BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	1,800,841	T→G	K577T (AAG→ACG)	thrS ←	threonyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	1,800,841	0	T	G	100.0%	459.0 / NA	126	K577T (AAG→ACG)	thrS	threonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (66/60); total (66/60)

Only 100 of 129 total aligned reads displayed.

TCGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAA  >  NC_000913_3_pae_tpiA/1800694‑1800986

tcGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAAtcgtc                                                                                                                                                 <  1:176315/150‑1 (MQ=255)

tcGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAAtcgtc                                                                                                                                                 <  4:511913/150‑1 (MQ=255)

       cTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTtc                                                                                                                                          >  3:282134/1‑150 (MQ=255)

        ttCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTtct                                                                                                                                         >  1:197755/1‑150 (MQ=255)

        ttCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTtct                                                                                                                                         >  1:472578/1‑150 (MQ=255)

          cATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCa                                                                                                                                       <  1:1160840/150‑1 (MQ=255)

           aTTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCaa                                                                                                                                      <  2:139848/150‑1 (MQ=255)

                  tCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCt                                                                                                                               >  1:925667/1‑150 (MQ=255)

                   ccATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTCTCACAAAAGACCAGCCTATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCtt                                                                                                                              <  3:761255/150‑1 (MQ=255)

                    cATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCttt                                                                                                                             <  4:863688/150‑1 (MQ=255)

                     aTGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTa                                                                                                                            >  1:1491394/1‑150 (MQ=255)

                      tGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTaa                                                                                                                           >  2:957161/1‑150 (MQ=255)

                            ccAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGaa                                                                                                                     <  3:298737/150‑1 (MQ=255)

                              aGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGCGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATg                                                                                                                   >  4:766340/1‑150 (MQ=255)

                                gTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGcc                                                                                                                 <  3:159472/150‑1 (MQ=255)

                                gTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGcc                                                                                                                 >  4:629500/1‑150 (MQ=255)

                                 tCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGccc                                                                                                                >  1:81995/1‑150 (MQ=255)

                                    ttACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCa                                                                                                             <  1:742968/150‑1 (MQ=255)

                                      aCCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGc                                                                                                                  <  1:740521/143‑1 (MQ=255)

                                      aCCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGc                                                                                                                  >  2:740521/1‑143 (MQ=255)

                                      aCCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCAtt                                                                                                           >  1:4700/1‑150 (MQ=255)

                                      aCCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCAtt                                                                                                           <  2:472578/150‑1 (MQ=255)

                                      aCCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCAtt                                                                                                           <  4:587034/150‑1 (MQ=255)

                                       ccACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCAttt                                                                                                          <  1:440928/150‑1 (MQ=255)

                                           ggcggGTGCGAACGTCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATa                                                                                                      >  4:472256/1‑150 (MQ=255)

                                                 tGCGAACGGCGAATTTGCATGAGTAAACCTCTCTATCGCCACACCTCTGTCTCGATGTGACGCGACGAAAAGTGTGCTCGTGGATCTTAAAGCCAGTCGTCTCATTTCTCAAGTCTGCTGTAACACGAATGTCCGCATTTGATAGtgttt                                                                                                <  2:564758/150‑1 (MQ=255)

                                                 tGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGttttt                                                                                                <  1:1397082/150‑1 (MQ=255)

                                                  gCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTg                                                                                               >  2:1348409/1‑150 (MQ=255)

                                                   cGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGc                                                                                              <  3:513566/150‑1 (MQ=255)

                                                    gAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCttt                                                                                                                             >  3:334867/1‑118 (MQ=255)

                                                    gAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCtt                                                                                                                              <  1:353412/117‑1 (MQ=255)

                                                    gAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCtt                                                                                                                              >  2:353412/1‑117 (MQ=255)

                                                     aaCGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGt                                                                                            <  1:1204795/150‑1 (MQ=255)

                                                     aaCGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGt                                                                                            <  2:197755/150‑1 (MQ=255)

                                                      aCGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGtttt                                                                                                 <  1:578401/144‑1 (MQ=255)

                                                      aCGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGtttt                                                                                                 >  2:578401/1‑144 (MQ=255)

                                                        ggCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCaa                                                                                         >  2:921655/1‑150 (MQ=255)

                                                              tttGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCAttt                                                                                                                                           >  1:1161925/1‑94 (MQ=255)

                                                              tttGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCAttt                                                                                                                                           <  2:1161925/94‑1 (MQ=255)

                                                               ttGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTa                                                                                  <  3:766340/150‑1 (MQ=255)

                                                                 gCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAAc                                                                                <  2:1086704/150‑1 (MQ=255)

                                                                  ccTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCa                                                                                                             >  1:134938/1‑120 (MQ=255)

                                                                  ccTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCa                                                                                                             <  2:134938/120‑1 (MQ=255)

                                                                    tGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTa                                                                             >  1:1042139/1‑150 (MQ=255)

                                                                     gATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTAt                                                                            >  2:1611465/1‑150 (MQ=255)

                                                                        tCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCa                                                                         <  1:1488406/150‑1 (MQ=255)

                                                                          cACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGCTAGTTTTTGCGTCACTTCGTTAACGTATTCAGa                                                                       >  2:1223703/1‑150 (MQ=255)

                                                                          cACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGa                                                                       <  1:222335/150‑1 (MQ=255)

                                                                           aCCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGAc                                                                      >  3:43019/1‑150 (MQ=255)

                                                                             ctctTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGACAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTTCGTCAATTCGTTAACTTATTCAGACtt                                                                    >  2:1263220/1‑149 (MQ=255)

                                                                              tctTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACtgt                                                                   >  2:350117/1‑150 (MQ=255)

                                                                                  tATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAAt                                                               <  1:1257849/150‑1 (MQ=255)

                                                                                   aTCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATc                                                              <  1:921655/150‑1 (MQ=255)

                                                                                    tCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCg                                                             >  2:936222/1‑150 (MQ=255)

                                                                                     caccacAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGCACGCCCGCATGTAATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCgg                                                            <  2:1491394/150‑1 (MQ=255)

                                                                                       ccacAGACCAGCATATGTGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTa                                                          >  1:429305/1‑150 (MQ=255)

                                                                                       ccacAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTa                                                          <  4:282134/150‑1 (MQ=255)

                                                                                       ccacAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTa                                                          >  1:1198864/1‑150 (MQ=255)

                                                                                        cacaGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGa                                                                       <  2:1609060/136‑1 (MQ=255)

                                                                                        cacaGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGa                                                                       >  1:1609060/1‑136 (MQ=255)

                                                                                            gACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATAtt                                                     <  4:64797/150‑1 (MQ=255)

                                                                                                 gCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGa                                                <  1:936222/150‑1 (MQ=255)

                                                                                                 gCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGa                                                >  4:506722/1‑150 (MQ=255)

                                                                                                  cATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGAt                                               >  2:480475/1‑150 (MQ=255)

                                                                                                   atatatGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATa                                              <  4:43019/150‑1 (MQ=255)

                                                                                                        tGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAGTCGGTAATATTCATGATaacaac                                         <  3:629500/150‑1 (MQ=255)

                                                                                                         gggACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAAcc                                        >  2:395387/1‑150 (MQ=255)

                                                                                                                 acgcAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGttttt                                                                                                >  2:447224/1‑86 (MQ=255)

                                                                                                                 acgcAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGttttt                                                                                                <  1:447224/86‑1 (MQ=255)

                                                                                                                 acgcAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCa                                                   >  3:18375/1‑131 (MQ=255)

                                                                                                                 acgcAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCa                                                   <  4:18375/131‑1 (MQ=255)

                                                                                                                   gcAAAGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGgcg                              <  1:1611465/150‑1 (MQ=255)

                                                                                                                     aaaGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATATTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCa                            >  3:937927/1‑150 (MQ=255)

                                                                                                                      aaGTGTGCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCaa                           >  4:704675/1‑150 (MQ=255)

                                                                                                                           tgCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCAt                                                  <  2:1099228/122‑1 (MQ=255)

                                                                                                                           tgCTCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCAt                                                  >  1:1099228/1‑122 (MQ=255)

                                                                                                                              tCGCGGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCCAGCCAGGTc                   >  4:727146/1‑150 (MQ=255)

                                                                                                                                gcgGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCg                                                                                                               >  1:1070628/1‑56 (MQ=255)

                                                                                                                                gcgGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAAc                                                                                >  1:736920/1‑87 (MQ=255)

                                                                                                                                gcgGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCgg                 <  1:1263220/150‑1 (MQ=255)

                                                                                                                                gcgGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTAGATAGTTTTTGCGTCAATTCGTTAACGTATTCATACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCgg                 >  3:606383/1‑150 (MQ=255)

                                                                                                                                 cgGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTaa                                                                                 >  1:1080733/1‑85 (MQ=255)

                                                                                                                                 cgGATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTaa                                                                                 <  2:1080733/85‑1 (MQ=255)

                                                                                                                                  ggATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGga               <  4:927242/150‑1 (MQ=255)

                                                                                                                                   gATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGgaa              >  4:66567/1‑150 (MQ=255)

                                                                                                                                   gATTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGgaa              >  4:553223/1‑150 (MQ=255)

                                                                                                                                    aTTTTAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGgaag             >  4:903499/1‑150 (MQ=255)

                                                                                                                                     ttttAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATaacaac                                         >  1:1216818/1‑121 (MQ=255)

                                                                                                                                     ttttAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACCAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATaacaac                                         <  2:1216818/121‑1 (MQ=255)

                                                                                                                                        tAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAAt                                                               <  4:871591/96‑1 (MQ=255)

                                                                                                                                        tAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAAt                                                               >  3:871591/1‑96 (MQ=255)

                                                                                                                                        tAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAAc         <  2:668025/150‑1 (MQ=255)

                                                                                                                                        tAAAGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAAc         >  3:401571/1‑150 (MQ=255)

                                                                                                                                          aaGCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCa       >  4:1000401/1‑150 (MQ=255)

                                                                                                                                            gCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAAc         <  3:186555/146‑1 (MQ=255)

                                                                                                                                            gCCAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAAc         >  4:186555/1‑146 (MQ=255)

                                                                                                                                             ccAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCAtt                                                                                                           >  2:469792/1‑47 (MQ=255)

                                                                                                                                             ccAATCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCg    <  1:1348409/150‑1 (MQ=255)

                                                                                                                                               aaTCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGaa  >  4:476636/1‑150 (MQ=255)

                                                                                                                                               aaTCGTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGaa  >  4:105979/1‑150 (MQ=255)

TCGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAA  >  NC_000913_3_pae_tpiA/1800694‑1800986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`