New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 454260629 (0.600)8 (0.180) 8/272 4.8 19.5% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913_3_pae_tpiA = 4542902 39 (0.860)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGG  >  NC_000913_3_pae_tpiA/4542754‑4543049
                                                                                                                                                    |                                                                                                                                                   
ttCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCAGCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGGTTTCCTCTATGAGTCaaaa                                                                                                                                                     <  2:717949/149‑1 (MQ=255)
 tCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAAt                                                                                                                                                    <  1:738784/149‑1 (MQ=255)
      ggCTCGACGTATCTTCCTCATTATTCCATCCAAAAACGACATCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACATATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGccc                                                                                                                                               <  2:406905/149‑1 (MQ=255)
            aCGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCACCAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATg                                                                                                                                         >  1:232026/1‑149 (MQ=255)
               cATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCAATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATAttt                                                                                                                                      >  2:567823/1‑149 (MQ=255)
                aTCTTCCTCATTCTTCTCTCCAAAACCCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTc                                                                                                                                     <  1:839764/149‑1 (MQ=255)
                      cTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATAAGTAAAAATGGCCCCAAATGTTTCATtttt                                                                                                                               >  1:721414/1‑149 (MQ=255)
                             ttctCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGACCCAAATGTTTCATCTTTTGGGGGa                                                                                                                        >  1:787436/1‑149 (MQ=255)
                                  tcCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACt                                                                                                                   >  1:600452/1‑149 (MQ=255)
                                  tcCAAAAACCAACTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACt                                                                                                                   >  1:520222/1‑149 (MQ=255)
                                     aaaaaCAACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACtgtg                                                                                                                <  2:448590/149‑1 (MQ=255)
                                       aaaCCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCa                                                                                                              >  1:743476/1‑149 (MQ=255)
                                            accTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGtt                                                                                                         <  2:695106/149‑1 (MQ=255)
                                                cATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCa                                                                                                     <  1:781144/149‑1 (MQ=255)
                                                      atatAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCaaa                                                                                               >  2:115968/1‑149 (MQ=255)
                                                          aaaCATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCg                                                                                           >  1:122166/1‑149 (MQ=255)
                                                                   tgaaaaaagaaagCAAGAAAATACAAAGCAAACAATACAACTGAAAAAGTTTGTCTGCGATGCTTGCATCTCTGAGTAAAAATTGCCCTAAATGTTTCATCTTTTGGGGTAAACCTGTGCAGTGTTGGCAGTAAAACTCATTTacaaac                                                                                  <  2:248657/144‑2 (MQ=255)
                                                                   taaataaaGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaaaa                                                                                  <  2:549034/149‑1 (MQ=255)
                                                                   taaataaaGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaaaa                                                                                  >  2:63145/1‑149 (MQ=255)
                                                                      ataaaGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaaaacaa                                                                               >  2:485418/1‑149 (MQ=255)
                                                                       taaaGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTacaaaacaaa                                                                              >  1:272930/1‑149 (MQ=255)
                                                                             caaCAATATGATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGGGATGCTTTCCTTTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTTGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTTGGCTACAAAACAAAGTGTAc                                                                        <  2:101271/148‑1 (MQ=255)
                                                                               aCAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAg                                                                      <  1:9295/149‑1 (MQ=255)
                                                                                 aaTAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAgtgtcacgaa                                                                    >  2:286805/1‑143 (MQ=255)
                                                                                 aaTAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAATTGTACAGaa                                                                    >  1:826855/1‑149 (MQ=255)
                                                                                 aaTAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATAAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGac                                                                    >  2:79075/1‑148 (MQ=255)
                                                                                  agaGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAAc                                                                   <  1:337403/147‑1 (MQ=255)
                                                                                      aaTATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGACAGGCGATCGCGTTTACAAAACAAAGTGTACAGAACGACt                                                               <  1:416348/149‑1 (MQ=255)
                                                                                             aGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTATATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGAAAAACTGTGCAGTGTTGCGAGTAAAAGTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCagg                                                        >  1:281845/1‑147 (MQ=255)
                                                                                                caacaaATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACACCATGTAGAGAAGCACTGACCacctcg                                                     >  1:367113/1‑144 (MQ=255)
                                                                                                       tAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGa                                              <  1:749030/149‑1 (MQ=255)
                                                                                                             gAAAAAGTTTGTCTGCGATGCTTTCCTCCCGGAGTCAAAATGGCTCCAACTTTTTCATCTTTTGGGGGAAAACGGTGCAGTGTTGACCGTCAAAGTCGTTTCCACAAGAAAGTGTACGGAACGGATGCCCATGTCGAATTCGATGTAGt                                        >  2:708088/1‑149 (MQ=255)
                                                                                                              aaaaaGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTGTTGGGGGAACACTGTGCAGCGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGGTTTAGAAACAGtt                                       >  2:224311/1‑149 (MQ=255)
                                                                                                                             gATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGGTTTTTTAAAGGAAagc                        >  2:544124/1‑149 (MQ=255)
                                                                                                                             gATGCGTTCCTCTATGAGTCAAAATGGCCCCAACTGTTTCATCTTTTGGGGGAAAACTGTGCAGGGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAagc                        <  2:45257/149‑1 (MQ=255)
                                                                                                                                  tttCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCAt                   >  2:683553/1‑149 (MQ=255)
                                                                                                                                      ctctATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGaaa               >  1:686339/1‑149 (MQ=255)
                                                                                                                                       tctATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGGAAACTGTGCAGTATAGGCGGTCGAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGaaaa              >  1:163913/1‑149 (MQ=255)
                                                                                                                                       tctATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTATATGAAAAGGAAAGCAGCATGaaaa              >  1:778005/1‑149 (MQ=255)
                                                                                                                                         tATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAAtt            >  2:236527/1‑149 (MQ=255)
                                                                                                                                         tATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGAATGCCCATGTAGATTTAGAATTAGTTTTTTGAAAGGCAAACAGCATGAAAAtt            >  2:236471/1‑149 (MQ=255)
                                                                                                                                                   aaTGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTgg  <  1:481862/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCCAACAAATAAACTGAAAAAGTTTGTCCGCGATGCTTTCCTCTATGAGTCAAAATGGCCCCAAATGTTTCATCTTTTGGGGGAAAACTGTGCAGTGTTGGCAGTCAAACTCGTTTACAAAACAAAGTGTACAGAACGACTGCCCATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGAAAATTAAAACTCTGG  >  NC_000913_3_pae_tpiA/4542754‑4543049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.