New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	4216179 =	24 (0.850)	4 (0.150)	4/274	3.4	14.8%	coding (786/1602 nt)	aceB	malate synthase A
	?	NC_000913_3_pae_tpiA	4216194 =	23 (0.850)					coding (801/1602 nt)	aceB	malate synthase A
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTGGCAGGAAGCGGCCTGGTGGAGCGAAGTCTTCAGCTATGCAGAAGATCGCTTTAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGA  >  NC_000913_3_pae_tpiA/4216047‑4216339                                                                                                                                                    |                                                                                                                                                  ttGGCAGGAAGCGGCTTGGTGCAGCGAAGTATTCAGCTATGCAGAGGATCGCTTTAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGAcc                                                                                                                                                  <  2:205334/148‑1 (MQ=255)            cGGCCTGGTGGAGCGAAGTCTTCAGCTATGCAGAAGATCGCTTTAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGt                                                                                                                                       <  1:399220/149‑1 (MQ=255)                cTGGTGGAGCGAAGTCTTCAGCTATGCAGAAGATCGCTTTAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGa                                                                                                                                   >  2:58205/1‑149 (MQ=255)                    tcgAGCGAAGTCTTCAGCTAGGCAGAAGATCGCTTTAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTg                                                                                                                               <  1:475717/147‑1 (MQ=255)                            aGTCTTCAGCTATGCAGAAGATCGCTTTAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGtt                                                                                                                       <  1:286663/149‑1 (MQ=255)                                ttCAGCTATGCAGAAGACCGCTTTAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGa                                                                                                                   <  2:184205/149‑1 (MQ=255)                                 gcAGCGATGCAGAAGGTCGCTTTAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGAt                                                                                                                  <  2:249532/148‑1 (MQ=255)                                           agaagaTCCCTTTAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCa                                                                                                        <  1:162499/149‑1 (MQ=255)                                                 tCGCTTTAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCtata                                                                                                  <  1:408545/149‑1 (MQ=255)                                                     tttAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCaa                                                                                              >  2:460920/1‑149 (MQ=255)                                                     tttAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCaa                                                                                              >  1:253455/1‑149 (MQ=255)                                                                          aTCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCTGGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAAGTATCCCGa                                                                         >  2:173515/1‑149 (MQ=255)                                                                          aTCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGa                                                                         <  1:58205/149‑1 (MQ=255)                                                                                      ttGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTCAAAAACTATCCCGATCACGTCCTccc                                                             >  2:23626/1‑149 (MQ=255)                                                                                         cTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCcaga                                                          <  1:23626/149‑1 (MQ=255)                                                                                               gAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAgacagaca                                                    <  1:155072/149‑1 (MQ=255)                                                                                                     cTGCCCGCCGGGTTCCAGATGGATGAAATCCTTCACGCGTTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGt                                              <  2:478412/149‑1 (MQ=255)                                                                                                             cgTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATgg                                      >  1:33610/1‑149 (MQ=255)                                                                                                                        tggatgAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGAGCGCTTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTc                           <  2:253815/149‑1 (MQ=255)                                                                                                                               aaTCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATg                    >  1:421563/1‑149 (MQ=255)                                                                                                                               aaTCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATg                    >  1:166344/1‑149 (MQ=255)                                                                                                                                          cgcTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCca                                                            <  2:314456/98‑1 (MQ=255)                                                                                                                                          cgcTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCca                                                            >  1:314456/1‑98 (MQ=255)                                                                                                                                               cGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGtt    <  1:242820/149‑1 (MQ=255)                                                                                                                                               cGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGtt    <  2:33610/149‑1 (MQ=255)                                                                                                                                                 tGATCATATTGTTGGTCTGAACGGCGGGCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCCGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGttta  <  2:253455/149‑3 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CTGGCAGGAAGCGGCCTGGTGGAGCGAAGTCTTCAGCTATGCAGAAGATCGCTTTAATCTGCCGCGCGGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAAATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGA  >  NC_000913_3_pae_tpiA/4216047‑4216339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.