New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 4115916	43 (1.530)	4 (0.150)	4/276	3.4	9.0%	coding (1223/1509 nt)	glpK	glycerol kinase
	?	NC_000913_3_pae_tpiA	= 4115921	39 (1.440)					coding (1218/1509 nt)	glpK	glycerol kinase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCC‑ACGAGTCAGACCGAAAATCGCCCCG  >  NC_000913_3_pae_tpiA/4115768‑4116053                                                                                                                                                     |                                                                                                                                           gTTCTGCCAGAAGCGGAGCGCCGGGCTTGCGGGATAGGCGGCATCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCATCGCGGGTGCCGAGCATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTTCGCCACCATCCAcgc                                                                                                                                            <  2:408291/149‑1 (MQ=255)     tGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTCACTTCGCGCACTTCCGGGCGCTCAACGCGGGAGCCGAGAATAACGGACAGGAACCGCCACAGGAAATTGTTTGCTACTGCGCCACCATCCCCGCCCAg                                                                                                                                        >  2:413333/1‑149 (MQ=255)        aaGAAGCCTACCGCCAGGGCGGCGAGATAGGCCGCACCCTATGTGGTGACTTCGCGCATTTCCGGGCTCTCAACGCTGTGGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGc                                                                                                                                     <  2:97721/148‑1 (MQ=255)         aGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCCGGCTGCTTGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCg                                                                                                                                    >  1:468501/1‑149 (MQ=255)               cAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCGGa                                                                                                                              >  1:273733/1‑149 (MQ=255)               cAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGAAGa                                                                                                                              >  2:298253/1‑149 (MQ=255)                caCCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATACCGGACTGGGAGTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGAc                                                                                                                             <  2:182907/148‑1 (MQ=255)                aaCCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGAc                                                                                                                             <  1:364278/149‑1 (MQ=255)                aaCCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCCCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGCAATTGTTTGCTACTGCGCCCCCATCCCCGCGCAGGGCGTGCAGAc                                                                                                                             >  1:225636/1‑149 (MQ=255)                    gCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGAt                                                                                                                         >  1:413462/1‑149 (MQ=255)                    gCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACGCCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGAt                                                                                                                         <  1:92595/149‑1 (MQ=255)                     ccAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGAATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGCCGCAtc                                                                                                                        >  1:172092/1‑148 (MQ=255)                       aGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATAcc                                                                                                                      >  2:358981/1‑149 (MQ=255)                        ggCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTGTCGCACTGCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCa                                                                                                                     <  1:386752/149‑1 (MQ=255)                        cgCCTGCGAGAGCGGGCGCACCCAATGCGGTGACTTCGCTCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCa                                                                                                                     <  2:349929/148‑1 (MQ=255)                            tGCGAGATAGGCCGGCCGCCGTGCGGTGACTTCGCGCACCGCCGGGCGGTGAACGCGGGTGGCGAGAATATCGGACGGGAACTGCAGCAGGAAAGTGTTTGCTAATGCGCCACCATCCACGCGCATGGGGTGCAGACGGATACCAGAGt                                                                                                                 <  2:58099/149‑1 (MQ=255)                             gCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCCCTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACGTGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTc                                                                                                                <  1:194941/149‑1 (MQ=255)                                  aTAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTACCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGCGCAGACGGATACCAGAGTCGGCCt                                                                                                           <  1:429339/149‑1 (MQ=255)                                     ggCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCa                                                                                                        <  1:83681/149‑1 (MQ=255)                                       ccGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATc                                                                                                      <  2:439389/149‑1 (MQ=255)                                         gCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGc                                                                                                    <  2:59392/149‑1 (MQ=255)                                           aCCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCCTCGCtt                                                                                                  >  2:455523/1‑149 (MQ=255)                                               aaTGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCa                                                                                                                                         >  1:462135/1‑106 (MQ=255)                                               aaTGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCa                                                                                                                                         <  2:462135/106‑1 (MQ=255)                                                  gCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCAc                                                                                           <  2:41192/149‑1 (MQ=255)                                                  agggTGACTTCCCGCACTTCCGGGCGCTCAACGAGGGTGTCGAGAATATCGGACTGGAACTGCATCAGGAAATCGTTTGCTACTGCGCCACCATCCCCGCGCGGGGCGTGCAGACTGATACCAGAGTCGGCCTGCATCGCTTCCAGCAc                                                                                           <  2:41176/147‑1 (MQ=255)                                                     gTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATCGCAGAGTGGGCCTGCATCGCTTCCAGCACGTc                                                                                        >  2:212970/1‑149 (MQ=255)                                                       gaattCGCCCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTTCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCAc                                                                                      <  2:363926/146‑1 (MQ=255)                                                             gcgcACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCt                                                                                >  1:350623/1‑149 (MQ=255)                                                                      cggcggcTCAGCGCGGCTGCCGAGAATAGCGGACTGGAACTTCATCAGGAAATTGTTGGCTACTGCGCCACCATCCACGCTCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCaa                                                                       <  2:68361/144‑1 (MQ=255)                                                                           gcTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGAc                                                                  <  1:478912/149‑1 (MQ=255)                                                                             tCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCATGCAGGTGGAGACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTc                                                                >  1:366046/1‑149 (MQ=255)                                                                                              aaTATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCAGCAGCCACGCGCAGGGCGTGCCAAAGGATACCAGGGCGTTTTTTCGTGGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATaa                                               <  1:243436/149‑1 (MQ=255)                                                                                                    ggACTGGAACTGCATCCGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGt                                         <  1:87079/149‑1 (MQ=255)                                                                                                    ggACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGt                                         <  1:86983/149‑1 (MQ=255)                                                                                                      aCTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGAAATAGACTCCAGCGTCGCGCGCATAAGGTGGGTa                                       >  2:464618/1‑149 (MQ=255)                                                                                                        tGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCGCAATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGACCGCTAAGCAATAGACTCCCGCGTCGCGCGTATAATGAGGTTAGc                                     >  1:218809/1‑149 (MQ=255)                                                                                                                   cAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCCTATAATGTGGTTAGCGTTCACCCC‑Ac                         <  2:120487/149‑1 (MQ=255)                                                                                                                                   aCTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGCATAACTTGGTTAGCGTTCACCCCAACGA‑TCAAACAGAAAAt         >  2:64245/1‑149 (MQ=255)                                                                                                                                     tGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCC‑ACGAGTCAGACCGAAAATCg       <  2:174111/149‑1 (MQ=255)                                                                                                                                          caccaTCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCC‑ACGAGTCAGACCGAAAATCGCCCcg  >  2:481096/1‑149 (MQ=255)                                                                                                                                          caccaTCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCC‑ACGAGTCAGACCGAAAATCGCCCcg  >  1:89648/1‑149 (MQ=255)                                                                                                                                          caccaTCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGCCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACCCCAGCGTCGCGCGTATAAGGTGGGTAACGTTCACCCC‑ACGAGACCGCCCGCAAACCGcccca  >  1:263580/1‑148 (MQ=255)                                                                                                                                               tCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCC‑ACGAGTCAGAcc               >  2:151097/1‑131 (MQ=255)                                                                                                                                               tCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCC‑ACGAGTCAGAcc               <  1:151097/131‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                           GTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCC‑ACGAGTCAGACCGAAAATCGCCCCG  >  NC_000913_3_pae_tpiA/4115768‑4116053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.