Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258676 258683 8–776 54 [0] [0] 55 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)49 (0.990) 43/266 0.2 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

CTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTC  >  NC_000913/258537‑258832
                                                                                                                                                   |                                                                                                                                                     
gcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                                                                      <  1:164215‑M2/2‑1 (MQ=255)
       ttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAg                                                                                                                                               >  1:308362‑M2/141‑149 (MQ=255)
         aaaaatttgctaaattttgccaatttggtacaacagttgcatcacagcaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGAGAAAAGAt                                                                                                                                             >  1:182267‑M2/139‑149 (MQ=255)
           aaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATaa                                                                                                                                           <  1:668560‑M2/13‑1 (MQ=255)
                   taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgaatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                                                                   >  2:103536‑M2/129‑149 (MQ=255)
                   taaattttgccaatttggtaaaacagttgcagcacaacaggagatagcaatgacgttaccgagtggacacacgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCt                                                                                                                                   >  2:289999‑M2/129‑149 (MQ=255)
                           ggcaatttggaaaaacagttgcatcacaaaaggagttagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAt                                                                                                                           <  2:182267‑M2/29‑1 (MQ=255)
                             caattgggtaaaacagtttcatcacaacaggagagagcaatgacgttaccgagtggacacccgaagagcagattgatcaaataatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTg                                                                                                                         <  2:777907‑M2/31‑1 (MQ=255)
                                    gtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTTTCTGGCt                                                                                                                  >  1:785036‑M2/112‑149 (MQ=255)
                                    ggaaaaccgttgcaacacaacaggagaaagcaatgacgtgaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                                                                                  <  2:13842‑M2/38‑1 (MQ=255)
                                     taaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTg                                                                                                                 >  2:718755‑M2/111‑149 (MQ=255)
                                          cagttgcatcacaacaggagatagcaatgacgttaacgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGc                                                                                                            <  2:135324‑M2/44‑1 (MQ=255)
                                                catcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGGGTCCAc                                                                                                      >  1:33125‑M2/100‑149 (MQ=255)
                                                    ccgacaggggaaagcaatggcgtgagggagtgctcacccgaagagcagattgatcaaaaaatttaccgcacttggcccgtatattcgtgaaggtaAGGGCAAAGATATTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGa                                                                                                  <  1:211201‑M2/54‑1 (MQ=255)
                                                      aacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgcatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTCTGcgtcaacctacaa                                                                                                >  2:826737‑M2/94‑143 (MQ=255)
                                                           gagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTGAACGTGAAACCGGc                                                                                           >  1:755483‑M2/89‑149 (MQ=255)
                                                           gagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCCGc                                                                                           >  1:889371‑M2/89‑149 (MQ=255)
                                                             gatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAc                                                                                         <  1:289999‑M2/63‑1 (MQ=255)
                                                                ggctgtgaggttaccaggtggtcatccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAGCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCgg                                                                                      <  2:95638‑M2/66‑1 (MQ=255)
                                                                agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCgg                                                                                      >  2:799109‑M2/84‑149 (MQ=255)
                                                                  caatgacgttaccgggtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAGCGTGAAACCGGCACCGGaa                                                                                    <  2:130094‑M2/68‑1 (MQ=255)
                                                                  caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                                                                    <  2:324818‑M2/52‑1 (MQ=255)
                                                                  caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                                                                    >  1:324818‑M2/82‑133 (MQ=255)
                                                                  caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGaa                                                                                    >  2:824704‑M2/82‑149 (MQ=255)
                                                                   aatgacgttaccgagtggacacccgaagagcagattgatcaaaacatttaccgcactaggcccgtatattcccgaagggcAGTGCAAAGATAATCGATTCTTTTTCGCTTGTCTGCCGCTCTGCGTCAACGTCCAaccggcccccgccg                                                                                   >  2:462741‑M2/81‑144 (MQ=255)
                                                                    atgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTCTGCGTCAACGTGAAACCGGCACCGGACGt                                                                                  >  2:35370‑M2/80‑149 (MQ=255)
                                                                        cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                              <  1:914060‑M2/74‑1 (MQ=255)
                                                                        cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                              <  2:770161‑M2/74‑1 (MQ=255)
                                                                        cgttaccgagtggacacccgaagagcaaattgatcaataactttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                              <  1:403707‑M2/74‑1 (MQ=255)
                                                                           taccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCCCCGGAAGGTCGTGaa                                                                           >  2:707107‑M2/73‑149 (MQ=255)
                                                                           taccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGaa                                                                           <  1:718755‑M2/77‑1 (MQ=255)
                                                                                agtggacaaccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCTTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                                      <  1:35370‑M2/82‑1 (MQ=255)
                                                                                  tggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTggg                                                                    <  1:778991‑M2/84‑1 (MQ=255)
                                                                                          cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTATGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTTGGTGAATTCTGGGGCtgatgg                                                            >  1:400936‑M2/58‑149 (MQ=255)
                                                                                           gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCg                                                                                                                                        <  1:3648‑M2/16‑1 (MQ=255)
                                                                                           gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCg                                                                                                                                        >  2:3648‑M2/57‑72 (MQ=255)
                                                                                                    attgagcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTgg                                                 <  2:308362‑M2/102‑1 (MQ=255)
                                                                                                      tgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                               <  1:535264‑M2/104‑1 (MQ=255)
                                                                                                       gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCCTGAATTCTGGGGCTGGTGGATGGAGATGGAAg                                              >  1:649305‑M2/45‑149 (MQ=255)
                                                                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCt                                                    <  1:243008‑M2/100‑1 (MQ=255)
                                                                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCt                                                    >  2:243008‑M2/41‑140 (MQ=255)
                                                                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                          <  1:799109‑M2/109‑1 (MQ=255)
                                                                                                               aatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                               >  2:470082‑M2/37‑140 (MQ=255)
                                                                                                               aatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGaa                                               <  1:470082‑M2/104‑1 (MQ=255)
                                                                                                                 tttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATc                                    <  2:274788‑M2/115‑1 (MQ=255)
                                                                                                                 tttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATc                                    >  1:911068‑M2/35‑149 (MQ=255)
                                                                                                                    accgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCAGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCg                                 >  2:13981‑M2/32‑149 (MQ=255)
                                                                                                                          ctaggcccgtatattcgtgaaggtaAGTGCAAAGGTAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                           <  1:362924‑M2/124‑1 (MQ=255)
                                                                                                                                ccgtatattcgtggaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGAGAAACCGGCACCGGAAGTGCGTGAATTCTTAGGCTGGTGGATGGAGCTGAGAAGGCAGGAATCCCGATTTACGTACAg                     >  2:857200‑M2/20‑149 (MQ=255)
                                                                                                                                            gaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgga                                                       <  1:912403‑M2/97‑1 (MQ=255)
                                                                                                                                            gaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgga                                                       >  2:912403‑M2/8‑104 (MQ=255)
                                                                                                                                                gtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGCAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGCATCCCGTTTTACCTACAGTTACCAGGTTGgtcgg     >  1:180945‑M2/4‑147 (MQ=255)
                                                                                                                                                gtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTg     >  1:657842‑M2/4‑149 (MQ=255)
                                                                                                                                                gtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGGCAACGTGAAACCGGAACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTg     >  1:806547‑M2/4‑149 (MQ=255)
                                                                                                                                                   aGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTc  >  2:650619/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTC  >  NC_000913/258537‑258832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.