Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,157,869 Δ18 bp coding (1‑18/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1157869 1157886 18 104 [0] [0] 103 ptsG fused glucose‑specific PTS enzymes: IIB component/IIC component

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 11578680 (0.000)103 (1.040) 73/290 0.2 100% intergenic (+294/‑1) ycfH/ptsG putative DNase/fused glucose‑specific PTS enzymes: IIB component/IIC component
?NC_000913 1157887 = 0 (0.000)coding (19/1434 nt) ptsG fused glucose‑specific PTS enzymes: IIB component/IIC component

Only 100 of 103 total aligned reads displayed.

GGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCG  >  NC_000913/1157740‑1158031
                                                                                                                                                   |                                                                                                                                                
atagccgtcaaacaaattggcactgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGc                                                                                                                                                 <  2:1624310‑M2/2‑1 (MQ=255)
   gccgtcaaacaaattggcactgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCtac                                                                                                                                              >  2:528325‑M2/145‑148 (MQ=255)
   gccgtcaaacaaattggcactgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCtac                                                                                                                                              >  2:14002‑M2/145‑148 (MQ=255)
   gccgtcaaacaaattggcactgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTaa                                                                                                                                              <  1:307720‑M2/5‑1 (MQ=255)
             aaattggcactgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAg                                                                                                                                    <  2:59280‑M2/15‑1 (MQ=255)
             aaattggcactgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAg                                                                                                                                    <  2:1432941‑M2/15‑1 (MQ=255)
             aaattggcactgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgggagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAg                                                                                                                                    >  2:162017‑M2/135‑149 (MQ=255)
             aaattggcactgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggcgctccccccttgccacgcgggagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAg                                                                                                                                    <  2:1266688‑M2/15‑1 (MQ=255)
              aattggcactgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAgg                                                                                                                                   <  2:377400‑M2/16‑1 (MQ=255)
               attggcactgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGt                                                                                                                                  <  1:252580‑M2/17‑1 (MQ=255)
                ttggcactgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTc                                                                                                                                 <  1:853376‑M2/18‑1 (MQ=255)
                       tgaattattttactctgtgtaataaataaaggtcgcttagatgccctttacacggcgaggctctcccccattgccacgcgtgagaacgtaaaaaaagctcccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAAt                                                                                                                          <  1:117006‑M2/25‑1 (MQ=255)
                       tgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAAt                                                                                                                          >  1:389805‑M2/125‑149 (MQ=255)
                       tgaattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctcccccattgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAAt                                                                                                                          >  1:888860‑M2/125‑149 (MQ=255)
                       tgaattattttactctgtgtaataaataaagggcgcttagatgacctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccataatcaggagcagtctcaattGCTAACAGGCAAAAGGTGGgtaact                                                                                                                          >  2:1032213‑M2/125‑147 (MQ=255)
                          attattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGc                                                                                                                       <  1:832634‑M2/28‑1 (MQ=255)
                          aattattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctcccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGc                                                                                                                       <  2:636929‑M2/28‑1 (MQ=255)
                           ttattttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCt                                                                                                                      <  2:233018‑M2/29‑1 (MQ=255)
                              ttttactctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAGCCTGCAAAAGGTCGGTAAATCGCGgag                                                                                                                   >  2:721640‑M2/118‑148 (MQ=255)
                                    tctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaaggtaaaaaaagcacccaaactcaggagcactcacaattGCTAACCTCCAAAAGGTCGGTAAATCGCTGATGCTGcc                                                                                                             >  2:432393‑M2/112‑149 (MQ=255)
                                    tctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcgcccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGcc                                                                                                             >  1:912178‑M2/112‑149 (MQ=255)
                                    tctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGcc                                                                                                             >  1:1547228‑M2/112‑149 (MQ=255)
                                     ctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgcctcgggtgagaacgtaaaacaagcagccatactctggagctctctcaattGCTAACCAGCAAATGGTCGGTAAATCGCTGCTGCTGACg                                                                                                            >  2:1349640‑M2/111‑149 (MQ=255)
                                     ctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCg                                                                                                            >  2:1556312‑M2/111‑149 (MQ=255)
                                     ctgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCg                                                                                                            <  1:696252‑M2/39‑1 (MQ=255)
                                      tgtgtaataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCAGATGCTGCCgg                                                                                                           >  1:1194465‑M2/110‑149 (MQ=255)
                                          taataaataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccccgcgtgagaacgtaaaaaaagcccccatactcaggagcactctcaattGCTAACATGCAAAAGGTCGGTAATGCGCTGATCCTGCCGGTATc                                                                                                       >  2:1057974‑M2/106‑149 (MQ=255)
                                           aataaataaacggcgcttagatgcgctgtacccggcgaggctctccccccttgccacgcgtgagaacgtaaaaaacgcacccatactcagtagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATcc                                                                                                      <  2:1205474‑M2/45‑1 (MQ=255)
                                               aataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTAc                                                                                                  >  1:206168‑M2/101‑149 (MQ=255)
                                               aataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTAc                                                                                                  >  1:56192‑M2/101‑149 (MQ=255)
                                                ataaagggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACt                                                                                                 >  1:1165242‑M2/100‑149 (MQ=255)
                                                    agggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTg                                                                                                <  1:994528‑M2/51‑1 (MQ=255)
                                                    agggcgcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTg                                                                                                >  2:994528‑M2/96‑146 (MQ=255)
                                                         gcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGc                                                                                        >  2:206649‑M2/91‑149 (MQ=255)
                                                         gcttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGc                                                                                        >  1:357327‑M2/91‑149 (MQ=255)
                                                          cttagatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCa                                                                                       <  1:813018‑M2/60‑1 (MQ=255)
                                                            tagatgccctgtacacggctaggctcaccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAgg                                                                                     <  1:1096777‑M2/62‑1 (MQ=255)
                                                             agatgccctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCTGTAAATCGCTGATGCTGCCGGAATCCGTACTGACTATCGCAGGt                                                                                    >  2:1488554‑M2/87‑149 (MQ=255)
                                                                   cctgtacacggcgaggctctccccccttgccacgcgtgagaacgttaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTctg                                                                              >  1:745942‑M2/81‑149 (MQ=255)
                                                                    ctgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTctgc                                                                             >  2:67866‑M2/80‑149 (MQ=255)
                                                                    ctgtacacggcgaggctctccccacttgccacgcgtgagaccgtaaaaaaagcacccaaactcaggagcactcacaattGCTAACCTGCAAAACGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGTTATtctgt                                                                             >  2:1247957‑M2/80‑148 (MQ=255)
                                                                    atgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTctgc                                                                             >  2:1241905‑M2/80‑149 (MQ=255)
                                                                     tgtacacggcgtggcgctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTctgct                                                                            <  1:744301‑M2/71‑1 (MQ=255)
                                                                     tgtacacggcgaggctctccccccttgccacgcgtgataacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTctgct                                                                            <  1:721640‑M2/71‑1 (MQ=255)
                                                                     tgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcagtctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGAAGGTATTctgct                                                                            >  2:1228519‑M2/79‑149 (MQ=255)
                                                                     tgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTctgct                                                                            <  2:389805‑M2/71‑1 (MQ=255)
                                                                     tgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTctgct                                                                            <  2:1622634‑M2/71‑1 (MQ=255)
                                                                     tgtacacggcgaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacacatactcaggagcactgtctattGCTATCCTGCAAAGGGTCGGTTAATCGCGGATGCTGCCCGTATCCGATCTGCCTATCGCaggtattaggct                                                                            >  2:915885‑M2/79‑144 (MQ=255)
                                                                     tgtacacggcgaggctctccccacttgccacgcgtgagaacttaaaaaaagcacccataatcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCTTACTGCCTATCGCAGGTATTctgct                                                                            <  2:888860‑M2/71‑1 (MQ=255)
                                                                          acggcgaggctctccccccttgccacgcgggagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCg                                                                       <  1:162017‑M2/76‑1 (MQ=255)
                                                                          acggagaggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCg                                                                       >  2:1214670‑M2/74‑149 (MQ=255)
                                                                                 ggctctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTc                                                                <  2:357327‑M2/83‑1 (MQ=255)
                                                                                  gatctccccccttgccacgcgtgagaacgtaaaaaacgcacgcattctcaggagcactctcaattGCTAACCTGCAAATGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTcc                                                               <  1:432393‑M2/84‑1 (MQ=255)
                                                                                    tctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCcgc                                                             <  2:1165242‑M2/86‑1 (MQ=255)
                                                                                     ctccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcatttGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTAGTCTGCTGGGCGTCGGTTCcgcg                                                            >  1:264392‑M2/63‑149 (MQ=255)
                                                                                       ccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTACCCTGCAAAAGGTCGGTAACTCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGaa                                                          <  1:1153580‑M2/89‑1 (MQ=255)
                                                                                       ccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGaa                                                          <  1:1105366‑M2/89‑1 (MQ=255)
                                                                                       ccccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGGCGGTAAATCGCTGTTGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGaa                                                          <  2:76480‑M2/89‑1 (MQ=255)
                                                                                        cccccttgccacgcgtgagaacgtaaaaaaggcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAAt                                                         <  2:235306‑M2/90‑1 (MQ=255)
                                                                                        cccccttgccacgcgtgagaacgtaaaaaaggcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAAt                                                         >  1:235306‑M2/60‑149 (MQ=255)
                                                                                         ccccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAAtt                                                        >  2:778656‑M2/59‑149 (MQ=255)
                                                                                          cccttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAAttt                                                       >  2:341175‑M2/58‑149 (MQ=255)
                                                                                            cttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCAGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCa                                                     <  2:1194465‑M2/94‑1 (MQ=255)
                                                                                             ttgccacgcgtgagatcgtaaaataagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAg                                                    <  2:912178‑M2/95‑1 (MQ=255)
                                                                                             ttgccacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAg                                                    <  2:1547228‑M2/95‑1 (MQ=255)
                                                                                                 cacgcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAgctgg                                                >  1:1170633‑M2/51‑149 (MQ=255)
                                                                                                    gcgtgagaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAgctggctg                                             >  1:1054006‑M2/48‑149 (MQ=255)
                                                                                                          gaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCa                                                     <  2:523061‑M2/94‑1 (MQ=255)
                                                                                                          gaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCa                                                     >  1:523061‑M2/42‑135 (MQ=255)
                                                                                                          gaacgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTCCCGGTATCCGTACTGCCTATCGCAGGTGTTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCccgcc                                       >  1:1205171‑M2/42‑149 (MQ=255)
                                                                                                             cgtaaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGtt                                    <  1:341175‑M2/111‑1 (MQ=255)
                                                                                                               taaaaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGt                                  >  1:731259‑M2/37‑149 (MQ=255)
                                                                                                                  aaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCTGTATCCGTACTGCCTATCGCAGGTATACTGCTGGGCGTCCGTTCCGCGAATTTCAGCTCGCTGCCCGCCGTTgtaac                               >  1:694905‑M2/34‑147 (MQ=255)
                                                                                                                  aaaaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATc                               <  2:206168‑M2/116‑1 (MQ=255)
                                                                                                                    aaagcacccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGc                             <  1:1488554‑M2/118‑1 (MQ=255)
                                                                                                                          cccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATgttt                       >  2:783268‑M2/26‑148 (MQ=255)
                                                                                                                          cccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTa                       <  1:1556312‑M2/124‑1 (MQ=255)
                                                                                                                           ccatactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTAt                      >  1:155396‑M2/25‑149 (MQ=255)
                                                                                                                            catactcaggagcactctcaattGCTAACCTGCAAAAGTACGGTAAATCGCTGATGCTCCCGGTATCCGTACTGCCTATCGCAGGAATTCTGCTGGGCGTCGGTTCCGCGAATTTCATCTGGCTGCCCGCCGTTGTATCGCATTTTATg                     >  1:366256‑M2/24‑149 (MQ=255)
                                                                                                                             atactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCTCAGGTATTCTGCTGGGCGTCGGTTACGCGAATTTCAGCTGGCTGCCCTCCGTTGTATCGCAAGTTATgg                    >  2:774959‑M2/23‑149 (MQ=255)
                                                                                                                             atactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATgg                    >  1:909682‑M2/23‑149 (MQ=255)
                                                                                                                             atactcaggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGATGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATgg                    <  1:1057974‑M2/127‑1 (MQ=255)
                                                                                                                              tactcaggagccctctcaattGCTAACCTGCTAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGc                   <  1:1032213‑M2/128‑1 (MQ=255)
                                                                                                                                  caggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTGCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGaa               <  1:778656‑M2/132‑1 (MQ=255)
                                                                                                                                   aggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAg              >  1:430223‑M2/17‑149 (MQ=255)
                                                                                                                                    ggagcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCTCATGTTATGGCAGAaga             >  2:391671‑M2/16‑148 (MQ=255)
                                                                                                                                       gcactctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTGTTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAgg          <  2:1205171‑M2/137‑1 (MQ=255)
                                                                                                                                         actctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCg        <  1:206649‑M2/139‑1 (MQ=255)
                                                                                                                                         actctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGGTATGGCAGAAGCAGGCg        >  2:1372871‑M2/11‑149 (MQ=255)
                                                                                                                                          ctctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGAATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCgg       <  1:915885‑M2/140‑1 (MQ=255)
                                                                                                                                           tctcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGt      >  2:1241097‑M2/9‑149 (MQ=255)
                                                                                                                                             tcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTc    >  2:704233‑M2/7‑149 (MQ=255)
                                                                                                                                             tcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTc    >  2:492640‑M2/7‑149 (MQ=255)
                                                                                                                                             tcaattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTAACCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGATCCGCGAATTTCAGCTGGCTGCCAGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTc    >  1:1192665‑M2/7‑149 (MQ=255)
                                                                                                                                             tcaattGCTAACCTGCAAAAGGTCGCTAAAACGCTCATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGTTTCCGAGAATTTCAGCTGGCTGCCGGCCGTTGTATCGCATGTTATTGCATAAGCTGGCGGTTc    >  1:1262966‑M2/7‑149 (MQ=255)
                                                                                                                                              caattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCa                  >  2:649853‑M2/6‑134 (MQ=255)
                                                                                                                                              caattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCa                  <  1:649853‑M2/129‑1 (MQ=255)
                                                                                                                                               aattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCg  >  1:349521‑M2/5‑149 (MQ=255)
                                                                                                                                               aattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCg  >  2:244971‑M2/5‑149 (MQ=255)
                                                                                                                                               aattGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCAGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCg  >  1:635903‑M2/5‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCG  >  NC_000913/1157740‑1158031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: