Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 1,263,669 G→A intergenic (‑169/‑45) lolB ← / → hemA lipoprotein localization factor/glutamyl tRNA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,263,6690GA84.3% 205.6 / 27.8 83intergenic (‑169/‑45)lolB/hemAlipoprotein localization factor/glutamyl tRNA reductase
Reads supporting (aligned to +/- strand):  ref base G (8/5);  new base A (38/32);  total (46/37)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

ACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGAC  >  NC_000913_3_pae_tpiA/1263523‑1263812
                                                                                                                                                  |                                                                                                                                               
aCGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACaa                                                                                                                                              <  2:27633/150‑1 (MQ=255)
         ttATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTaa                                                                                                                                     >  1:509174/1‑150 (MQ=255)
                ccTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAAc                                                                                                                                    <  3:100080/144‑1 (MQ=255)
                ccTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAAc                                                                                                                                    >  4:100080/1‑144 (MQ=255)
                 cTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCa                                                                                                                             >  2:624110/1‑150 (MQ=255)
                     aTGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGt                                                                                                                         <  1:624110/150‑1 (MQ=255)
                            cGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGtatt                                                                                                                  >  3:689078/1‑150 (MQ=255)
                             gTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATACTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGtatta                                                                                                                 >  1:1083348/1‑150 (MQ=255)
                                     ccGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGc                                                                                                         <  2:509174/150‑1 (MQ=255)
                                     ccGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGc                                                                                                         <  2:573551/150‑1 (MQ=255)
                                       gCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAg                                                                                                       >  2:439003/1‑150 (MQ=255)
                                        cTATCGTCTATGTTCAAGTTGTCTTAACTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGCGCGCTCTCTTTTATTGATCGTCCGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAgc                                                                                                      <  2:615234/150‑2 (MQ=255)
                                             gTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGa                                                                                                 >  3:327449/1‑150 (MQ=255)
                                              tCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGAc                                                                                                >  4:288292/1‑150 (MQ=255)
                                                    ttCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCtttt                                                                                          >  4:269180/1‑150 (MQ=255)
                                                    ttCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCtttt                                                                                          >  2:430088/1‑150 (MQ=255)
                                                    ttCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCtttt                                                                                          >  2:880722/1‑150 (MQ=255)
                                                       aaGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGc                                                                                       >  2:280013/1‑150 (MQ=255)
                                                              cTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCttt                                                                                           <  4:487225/139‑1 (MQ=255)
                                                              cTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCttt                                                                                           <  4:487221/139‑1 (MQ=255)
                                                              cTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCttt                                                                                           >  3:487221/1‑139 (MQ=255)
                                                              cTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCttt                                                                                           >  3:487225/1‑139 (MQ=255)
                                                                   ttGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGc                                                                                       >  3:477524/1‑138 (MQ=255)
                                                                   ttGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGc                                                                                       <  4:477524/138‑1 (MQ=255)
                                                                     gCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAAcc                                                                         >  2:55624/1‑150 (MQ=255)
                                                                      ccAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAAc                                                                                                                           <  1:28288/99‑1 (MQ=255)
                                                                      ccAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAAc                                                                                                                           >  2:28288/1‑99 (MQ=255)
                                                                      ccAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCa                                                                        >  1:1058788/1‑150 (MQ=255)
                                                                       cAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCAt                                                                       >  2:149998/1‑150 (MQ=255)
                                                                         gAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCg                                                                                                          >  1:1081216/1‑113 (MQ=255)
                                                                         gAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCg                                                                                                          <  2:1081216/113‑1 (MQ=255)
                                                                          aaTCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAg                                                                                        <  3:519659/130‑1 (MQ=255)
                                                                          aaTCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAg                                                                                        >  4:519659/1‑130 (MQ=255)
                                                                              tAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACgg                                                                >  1:107722/1‑150 (MQ=255)
                                                                               aaCGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGc                                                               >  1:791369/1‑150 (MQ=255)
                                                                                aCGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCa                                                              >  1:1150205/1‑150 (MQ=255)
                                                                                     tttCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGt                                                                                                                         >  1:962867/1‑86 (MQ=255)
                                                                                     tttCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGt                                                                                                                         <  2:962867/86‑1 (MQ=255)
                                                                                          gCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCtttt                                                                                          >  4:672525/1‑112 (MQ=255)
                                                                                          gCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCtttt                                                                                          <  3:672525/112‑1 (MQ=255)
                                                                                          gCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAAc                                                                          <  1:219824/128‑1 (MQ=255)
                                                                                          gCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAAc                                                                          >  2:219824/1‑128 (MQ=255)
                                                                                            aaTTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGCCATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTAt                                                       <  2:348492/145‑1 (MQ=255)
                                                                                            aaTTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTAt                                                       >  1:348492/1‑145 (MQ=255)
                                                                                             aTTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGc                                                 <  1:430088/150‑1 (MQ=255)
                                                                                                  tCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATc                                                                                                                              <  3:93280/68‑1 (MQ=255)
                                                                                                  tCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATc                                                                                                                              >  4:93280/1‑68 (MQ=255)
                                                                                                  tCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGaa                                            >  4:56096/1‑150 (MQ=255)
                                                                                                    cAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTa                                                                                         <  1:107653/103‑1 (MQ=255)
                                                                                                    cAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTa                                                                                         >  2:107653/1‑103 (MQ=255)
                                                                                                    cAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCt                                                           <  1:1111670/133‑1 (MQ=255)
                                                                                                    cAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCt                                                           >  2:1111670/1‑133 (MQ=255)
                                                                                                    cAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACg                                          >  4:218780/1‑150 (MQ=255)
                                                                                                     aaaaGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACgt                                         >  3:684641/1‑150 (MQ=255)
                                                                                                       aaGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACgtgt                                       <  1:55624/150‑1 (MQ=255)
                                                                                                                ctctctTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTc                              >  4:579536/1‑150 (MQ=255)
                                                                                                                   tctTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGAc                                                                                                     >  2:268576/1‑76 (MQ=255)
                                                                                                                   tctTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGAc                                                                                                     <  1:268576/76‑1 (MQ=255)
                                                                                                                   tctTTTATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGcc                           <  3:56096/150‑1 (MQ=255)
                                                                                                                     ttttATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCgg                         <  1:439003/150‑1 (MQ=255)
                                                                                                                     ttttATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCgg                         >  3:83162/1‑150 (MQ=255)
                                                                                                                      tttATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGa                        <  4:83162/150‑1 (MQ=255)
                                                                                                                      tttATTGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGa                        <  3:288292/150‑1 (MQ=255)
                                                                                                                         aTTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATaa                     <  1:280013/150‑1 (MQ=255)
                                                                                                                           tGATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGc                   >  2:656365/1‑150 (MQ=255)
                                                                                                                            gATCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGTATAAGCt                  <  2:1150205/150‑1 (MQ=255)
                                                                                                                             aTCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGtatt                                                                                                                  >  1:384671/1‑53 (MQ=255)
                                                                                                                             aTCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGtatt                                                                                                                  <  2:384671/53‑1 (MQ=255)
                                                                                                                             aTCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCAc                                                             >  3:11107/1‑106 (MQ=255)
                                                                                                                             aTCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCAc                                                             <  4:11107/106‑1 (MQ=255)
                                                                                                                             aTCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGc                                                 <  3:328560/118‑1 (MQ=255)
                                                                                                                             aTCTTACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGc                                                 >  4:328560/1‑118 (MQ=255)
                                                                                                                                ttACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGAt              >  1:895026/1‑150 (MQ=255)
                                                                                                                                 tACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATc             <  2:1083348/150‑1 (MQ=255)
                                                                                                                                 tACGCATCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATc             <  1:656365/150‑1 (MQ=255)
                                                                                                                                    gCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAgg          >  2:1045254/1‑150 (MQ=255)
                                                                                                                                     cATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGgc         <  1:1045254/150‑1 (MQ=255)
                                                                                                                                      aTCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACa                                                                                                    <  1:895846/58‑1 (MQ=255)
                                                                                                                                      aTCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACa                                                                                                    >  2:895846/1‑58 (MQ=255)
                                                                                                                                      aTCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGgcg        >  1:916511/1‑150 (MQ=255)
                                                                                                                                       tCCTGTATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGgcgc       <  3:218780/150‑1 (MQ=255)
                                                                                                                                            tATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGAc  >  2:804137/1‑150 (MQ=255)
                                                                                                                                            tATGATACAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGAc  <  2:791369/150‑1 (MQ=255)
                                                                                                                                               gataCAAGCAGACTAACCCTATCAACGTTGGTATTATTTc                                                                                                             >  1:893344/1‑40 (MQ=255)
                                                                                                                                               gataCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATc             >  3:44864/1‑136 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ACGTTGCAGTTATAACCCTTAATGCTAGCGTTACCGTCCGCTATCGTCTATGTTCAAGTTGTCTTAATTGCCAGAATCTAACGGCTTTCGGCAATTACTCCAAAAGGGGGCGCTCTCTTTTATTGATCTTACGCATCCTGTATGATGCAAGCAGACTAACCCTATCAACGTTGGTATTATTTCCCGCAGACATGACCCTTTTAGCACTCGGTATCAACCATAAAACGGCACCTGTATCGCTGCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGAC  >  NC_000913_3_pae_tpiA/1263523‑1263812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: