| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,037,225 | 0 | A | C | 40.0% | 7.3 / 12.0 | 20 | intergenic (+8/‑35) | ileT/alaT | tRNA‑Ile/tRNA‑Ala |
| Reads supporting (aligned to +/- strand): ref base A (10/2); new base C (0/8); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAG > NC_000913/4037077‑4037241 | aaaGAAGGGTTCTTTGAAGTGCTCACACAGATTTTCTTATGAAAATGAGGAGTAAAACCTCTAGAGGCTTGTAGCTGAGGTGGTTAGAGCGCACCGCTGATAAGGGGGTGGTCGGTGGTTAAAGTCCACTTAGGCCTACCAAATTTGCa < 2:657890/149‑1 (MQ=255)aaaGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCa < 2:294727/149‑1 (MQ=34)aaaGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCa < 2:1841063/149‑1 (MQ=34) aagCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGGTCAAGTCCAATCAGGCCTACCAAATTTGCACGGc > 1:1266876/1‑149 (MQ=21) gTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGACGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTACACGTCAAAt > 2:750375/1‑149 (MQ=255) ttCTTTGAAGTGCTCACACATATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTATAGCTAACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGTCAAAtt > 2:1224534/1‑149 (MQ=11) ttCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAAtt > 2:324938/1‑149 (MQ=33) tCTTTGAAGTGCTCACACAGATTGTCTGATGAAACTGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAAttt < 1:129144/149‑1 (MQ=17) tCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCCCTCCGGCCTACAAAATTTGCACGGCAAAttt > 2:1444679/1‑149 (MQ=12) tCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCCGTGCAAAttt < 1:297564/149‑1 (MQ=12) tCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTCTACCTCACGTGGTTAGAGCGCACCCCAGATAAGGGTGAGGACGGAGGTTAAAGTCCACTAAGGCCTACAAAATATACACGGAAAAatt > 2:85756/1‑149 (MQ=11) cattGAAGTGCTCACACAGCTTGACGGATGAAAATGAGCAGTAAAACCGCAACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCCGTGCAAATTTg < 2:1139244/147‑1 (MQ=11) cTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCCGTGCAAATTTg < 1:510063/149‑1 (MQ=255) tttGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAACCCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCAGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCCGTGCAAATTTgg < 1:319831/149‑2 (MQ=11) tttGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGa < 2:256360/149‑1 (MQ=32) ttGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGaa > 1:472861/1‑149 (MQ=32) tGAAGTGCTCACACAGATTGTCTGATGAAAATGATCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCCGTGCAAATTTggta < 2:663755/149‑4 (MQ=11) tGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACACGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATCTGCCGTGCAAATTTggta < 2:29387/149‑4 (MQ=11) tGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTAAACAGGGTTGTAGCTCAGGGGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGGTCAAGGCCACTCAGGCCTACCAAATTTGCCGTGCAAATTTggta < 1:1590606/149‑4 (MQ=11) gAAGTGCTCACCCAGCTTGCCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCGGATACGGGTGAGGGCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCCGTGCAAATTTggtag < 1:1611717/149‑5 (MQ=11) gAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAaga > 2:576073/1‑149 (MQ=31) aaGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAtgag > 2:709165/1‑149 (MQ=11) aaGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAagag > 2:717152/1‑149 (MQ=11) | AAAGAAGCGTTCTTTGAAGTGCTCACACAGATTGTCTGATGAAAATGAGCAGTAAAACCTCTACAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTACCAAATTTGCACGGCAAATTTGAAGAG > NC_000913/4037077‑4037241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |