Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,088,193 C→A intergenic (+333/‑91) metK → / → galP S‑adenosylmethionine synthetase/D‑galactose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,088,1930CA97.3% 453.5 / 0.9 151intergenic (+333/‑91)metK/galPS‑adenosylmethionine synthetase/D‑galactose transporter
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (68/78);  total (70/81)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

Only 100 of 151 total aligned reads displayed.

TTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTTCGTCT  >  NC_000913/3088050‑3088338
                                                                                                                                               |                                                                                                                                                 
ttCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTa                                                                                                                                              <  1:986829/149‑1 (MQ=255)
  cATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCGGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTaca                                                                                                                                            >  1:1070019/1‑149 (MQ=255)
   atatTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTacac                                                                                                                                           >  1:1180669/1‑149 (MQ=255)
      ttattattCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGATATTACACTGa                                                                                                                                        >  2:464441/1‑149 (MQ=255)
       tattattCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAATCTCTTACACtaac                                                                                                                                       <  1:386513/149‑4 (MQ=255)
        tttattCGGTTTTCCAATTTGTTACATTTCTTTTCAGTATAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAATCTCTTACACtaaca                                                                                                                                      <  2:177280/148‑5 (MQ=255)
        attattCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAATCTCTTACACtaaca                                                                                                                                      <  1:1365503/149‑5 (MQ=255)
         ttattCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGAt                                                                                                                                       <  1:929367/147‑1 (MQ=255)
         ttattCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGAt                                                                                                                                       >  2:929367/1‑147 (MQ=255)
            ttCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAATGTCTTAATTGCAGATAACAGCGTTTAATCTATGATTATATAACTCAATTATTTTCATGCACATAAATAATAACTAAGATTAATGTTATTTTAAGTGTTTAGACTGATGTGAt                                                                                                                                  >  2:1673275/1‑149 (MQ=255)
             tctgTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATTATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGAtt                                                                                                                                 <  1:1104748/146‑1 (MQ=255)
             tCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGAtt                                                                                                                                 <  1:1583031/149‑1 (MQ=255)
                gTTTTCTCAGTTGTTACATTTCTTTTGAGTAAAGTCTTATTTGCAGATAACAGCGTTTAATCTATGATGATATATCTCAATTATTTTCATTCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTTCACTGATGTGATTTGc                                                                                                                              <  2:1468859/149‑1 (MQ=255)
                 ttttCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCt                                                                                                                             <  1:553338/149‑1 (MQ=255)
                  tttCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCtt                                                                                                                            <  2:1497984/149‑1 (MQ=255)
                  tttCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTATCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCtt                                                                                                                            <  2:1609045/149‑1 (MQ=255)
                   ttCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTc                                                                                                                           <  1:1581888/149‑1 (MQ=255)
                    tCAGAGTTGTTTCATTTCTTTTCAGTAAAGTCTTAATTGCTGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTca                                                                                                                          <  2:1212299/149‑1 (MQ=255)
                      acaGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTcaca                                                                                                                        >  1:1302522/1‑149 (MQ=255)
                      acaGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAAATAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTcaca                                                                                                                        >  2:1272331/1‑149 (MQ=255)
                         ttttttACATTTCTTTTAAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCAATTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCt                                                                                                                     <  2:209755/145‑1 (MQ=255)
                          ttgttACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCtt                                                                                                                    >  2:947452/1‑149 (MQ=255)
                            gttACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCtttt                                                                                                                  >  2:405769/1‑149 (MQ=255)
                             ttACATTTCTTTTCAGTAAAGTCTAAATTGCAGATAACAGCGTTTAATCTATCATGATATAACTCAATTATTTTCATGCACTGAAATCATAACTAAGATAAATGTTAGTGTAAGACATTACACTGATGTGATTTGCTTCACATCttttt                                                                                                                 <  2:1515876/149‑1 (MQ=255)
                             ttACATTTCTTTTCAGTAAAGTATTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCttttt                                                                                                                 <  2:1307987/149‑1 (MQ=255)
                                 aTTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTAcgt                                                                                                             >  2:1182268/1‑149 (MQ=255)
                                  tttCTTTTCAGTAAATTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTAcgtc                                                                                                            <  1:1012811/149‑1 (MQ=255)
                                  tttCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTActtc                                                                                                            >  1:1624846/1‑149 (MQ=255)
                                  tttCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTAcgtc                                                                                                            <  1:1322091/149‑1 (MQ=255)
                                  tttCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTAcgtc                                                                                                            >  2:860674/1‑149 (MQ=255)
                                  tttCTTATCACTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCAAGCACTTAAATCATAACTAAGAAAAATGTTAGTGAAAGACATTACACTGATGTGATTTGCTTCACATCTTTTTAcgtc                                                                                                            <  2:305723/149‑1 (MQ=255)
                                      ttttCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTAc                                                                                                        <  1:515222/149‑1 (MQ=255)
                                      ttttCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTAc                                                                                                        <  2:897096/149‑1 (MQ=255)
                                       tttCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACt                                                                                                       <  1:178130/149‑1 (MQ=255)
                                          cAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCAc                                                                                                    <  1:494967/149‑1 (MQ=255)
                                          aaGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCAc                                                                                                    <  2:765004/148‑1 (MQ=255)
                                           aGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCAGAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATATGATTTGCTTCACATCTTTTTACGTCGTACTCAcc                                                                                                   >  2:1400697/1‑149 (MQ=255)
                                                  tCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTa                                                                                            <  2:571068/149‑1 (MQ=255)
                                                    ttAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAt                                                                                          <  2:991213/149‑1 (MQ=255)
                                                     tAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAATATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAtt                                                                                         <  1:602597/149‑1 (MQ=255)
                                                     tAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAAtt                                                                                         <  2:736553/149‑1 (MQ=255)
                                                       aTTGTAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTca                                                                                       <  2:434417/149‑1 (MQ=255)
                                                        ttGAAGATAACAGAGTTTAATCTATTATGATATATCTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTcac                                                                                      <  1:1819560/149‑1 (MQ=255)
                                                              aTAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATaaa                                                                                >  2:1563671/1‑149 (MQ=255)
                                                              aTAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTAAGTCGTACTCACCTATCTTAATTCACAATaaa                                                                                >  1:458584/1‑149 (MQ=255)
                                                                   atcgattAATCTATGATTATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTTAATTGCTTCACATCTTTTTACGTCGTACTCACCAATCTTAATTCACAATAAAAAATa                                                                           <  1:1312103/144‑1 (MQ=255)
                                                                   aGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATa                                                                           >  1:784817/1‑149 (MQ=255)
                                                                   aGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAACATAAATGTTAGTGTAAGAGATTAACCTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTGAATTCACAATAAAAAATa                                                                           >  2:1270463/1‑149 (MQ=255)
                                                                   aGCGTTTAATCTATGATGATATAACTCAATTATTATCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGAACTCACATATCTTAATTCACAATAAAAAATa                                                                           >  2:1393454/1‑149 (MQ=255)
                                                                    tcGTTTAATCTATGTTGATATAACTCAATTATTTTCATGCACTTAATTCATATCTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACTTATCTTATTTCACAATAAAAAATaa                                                                          <  2:878333/148‑1 (MQ=255)
                                                                    tcGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATaa                                                                          <  2:1082248/148‑1 (MQ=255)
                                                                    gCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATaa                                                                          <  1:775618/149‑1 (MQ=255)
                                                                    gCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATaa                                                                          <  1:12108/149‑1 (MQ=255)
                                                                     cGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAAc                                                                         >  2:419957/1‑149 (MQ=255)
                                                                      gTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAAcc                                                                        >  1:664465/1‑149 (MQ=255)
                                                                           aTCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATAtt                                                                   >  1:315068/1‑149 (MQ=255)
                                                                            tCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTg                                                                  >  2:324422/1‑149 (MQ=255)
                                                                              tATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGa                                                                >  2:1322068/1‑149 (MQ=255)
                                                                               atgatgATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGCTTACACTGATGTGAGTTGCTTCACATCTTTTTACGTCGAACTCACCTATCTTAATACACAATAAAAAATAACCATATTggcg                                                               >  2:1209796/1‑147 (MQ=255)
                                                                                  atgatATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAggcc                                                            >  1:670767/1‑147 (MQ=255)
                                                                                     atatAACTCAATTATTTTCATGCACTTAAATCATAGCTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGcatc                                                         <  2:126777/149‑1 (MQ=255)
                                                                                       ataACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTCTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGcatcat                                                       >  2:535613/1‑149 (MQ=255)
                                                                                          aCTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGcc                                                    >  2:22129/1‑149 (MQ=255)
                                                                                           cTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGTGCATCATGCCt                                                   >  1:1497599/1‑149 (MQ=255)
                                                                                            tCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTg                                                  >  1:601693/1‑149 (MQ=255)
                                                                                            tCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTg                                                  >  2:421477/1‑149 (MQ=255)
                                                                                            tCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTACCATATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTg                                                  >  1:1694643/1‑149 (MQ=255)
                                                                                              aaTTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGAc                                                <  1:1843240/149‑1 (MQ=255)
                                                                                              aaTTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGAc                                                >  1:1502239/1‑149 (MQ=255)
                                                                                              aaTTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGAc                                                <  1:1559611/149‑1 (MQ=255)
                                                                                                   ttttCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTaa                                           <  1:1563671/149‑1 (MQ=255)
                                                                                                     ttCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTaaaa                                         >  1:583310/1‑149 (MQ=255)
                                                                                                         tGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGTAGGGCATCATGCCTGACGCTAAAAAACa                                     <  2:1585014/149‑1 (MQ=255)
                                                                                                         tGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACa                                     <  2:1338476/149‑1 (MQ=255)
                                                                                                         tGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACa                                     <  2:784817/149‑1 (MQ=255)
                                                                                                          tcACTTAAATCATAACTAAGATATATGTTAGTGTAATAGATTACACTGATTTGATTTGCTTCACATCTTTTTACGTCGTTCTCAACTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAg                                    <  1:1018202/148‑1 (MQ=255)
                                                                                                            aCTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTAcgtcg                                                                                                           >  1:607133/1‑76 (MQ=255)
                                                                                                            aCTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTAcgtcg                                                                                                           <  2:607133/76‑1 (MQ=255)
                                                                                                            aCTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACTTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATAGGAGGGCATCATGCCTAACACTAAAAAACaatg                                  >  2:1260390/1‑146 (MQ=255)
                                                                                                            aCTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAggg                                  >  1:245346/1‑149 (MQ=255)
                                                                                                            aCTTAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAggg                                  <  1:947452/149‑1 (MQ=255)
                                                                                                              ttAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACTCTAAAAAACAgggtc                                >  2:1080659/1‑147 (MQ=255)
                                                                                                              ttAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGc                                >  1:1180466/1‑149 (MQ=255)
                                                                                                              ttAAATCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGc                                <  2:664465/149‑1 (MQ=255)
                                                                                                                 aaTCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGt                             <  1:608497/149‑1 (MQ=255)
                                                                                                                  aTCATAACTAAGTTAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTc                            >  2:488252/1‑149 (MQ=255)
                                                                                                                   tCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACGTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTCACGCTAAAAAACAgggcagctca                           >  2:1220156/1‑142 (MQ=255)
                                                                                                                   tCATAACTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCa                           >  2:1156737/1‑149 (MQ=255)
                                                                                                                        aCTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACaa                      >  2:1613134/1‑149 (MQ=255)
                                                                                                                         cttaGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCAGATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAg                     <  2:983737/146‑1 (MQ=255)
                                                                                                                         cTAAGATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAg                     >  2:639609/1‑149 (MQ=255)
                                                                                                                             gATAAATGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCaa                 >  2:975355/1‑149 (MQ=255)
                                                                                                                                aaaTGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGa              <  1:1269247/149‑1 (MQ=255)
                                                                                                                                 aaTGTTAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGAc             >  1:988525/1‑149 (MQ=255)
                                                                                                                                     ttAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCTCCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACACGGCAATGACGttt         <  2:410333/149‑1 (MQ=255)
                                                                                                                                     ttAGTGTAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAACGAGGGGCGGTCACACCAGGCAATGACGttt         >  2:1085444/1‑149 (MQ=255)
                                                                                                                                        gtgtAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTTc      <  1:858252/149‑1 (MQ=255)
                                                                                                                                        gtgtAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTTc      <  1:754183/149‑1 (MQ=255)
                                                                                                                                          gtAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCAGCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTTCGt    <  2:1070019/149‑1 (MQ=255)
                                                                                                                                           tAAGAGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTTCGTc   >  1:294432/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
TTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCACAATAAAAAATAACCATATTGGAGGGCATCATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTTCGTCT  >  NC_000913/3088050‑3088338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: