New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	225455 =	12 (0.130)	21 (0.230)	20/250	6.3	56.0%	noncoding (75/77 nt)	ileV	tRNA‑Ile
	?	NC_000913_3_pae_tpiA	225478 =	21 (0.230)					intergenic (+21/‑22)	ileV/alaV	tRNA‑Ile/tRNA‑Ala
Rejected: Coverage evenness skew score above cutoff.

AAGTCCACTCAGGCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTC  >  NC_000913_3_pae_tpiA/225438‑225626                                          |                                                                                                                                                     aaGTCCACTCAGGCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTgg                                         >  1:111803/1‑150 (MQ=11)   gTCCACTCAGGCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGtt                                       <  2:111803/150‑1 (MQ=32)    tCCACTCAGGCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTta                                      <  1:33624/150‑1 (MQ=32)           aGGCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATaaaa                               <  4:696317/150‑1 (MQ=32)           aGGCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGAAACCGCATAGCTCCACCATCTCTGTAGTGGTTAAATaaaa                               <  2:312359/150‑1 (MQ=17)             gCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTtaaataaa                                <  4:563965/147‑1 (MQ=32)             gCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTtaaataaa                                >  3:563965/1‑147 (MQ=32)             gCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATaaaaaa                             <  1:1015231/150‑1 (MQ=32)               cTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTAAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGAAGTAAAATAAAAAATa                           <  2:859463/150‑1 (MQ=255)                tACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATAc                          >  1:1001613/1‑150 (MQ=32)                tACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATAc                          >  2:399253/1‑150 (MQ=32)                 ttcAAATTTGC‑CGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACt                         >  1:258455/3‑150 (MQ=255)                 aCCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACt                         >  3:506696/1‑150 (MQ=32)                  ccAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACtt                        >  1:671227/1‑150 (MQ=32)                   cAAATTTGCCGT‑GCAAATTTGAAGAGGTTTTAACTACATGTTCTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCCCATCTCAGTAGTGGTTAAATAAAAAATACTTc                       >  1:153155/1‑150 (MQ=11)                   cAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTc                       >  1:746235/1‑150 (MQ=32)                     aaTTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCag                     <  2:671227/150‑1 (MQ=32)                     aaTTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCag                     >  3:492585/1‑150 (MQ=32)                      aTTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATATCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCaga                    <  1:136449/150‑1 (MQ=17)                            aCG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTAc              <  4:492585/150‑1 (MQ=32)                                  aaaTTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCa         >  4:187025/1‑150 (MQ=32)                                     tttGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAg      <  2:258455/150‑1 (MQ=32)                                         aaGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTc  <  4:307570/150‑1 (MQ=32)                                          |                                                                                                                                                     AAGTCCACTCAGGCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTGTACCTGCAAAGGTTC  >  NC_000913_3_pae_tpiA/225438‑225626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.