Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT20.0% 124.2 / 25.9 75intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (29/31);  new base T (11/4);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCC  >  NC_000913/4295912‑4296207
                                                                                                                                                     |                                                                                                                                                   
gTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                                                                     <  2:118961/149‑1 (MQ=32)
gTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                                                                     <  1:233233/149‑1 (MQ=32)
 tCGGATGAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCCTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg                                                                                                                                                    <  1:428451/149‑1 (MQ=17)
 tCGGAAAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACg                                                                                                                                                    <  2:523239/149‑1 (MQ=17)
       tgggtgTTTATGCCGCCGCTGACATCATCGCATGATGCGAAGCTTAACGGGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt                                                                                                                                              <  2:77734/144‑1 (MQ=11)
          gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGTACTACGCCATACAGCGCAATAGCCTGATTTAGCGTGATTTTCTAGGTCGGATCAGGCGTTTACGCCGCATCCCACATCAATGCCTgttacg                                                                                                                                           >  2:337290/1‑144 (MQ=11)
          gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGACTTATAAGGCCTACGCCAGACAGCGCAATAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATAAACGCCTGATGCg                                                                                                                                           >  1:88805/1‑149 (MQ=255)
            gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                                                                         >  1:232692/1‑149 (MQ=17)
            gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGACCACACGGAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                                                                         >  1:445776/1‑149 (MQ=11)
             tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGCCGGATAAGGCGTTTACGCCCCATCCGACATCAATCCCTGATGCGACg                                                                                                                                        >  2:23705/1‑149 (MQ=255)
                   ccGCGTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCCCCTCCGACCTGACTCACTGCTGCGACGCTTTcc                                                                                                                                  >  2:17449/1‑149 (MQ=11)
                   ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGGTTTAGCGTGTTTTTGTAGGTCGGATAGTCCATTTCCGCCGCATCCGACATCAATACCTGTTACGACCCTTGcc                                                                                                                                  >  2:204678/1‑149 (MQ=11)
                     gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGACCTACGCCAGACAGCGCAAGAGCCTGATTTAGCGTGACTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACAATGCCTGATGCGACGCTTGCcgc                                                                                                                                >  1:827577/1‑149 (MQ=11)
                      cATCCGCCATCAACGCCTGATACGACGCTTCAAGAGTCTCGAACCGGCTACCCCTGACTGCGCAATACCCTAATTGATCGCGAATGTGTAAGTCGCATAAAGCGTGAACGCCGCGTCCGACATCAACGCCTGATGCGACGCTTGCcgcg                                                                                                                               >  2:183151/1‑149 (MQ=2)
                       aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                              >  2:314029/1‑149 (MQ=25)
                           gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCACTCGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                          >  1:677334/1‑149 (MQ=255)
                           gACATCAACGCCTGATGCGACGCTTAACGAGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCCCCTCTTa                                                                                                                          >  1:773187/1‑149 (MQ=255)
                                cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                                     <  1:757858/149‑1 (MQ=39)
                                     ccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                                                                                >  2:847851/1‑149 (MQ=37)
                                      cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACg                                                                                                               >  1:178301/1‑149 (MQ=39)
                                      cTGATGCGACGCTTAACGCGTCTCATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc                                                                                                                                                   >  2:132173/1‑113 (MQ=17)
                                      cTGATGCGACGCTTAACGCGTCTCATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc                                                                                                                                                   <  1:132173/113‑1 (MQ=18)
                                         aggCGACGCGTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa                                                                                                            <  1:625395/147‑1 (MQ=255)
                                         aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta                                                                                                            >  2:812255/1‑144 (MQ=9)
                                                 cTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                                                  >  1:462335/1‑135 (MQ=40)
                                                 cTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                                                  <  2:462335/135‑1 (MQ=40)
                                                     tcgtttgttattaGGCTTACGCGAGGACCCGCAATAGCCTCATTTACCGTGATTTTGTAGGCCGGATAAGCGGTTTACCCCGCTCACGACATCAACGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                                                <  2:108884/142‑1 (MQ=2)
                                                       gcgTCTTATGAGGCCTACGCGAGGGACCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCACCCGGCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                              <  2:138869/149‑1 (MQ=11)
                                                        cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                    <  1:586460/126‑1 (MQ=40)
                                                        cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                    >  2:586460/1‑126 (MQ=40)
                                                        cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                                                                             >  1:534788/1‑149 (MQ=38)
                                                          tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                                                                           >  2:685200/1‑149 (MQ=37)
                                                           cTTATCAGCCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGa                                                                                          <  1:159116/149‑1 (MQ=255)
                                                           cTTAGCAGGCCGACGCCAACAAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGa                                                                                          <  2:88805/149‑1 (MQ=14)
                                                               aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc                                                                                                      >  2:750375/1‑134 (MQ=18)
                                                               aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc                                                                                                      <  1:750375/134‑1 (MQ=40)
                                                                tgatccgtACGGCAGAGAGCGCAAGAGCCTGAGTGAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCAGCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa                                                                                      <  2:791393/142‑1 (MQ=11)
                                                                  aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa                                                                                                                                               <  2:105987/90‑1 (MQ=18)
                                                                  aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                                                                                                                               >  1:105987/1‑90 (MQ=33)
                                                                  aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                                    <  1:847851/149‑1 (MQ=39)
                                                                  aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                                    <  2:534788/149‑1 (MQ=38)
                                                                  aGGCCTACGCCAGACAGCGCAAGAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGAGTATTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                                    <  1:183151/149‑1 (MQ=255)
                                                                   ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                    >  2:188325/1‑116 (MQ=12)
                                                                   ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                    <  1:188325/116‑1 (MQ=25)
                                                                       tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTGAt                                                                               >  1:522211/1‑149 (MQ=37)
                                                                            cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg                                                                                     <  1:391275/138‑1 (MQ=40)
                                                                            cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg                                                                                     >  2:391275/1‑138 (MQ=39)
                                                                            cAGACAGCGAAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                          <  1:581235/149‑1 (MQ=255)
                                                                              gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAg                                                                        <  1:809674/149‑1 (MQ=38)
                                                                                         aggcggATTTTGCGTCATTATGTAGGGCGGATCAGGCGTTTACGCCGCATACGACATCAACGCCTGATGGGACGCTTGCCGTGTCTTATCAGGCCTACGTTAGACAGCGCAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAgg                                                             <  2:819217/144‑1 (MQ=11)
                                                                                          gCCTGATTTAGCGTGATTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGc                                                            <  1:904321/149‑1 (MQ=255)
                                                                                            cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa                                                                                      >  1:268922/1‑121 (MQ=40)
                                                                                              gATTAAGCCTGGTTTTGTAGTAAGGTTAAGGCGTTTACGCGGCATCCGACGTCAACGCCTGATGCGGCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTCCGCGTGACTTTGTAGGTCGGATCAGGCCttt                                                        >  2:85034/1‑149 (MQ=11)
                                                                                               aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTACGCAAGCCAGCGCACTATCCTGCTTTAGCGTGCCTTCCTAGGTCAGCTACTGCGtttc                                                       >  2:820467/1‑148 (MQ=11)
                                                                                                tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGCCGTTTAt                                                      >  1:558530/1‑149 (MQ=255)
                                                                                                 ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATg                                                     <  2:677334/149‑1 (MQ=38)
                                                                                                   aGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                          >  1:591707/1‑126 (MQ=39)
                                                                                                       tGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                         <  1:430829/75‑1 (MQ=255)
                                                                                                       tGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                         >  2:430829/1‑75 (MQ=255)
                                                                                                             gtgagcgcGGATAAGGCATCTCCGCCGCTTCCCATGTAAACGCCTGATGCGACGCTTGCCGCCTCTTATCAGGCCTACGCCAGACATCGCAATAGCCTGATTTAGCGTGATTTTCTAGGTCCAATAAGGCCTTTATGCCGCATCCGACa                                         <  2:265658/142‑1 (MQ=11)
                                                                                                              gTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                       <  1:273399/70‑1 (MQ=255)
                                                                                                              gTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                                                                                       >  2:273399/1‑70 (MQ=255)
                                                                                                                  gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGCTCGGACAAGGCGTTTTTGCCGGATCCGCGATCAAc                                    >  2:899828/1‑149 (MQ=14)
                                                                                                                    tcggaacggCGTTTACGCCGGACACGACGTGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc                                  <  2:251396/142‑1 (MQ=11)
                                                                                                                      gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACGGCGCAATATCCTGATTTATCTTGATTTTGTAGGTCGGCTAAGGCGTTTCTGCCGCATCCGACATCAACGCCt                                >  2:145974/1‑149 (MQ=14)
                                                                                                                        tAAGGCGGTTCCTCCGCTTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGGATCCGACATCAACGCCTGa                              <  2:773187/149‑1 (MQ=11)
                                                                                                                            gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                  <  1:560089/77‑1 (MQ=255)
                                                                                                                              gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACg                                   >  2:388036/1‑138 (MQ=40)
                                                                                                                              gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACg                                   <  1:388036/138‑1 (MQ=40)
                                                                                                                              gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAc                        >  1:595494/1‑149 (MQ=39)
                                                                                                                              gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGACAGCGCAATAGCCTGATTTAGCTTCATATTGTAGATCGGATACTGCGTTTAAGCCGCATCCGACATCAACGCCTGATGCGAc                        >  2:323004/1‑149 (MQ=14)
                                                                                                                                             gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                          >  2:705514/1‑84 (MQ=40)
                                                                                                                                             gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTa         >  2:627912/1‑149 (MQ=35)
                                                                                                                                               aaTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATc       <  1:640385/148‑1 (MQ=21)
                                                                                                                                                aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtt                                                         <  1:596093/98‑1 (MQ=37)
                                                                                                                                                   aaCGCCTGATGCGACGCTTGCCGCGTCCTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc   <  1:114760/149‑1 (MQ=17)
                                                                                                                                                    aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAGGCCTGATGCGACGCTTAACGCGTCTTATCAGGcc  >  2:759976/1‑149 (MQ=14)
                                                                                                                                                     |                                                                                                                                                   
GTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCC  >  NC_000913/4295912‑4296207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: