Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,158,704 C→A T100K (ACA→AAA)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,158,7040CA97.1% 491.5 / 4.3 139T100K (ACA→AAA) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (68/67);  total (70/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 139 total aligned reads displayed.

CCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTG  >  NC_000913_3_pae_tpiA/4158557‑4158849
                                                                                                                                                   |                                                                                                                                                 
ccAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAg                                                                                                                                                 <  1:1378615/150‑1 (MQ=255)
 cAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTACACATTGGTTTGATTCCCAAAGt                                                                                                                                                >  4:702034/1‑150 (MQ=255)
  aGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGtt                                                                                                                                               >  1:1073670/1‑150 (MQ=255)
  aGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGtt                                                                                                                                               >  1:451532/1‑150 (MQ=255)
  aGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTACACATTGGTTTGATTCCCAAAGtt                                                                                                                                               <  3:702034/150‑1 (MQ=255)
    ccGTACATTGTTGTTCACCCATGCTGGAATGCTGCTTGTGGATCAGGGGCGTACCGCGCGGCGTGAGGGGAAGGGCCTTAAAGAGATGGCCAGCCAGCAGGGCGAGACAATGAACGAACCGCTGCACATTGGTTTGATTCCCAAAGTTgg                                                                                                                                             >  2:1057589/1‑150 (MQ=255)
    ccGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTgg                                                                                                                                             >  2:1526517/1‑150 (MQ=255)
     cGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTCGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGa                                                                                                                                            >  2:499887/1‑150 (MQ=255)
       tAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGAcc                                                                                                                                          <  3:145025/150‑1 (MQ=255)
        aaaGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTTCCAAAGTTGGACCg                                                                                                                                         >  1:1578267/1‑150 (MQ=255)
                gttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCt                                                                                                                                 >  2:1011106/1‑150 (MQ=255)
                gttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCt                                                                                                                                 >  2:429634/1‑150 (MQ=255)
                 ttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTa                                                                                                                                <  2:126948/150‑1 (MQ=255)
                   cACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTAcc                                                                                                                              >  3:357739/1‑150 (MQ=255)
                     cccAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAg                                                                                                                                                 >  4:659407/1‑129 (MQ=255)
                     cccAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAg                                                                                                                                                 <  3:659407/129‑1 (MQ=255)
                        aGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGCTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCata                                                                                                                         <  4:42545/150‑1 (MQ=255)
                        aGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCCGCACATTGGTTTGATTCCCAAAGTTGGATCGTACCTGCTACCGCata                                                                                                                         <  2:1018487/150‑1 (MQ=255)
                            gggAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCAtattat                                                                                                                     <  2:715115/150‑1 (MQ=255)
                            gggAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCAtattat                                                                                                                     >  1:882381/1‑150 (MQ=255)
                             ggAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATc                                                                                                                    >  1:782455/1‑150 (MQ=255)
                              gAATGCTGCTGGTGGATCAGGCGGGTACCGTGCTGCGTGAGGTGAAAGTCGTTAAAGGGATGGCAAGCCAGCAGGGCGAGACGTTGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                                   <  1:882310/150‑1 (MQ=255)
                              gAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCat                                                                                                                          >  2:903287/1‑143 (MQ=255)
                              gAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCat                                                                                                                          <  1:903287/143‑1 (MQ=255)
                                  gctgctGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGAACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTAt                                                                                                               <  1:579234/150‑1 (MQ=255)
                                      ctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctg                                                                                                           >  1:1394075/1‑150 (MQ=255)
                                         gtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGGCCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCg                                                                                                                             <  2:1090080/129‑1 (MQ=255)
                                         gtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCg                                                                                                                             >  1:1090080/1‑129 (MQ=255)
                                         gtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCAc                                                                                                        >  3:479848/1‑150 (MQ=255)
                                          tggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCAcc                                                                                                       >  2:1174764/1‑150 (MQ=255)
                                           ggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgc                                                                                                             <  1:664577/143‑1 (MQ=255)
                                           ggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgc                                                                                                             >  2:664577/1‑143 (MQ=255)
                                           ggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCa                                                                                                      <  4:479848/150‑1 (MQ=255)
                                              tCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGAc                                                                                                   >  1:1339521/1‑150 (MQ=255)
                                                aGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                                   >  2:471673/1‑132 (MQ=255)
                                                aGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                                   <  1:471673/132‑1 (MQ=255)
                                                  gcgcGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                               <  1:1011106/150‑1 (MQ=255)
                                                   cgcgTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTc                                                                                              <  1:1039120/150‑1 (MQ=255)
                                                   cgcgTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTc                                                                                              <  1:431747/150‑1 (MQ=255)
                                                    gcgTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTcc                                                                                             <  2:1394075/150‑1 (MQ=255)
                                                       tACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCaaa                                                                                          <  3:334605/150‑1 (MQ=255)
                                                        aCCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGt                                                                                                                                        >  1:1630599/1‑103 (MQ=255)
                                                        aCCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGt                                                                                                                                        <  2:1630599/103‑1 (MQ=255)
                                                          cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGGCCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                       <  3:609953/150‑1 (MQ=255)
                                                          cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                       <  1:1317440/150‑1 (MQ=255)
                                                          cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                       <  2:882381/150‑1 (MQ=255)
                                                          cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                       <  1:545732/150‑1 (MQ=255)
                                                          cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGATTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                       >  2:340547/1‑150 (MQ=255)
                                                            tgctgcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTgg                                                                                     <  2:97260/150‑1 (MQ=255)
                                                            tgctgcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTgg                                                                                     >  3:294673/1‑150 (MQ=255)
                                                             gctgcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGa                                                                                    <  2:451532/150‑1 (MQ=255)
                                                              ctgcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTcc                                                                                             >  1:306953/1‑140 (MQ=255)
                                                                   tGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCt                                                                                                                 >  3:532153/1‑115 (MQ=255)
                                                                    gAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                                                             >  1:909445/1‑150 (MQ=255)
                                                                        tGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGc                                                                         <  2:749378/150‑1 (MQ=255)
                                                                               ccTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGc                                                                  <  1:295154/150‑1 (MQ=255)
                                                                               ccTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGc                                                                  >  3:366148/1‑150 (MQ=255)
                                                                                 ttAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcac                                                                >  2:183005/1‑150 (MQ=255)
                                                                                 ttAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcac                                                                <  1:949115/150‑1 (MQ=255)
                                                                                   aaaGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAg                                                              >  2:1493944/1‑150 (MQ=255)
                                                                                       agaTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                                   >  2:288212/1‑93 (MQ=255)
                                                                                       agaTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                                   <  1:288212/93‑1 (MQ=255)
                                                                                       agaTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTc                                                                                              >  3:970382/1‑114 (MQ=255)
                                                                                           ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACccacca                                                      >  2:194296/1‑150 (MQ=255)
                                                                                             cAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTGTGATGCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGt                                                    >  2:476390/1‑150 (MQ=255)
                                                                                               aGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTa                                                  <  1:193404/150‑1 (MQ=255)
                                                                                                    gcagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTTCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGgc                                             <  2:1578267/150‑1 (MQ=255)
                                                                                                        ggCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCaa                                         >  2:1408685/1‑150 (MQ=255)
                                                                                                        ggCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCaa                                         >  3:542301/1‑150 (MQ=255)
                                                                                                          cGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTAcc                                                                                                                              >  1:597969/1‑63 (MQ=255)
                                                                                                           gagaCGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTg                                      <  2:782455/150‑1 (MQ=255)
                                                                                                            agaCGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTgg                                     <  1:652927/150‑1 (MQ=255)
                                                                                                             gaCGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGa                                    >  1:1504292/1‑150 (MQ=255)
                                                                                                             gaCGATGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGa                                    >  3:922264/1‑150 (MQ=255)
                                                                                                                  tGTCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCg                               <  1:1446951/150‑1 (MQ=255)
                                                                                                                    tCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGAc                                                                                                   >  4:327397/1‑80 (MQ=255)
                                                                                                                    tCCGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGAc                                                                                                   <  3:327397/80‑1 (MQ=255)
                                                                                                                     ccGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCa                                                                                            <  3:159765/86‑1 (MQ=255)
                                                                                                                     ccGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCa                                                                                            >  4:159765/1‑86 (MQ=255)
                                                                                                                      cGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCAc                                                                                                        >  2:278123/1‑73 (MQ=255)
                                                                                                                      cGGACCGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGtt                                                   >  2:1080697/1‑126 (MQ=255)
                                                                                                                          ccGCTGCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTc                       <  4:366148/150‑1 (MQ=255)
                                                                                                                               gCACATTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                                                             <  4:15578/91‑1 (MQ=255)
                                                                                                                                cacaTTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGc                 <  3:132577/150‑1 (MQ=255)
                                                                                                                                cacaTTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACGCGATTGc                 <  2:1076304/150‑1 (MQ=255)
                                                                                                                                cacaTTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTCGCGCAACTGGACAGCGGCAAACTCGATTGc                 <  4:188411/150‑1 (MQ=255)
                                                                                                                                 acaTTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAc                                                            <  2:1315433/106‑1 (MQ=255)
                                                                                                                                 acaTTGGTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAc                                                            >  1:1315433/1‑106 (MQ=255)
                                                                                                                                      ggTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGaa                                                                                   >  2:579876/1‑78 (MQ=255)
                                                                                                                                      ggTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATc           <  1:183005/150‑1 (MQ=255)
                                                                                                                                       gTTTGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATcc          >  1:1104209/1‑150 (MQ=255)
                                                                                                                                        tttGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTAc                                                 <  2:1307715/110‑1 (MQ=255)
                                                                                                                                          tGATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTcg       >  2:547074/1‑150 (MQ=255)
                                                                                                                                           gATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGt               <  2:1143245/141‑1 (MQ=255)
                                                                                                                                           gATTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGt               >  1:1143245/1‑141 (MQ=255)
                                                                                                                                            aTTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACccac                                                        >  1:741457/1‑99 (MQ=255)
                                                                                                                                            aTTCCCAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACccac                                                        <  2:741457/99‑1 (MQ=255)
                                                                                                                                               cccAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAc                                                            >  1:1605809/1‑92 (MQ=255)
                                                                                                                                               cccAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCa                            <  4:201633/124‑1 (MQ=255)
                                                                                                                                               cccAAAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCa                            >  3:201633/1‑124 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTG  >  NC_000913_3_pae_tpiA/4158557‑4158849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: