M2/82‑149 (MQ=255)
                                                                    atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCTCTAGTCTGGCTTTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                                 >  2:490973‑M2/80‑149 (MQ=255)
                                                                    atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                                 <  2:967464‑M2/70‑1 (MQ=255)
                                                                      gacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGc                                                                               <  2:815889‑M2/72‑1 (MQ=255)
                                                                        cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                             <  1:632530‑M2/74‑1 (MQ=255)
                                                                        cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                             <  1:255713‑M2/74‑1 (MQ=255)
                                                                        cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcacgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                             <  1:382958‑M2/74‑1 (MQ=255)
                                                                        acttacctagtggacaccctaagagcagatttataaaaaaatgtaacgcactaggcactgatattcgtgaaggtaAGTGCAAAGATAAGCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                             <  1:1202078‑M2/74‑1 (MQ=255)
                                                                         gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTg                                                                            >  2:909800‑M2/75‑149 (MQ=255)
                                                                         gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAAAGTGAAACCGGCACCGGAAGTGCGTg                                                                            >  2:1244474‑M2/75‑149 (MQ=255)
                                                                         gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTATCGATTGTCTTGCTGTTTGCGTCAACGTGAAACCGGCACCGGAAGTgcggg                                                                            >  2:1717355‑M2/75‑147 (MQ=255)
                                                                               gagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt                                                                      <  1:909800‑M2/81‑1 (MQ=255)
                                                                                agtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtataatcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                                     <  2:226174‑M2/82‑1 (MQ=255)
                                                                                 gttgacacacgatgagcagattgttcaaaaaattttccgcaggtggcccgtatattcgggaaggtaAGTGCAACGATATTCGAGTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                                                                    <  1:1206678‑M2/83‑1 (MQ=255)
                                                                                  tggacacacgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTggg                                                                   >  1:425047‑M2/66‑149 (MQ=255)
                                                                                    gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGc                                                                 >  2:911521‑M2/64‑149 (MQ=255)
                                                                                       acccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCTGAAGTGCGTGAATTCTGGGGCtgg                                                              >  1:1031856‑M2/61‑149 (MQ=255)
                                                                                          cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg                                                           <  1:1443853‑M2/92‑1 (MQ=255)
                                                                                           gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtgga                                                          >  1:263097‑M2/57‑149 (MQ=255)
                                                                                            aagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat                                                         <  2:724766‑M2/94‑1 (MQ=255)
                                                                                                  agattgatcaaaaaattaaccgcactaggcccgtatattcgtgaaggaaAGGGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCTACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCt                                                   <  2:304940‑M2/100‑1 (MQ=255)
                                                                                                    attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTgg                                                 <  2:12596‑M2/102‑1 (MQ=255)
                                                                                                    attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATAGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCTTGAATTATGGGGCTGGTGGATGGAGCTgg                                                 >  2:1743703‑M2/48‑149 (MQ=255)
                                                                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                          >  1:416971‑M2/41‑149 (MQ=255)
                                                                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                          >  1:1315286‑M2/41‑149 (MQ=255)
                                                                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                          >  1:1838312‑M2/41‑149 (MQ=255)
                                                                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                          >  2:1531582‑M2/41‑149 (MQ=255)
                                                                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                          >  2:1328713‑M2/41‑149 (MQ=255)
                                                                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAAAGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCAGGTGGATGGAGCTCGAAGCGCa                                          >  1:1406441‑M2/41‑149 (MQ=255)
                                                                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTATTCGATTGTCTGGCTGTATGCGTCAACGTGCAACCGGCACCGGAAGTGCGTGAATTATGGGGCTGGTGGATGGAGCTGGAAGCGCa                                          >  2:279356‑M2/41‑149 (MQ=255)
                                                                                                            aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCCGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAg                                         >  1:974679‑M2/40‑149 (MQ=255)
                                                                                                              aaatttaccgcactaggcccgtatattcgtgaaggtaAGAGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCGGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGa                                       <  1:860794‑M2/112‑1 (MQ=255)
                                                                                                              aaagttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCTATTCTATTTCGAGTGTCTGGCGGTATGCGTCAACGTGAAACCGGCACCGGAAGGGCGTGAAGTCTGGGGCGGGTGGATGGAGCTGGAAGCGCAGGa                                       >  1:1625350‑M2/38‑149 (MQ=255)
                                                                                                                   taccgcactacgcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGGCGGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGGGCGTTAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATccc                                  <  1:585790‑M2/117‑1 (MQ=255)
                                                                                                                      cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAATCGTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTATGGGGCAGGTGGATGGAGCTGGAAACGCAGGAATACCGtt                               >  2:1232618‑M2/30‑149 (MQ=255)
                                                                                                                        cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtttt                             >  2:1406619‑M2/28‑149 (MQ=255)
                                                                                                                             cccccgtataatcgtgacggtaAGTGCAAAGATAATCGATGCCTTTTCGAATGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGGGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTa                        <  2:1086095‑M2/127‑1 (MQ=255)
                                                                                                                                ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAg                     >  2:1526503‑M2/20‑149 (MQ=255)
                                                                                                                                ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCAGTATGCGTCAACGTGAACCCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAg                     >  1:591995‑M2/20‑149 (MQ=255)
                                                                                                                                    atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc                 >  1:281797‑M2/16‑149 (MQ=255)
                                                                                                                                      attcgtgaaggtaAGTGCAAAGATTATCGATTCTTTTTCGATTGTCTGGCTGTATGCGGCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCa               <  1:1559843‑M2/136‑1 (MQ=255)
                                                                                                                                      attcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCa               >  1:1286154‑M2/14‑149 (MQ=255)
                                                                                                                                      attcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGGCAACGTTAAACAGTCACCGGAATTGAGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCa               <  1:1732893‑M2/136‑1 (MQ=255)
                                                                                                                                         cgtGAAGGTTAGTGCATAGATATTCGATTCTTTTTCGATTTTCTTGCTGTATGCGTCATCGTGAAACCGGCACCGGAATTGCGTGAATTCTGGGGCTGGTGGTTGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTTCAGTTACCAGtt            <  1:599637/147‑1 (MQ=255)
                                                                                                                                          tagaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATTCGTCAACGTGTTACCGGCACTGGAAGTGCGTGAATTCTGGTGCTGGTGGATGGAGCTGGAAGCGCTGGAATCCCGTTTTACCTACAGTTACCAGttt           <  1:219615‑M2/140‑1 (MQ=255)
                                                                                                                                          gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGACTCCCGATTTACCTACAGTTACCAGttt           >  2:1241286‑M2/10‑149 (MQ=255)
                                                                                                                                          gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt           >  2:351244‑M2/10‑149 (MQ=255)
                                                                                                                                           tgaaggtaAGTGCATAGATTATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg          <  1:1717355‑M2/141‑1 (MQ=255)
                                                                                                                                            gaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGTGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg         >  1:115437‑M2/8‑149 (MQ=255)
                                                                                                                                             aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt        >  1:24755‑M2/7‑149 (MQ=255)
                                                                                                                                                gtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTg     <  1:1406619‑M2/146‑1 (MQ=255)
                                                                                                                                                  aAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGtt   <  2:1166740‑M2/148‑1 (MQ=255)
                                                                                                                                                  aAGTGCAAACATAATCGATTCTTTTTCGCTTGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGtt   <  2:463892‑M2/148‑1 (MQ=255)
                                                                                                                                                   aGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGCCTGTTc  <  1:1526503/149‑1 (MQ=255)
                                                                                                                                                   aGTGAAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTc  <  2:24755/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTC  >  NC_000913/258537‑258832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: