New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	2729443 =	36 (0.390)	25 (0.290)	23/230	4.9	32.6%	noncoding (2/76 nt)	gltW	tRNA‑Glu
	?	NC_000913_3_pae_tpiA	= 4168247	70 (0.820)					intergenic (+131/‑41)	rrsB/gltT	16S ribosomal RNA of rrnB operon/tRNA‑Glu
Rejected: Coverage evenness skew score above cutoff.

TCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAAT  >  NC_000913_3_pae_tpiA/2729302‑2729581                                                                                                                                              |                                                                                                                                           tCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGa                                                                                                                                            >  1:936940/1‑142 (MQ=255) tCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGa                                                                                                                                            <  2:936940/142‑1 (MQ=255) tCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGaaaa                                                                                                                                    >  4:76415/1‑150 (MQ=255)   aaaCAAGACTTAACAGTCTGTTTTGAGATATTGAGGACGGCGACTTTCCCTCACAAACCAGCACGTGGCCACCCCTAAGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAAtt                                                                                                                                  <  1:231593/150‑1 (MQ=17)     aCAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGc                                                                                                                                >  1:832145/1‑150 (MQ=255)      cAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTCTCACTCACAAACCAGCAAGTGGCGTCCCCTAAGGGATTCGACCCCCTGTTACCACAGTGAAAGGGCGGCGTCATGGGCCTCTAGACGGCGCGGACCCGAAAAATgaa                                                                                                                               >  2:46292/1‑148 (MQ=14)            ttAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCAc                                                                                                                         >  1:439641/1‑150 (MQ=40)            ttAACAGTCTGTTTGGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCGGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCAc                                                                                                                         >  4:196697/1‑150 (MQ=255)               aCAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCATATCAcgcg                                                                                                                      <  2:571842/150‑1 (MQ=255)                  ttCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTg                                                                                                                   <  1:72012/149‑1 (MQ=21)                  gTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTg                                                                                                                   <  1:72036/150‑1 (MQ=35)                       ttttGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGa                                                                                                              <  3:225228/150‑1 (MQ=32)                       ttttGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGa                                                                                                              >  3:657895/1‑150 (MQ=32)                         ttGAGATATTGAGGTCGGCGACTTTCACTCGCAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGata                                                                                                            <  1:1012562/150‑1 (MQ=17)                         ttGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGata                                                                                                            <  4:459272/150‑1 (MQ=32)                          tGAGATATTGAGGTCGGCGACTTTCACTCACAAGCCAGCAAGAAGCGTCTCCTATGTGATTCTATCCTCTGTTACCCTCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCAACGCGTGataa                                                                                                           <  1:46292/150‑2 (MQ=11)                          tGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGatat                                                                                                           >  1:1133887/1‑150 (MQ=32)                            agaTATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATAttt                                                                                                         >  2:683871/1‑150 (MQ=32)                                atTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCgt                                                                                                     >  2:692285/1‑150 (MQ=32)                                 ttGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGa                                                                                                                                            <  2:412041/110‑1 (MQ=32)                                 ttGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGa                                                                                                                                            >  1:412041/1‑110 (MQ=32)                                   gAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGc                                                                                                                                <  1:1110717/120‑1 (MQ=11)                                   gAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGc                                                                                                                                >  2:1110717/1‑120 (MQ=11)                                   gAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGtt                                                                                                                    <  3:303416/132‑1 (MQ=11)                                   gAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGtt                                                                                                                    >  4:303416/1‑132 (MQ=11)                                   gAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTa                                                                                                  >  2:470320/1‑150 (MQ=11)                                   gAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTa                                                                                                  <  1:470320/150‑1 (MQ=11)                                                                                                                ggCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTAt                     >  1:655339/1‑150 (MQ=31)                                                                                                                     tgtCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACt                <  2:832145/150‑1 (MQ=32)                                                                                                                      gtCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACtt               <  4:87356/150‑1 (MQ=32)                                                                                                                         cTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTtc            <  3:541300/150‑1 (MQ=34)                                                                                                                          tGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTtca           >  4:404173/1‑150 (MQ=34)                                                                                                                            ggCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTtcatc         <  1:692285/150‑1 (MQ=37)                                                                                                                              ccTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAg       >  2:472870/1‑150 (MQ=37)                                                                                                                               ctctAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGa      <  2:1055314/150‑1 (MQ=39)                                                                                                                                   aGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAAt  <  2:855043/150‑1 (MQ=39)                                                                                                                                              |                                                                                                                                           TCAAACAAGACTTAACAGTCTGTTTTGAGATATTGAGGTCGGCGACTTTCACTCACAAACCAGCAAGTGGCGTCCCCTAGGGGATTCGAACCCCTGTTACCGCCGTGAAAGGGCGGTGTCCTGGGCCTCTAGACGAAGGGGACACGAAAATTGCTTATCACGCGTTGCGTGATATTTTCGTGTAGGGTGAGCTTTCATTAATAGAAAGCGAACGGCCTTATTCTCTTCAGCCTCACTCCCAACGCGTAAACGCCTTGCTATTCACTTTTCATCAGACAAT  >  NC_000913_3_pae_tpiA/2729302‑2729581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.