Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,6620CG20.0% 74.3 / 11.4 41Q582E (CAA→GAA) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base C (11/21);  new base G (4/4);  total (16/25)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGT  >  NC_000913/1708523‑1708765
                                                                                                                                           |                                                                                                       
cAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCg                                                                                                >  1:1344478/1‑149 (MQ=255)
 agagCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGCAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGa                                                                                               <  2:1504414/149‑1 (MQ=255)
 agagCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGa                                                                                               >  2:1000743/1‑149 (MQ=255)
      gAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACTACAGGTCGATCcgt                                                                                          <  1:1186458/149‑2 (MQ=255)
           aGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGTAACCAGAAAAACACTACGATCCGCTCaaa                                                                                     >  1:1464894/1‑149 (MQ=25)
           aGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCaaa                                                                                     <  2:1264985/149‑1 (MQ=255)
            ggCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAg                                                                                    <  1:590887/149‑1 (MQ=255)
                gAACTGCAGCTAACTAACGACCCAGGAACCGTTGCTGTTCCACGTAAAACTGCCTTTGAAGCAGCTATCGCCCGCGCCAAACCGCGCTAGCTGGAACAGCAACAGGCTAATGCGGAACCAGATCATCAGGTCGATCCGCGCATAgccgc                                                                                <  2:389436/149‑1 (MQ=21)
                 aaCTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATACACGTAAAACTGCCGGTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAgccgcc                                                                               <  1:1219493/149‑1 (MQ=255)
                   gagcagcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGt                                                                             <  2:1039837/147‑1 (MQ=255)
                       agcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGaa                                                                         <  2:505654/149‑1 (MQ=255)
                       agcaAACTAACGACGCAGCAACCGGTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGaa                                                                         <  2:1249150/149‑1 (MQ=255)
                            aCTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGc                                                                    <  2:606074/149‑1 (MQ=255)
                                   acgCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATCGCGAACCAGAACACCAGGTAGATCCGCGCAAAGCCGCAGTCCAAGACCCGATTGcc                                                             >  1:1560159/1‑149 (MQ=18)
                                        gcaACCGTTGCTGATCCACGTAAATCTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACTGGCTAGTGAGGAGCCAGAACAACAGGTCGATCCGAGCAAAGCCGCGGGGGAAGCCGCTATTGCCCGTGc                                                        >  2:1247373/1‑149 (MQ=18)
                                        gcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGACCCAGAACAACACGTCGATCCGAGCAAAGCCGCCGTCGAAGCCGCTATTTACCGTGc                                                        >  1:431009/1‑149 (MQ=18)
                                        gcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGACCGTGc                                                        <  2:1344478/149‑1 (MQ=255)
                                         caACCGTTGCTGATCCACGTAAACCTGCCGTCGACGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGcc                                                       <  2:59124/149‑1 (MQ=255)
                                              gTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCACGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGATAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgc                                                  <  1:413641/149‑1 (MQ=255)
                                               ttGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGAGCCAAAGCACGCAAGCTGAAACACCACCAGTATAATGAGGAACAAGAAGAACCGGTCGATCCGCGCAAGGCCGACGACGAAGAAACTATTGCCCGTGCCAAAgcg                                                 >  2:257328/1‑149 (MQ=255)
                                                tGCGGAGCCACGTAAAACTGCCGATTAAGCAGCTATCGCCCGCGCCAAAGCGCGGAAGCTGGAACAGCTACAGGGTAATGCGGAATCAGAACAAGAGGTAGATCCGAGCAAATCCGCCGTCGAAGCCGATATTGCCCGTGCCAAAgcgc                                                <  2:1564452/149‑1 (MQ=255)
                                                ggccgATCCCCGCAAACCCGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcac                                                <  1:257328/145‑3 (MQ=1)
                                                       ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCTCGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATTAGGAACCAGAACAACATGTCGATCCTCGCAAAGCCGCCGTCGAAGCAGCTATTGCCAGTGCCAAAGCGCGCAAGCt                                         <  2:18326/149‑1 (MQ=17)
                                                       ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAACCGCGCAAGCTGGTACAGCAACAGGCTTATGCGGAACCAGAACAACAGGTCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCt                                         <  2:24390/149‑1 (MQ=255)
                                                           gTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGaa                                     <  1:855744/149‑1 (MQ=255)
                                                           gTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGATCGCGCCCCGGCACGCCGCCGGCGACGACGCGATTGCCCATGCCCAAGCGCGCAAGcagaaa                                     >  1:1526050/1‑144 (MQ=17)
                                                                  tGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccgc                                                                                <  1:416539/99‑1 (MQ=255)
                                                                  tGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccgc                                                                                >  2:416539/1‑99 (MQ=255)
                                                                              agcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATcc                                                                                            <  1:88480/75‑1 (MQ=25)
                                                                              agcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATcc                                                                                            >  2:88480/1‑75 (MQ=25)
                                                                                  aTCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAt                                                                                              <  1:767148/69‑1 (MQ=25)
                                                                                  aTCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAt                                                                                              >  2:767148/1‑69 (MQ=25)
                                                                                    ctcacgcgCCAAAGCTCGCAATCTGGAACATCAACAGGCAAATTCGGAACCAGAACAAGAGGTCGATCCGCGCAAACCAGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCag            <  1:744255/145‑1 (MQ=14)
                                                                                           gcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGc                                                                    >  2:769159/1‑86 (MQ=18)
                                                                                              aaaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGt  <  1:1000743/149‑1 (MQ=255)
                                                                                                 gcgcgcAAGCTGGAACAGCAACAGGCTAATGCGGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGc                                                        <  1:22827/92‑1 (MQ=16)
                                                                                                 gcgcgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGc                                                        >  2:22827/1‑92 (MQ=18)
                                                                                                    cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGc                                                        >  2:307768/1‑89 (MQ=33)
                                                                                                                   cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCg                                                           <  1:783013/71‑1 (MQ=34)
                                                                                                                   cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCg                                                           >  2:783013/1‑71 (MQ=34)
                                                                                                                    aaCAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTg                                                         >  2:862713/1‑72 (MQ=34)
                                                                                                                                           |                                                                                                       
CAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGT  >  NC_000913/1708523‑1708765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑