Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913_3_pae_tpiA	1,978,503	Δ776 bp	100%		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_tpiA	1978503	1979278	776	50 [0]	[0] 51	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 1978502	0 (0.000)	47 (0.960)	34/256	NT	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_pae_tpiA	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

CTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATA  >  NC_000913_3_pae_tpiA/1979132‑1979426                                                                                                                                                    |                                                                                                                                                    gaaatgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgta                                                                                                                                                    <  1:826506‑M2/2‑1 (MQ=255)       ggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTc                                                                                                                                              >  1:814633‑M2/142‑149 (MQ=255)       ggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTc                                                                                                                                              >  1:546923‑M2/142‑149 (MQ=255)            ttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACt                                                                                                                                         >  1:523131‑M2/137‑149 (MQ=255)            ttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattaattaacataaatgtataagcactact                                                                                                                                         >  2:934445‑M2/137‑142 (MQ=255)                gcttatccaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttg                                                                                                                                     <  1:189334‑M2/17‑1 (MQ=255)                acttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttg                                                                                                                                     >  1:900544‑M2/133‑149 (MQ=255)                acttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggccgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACgtttg                                                                                                                                     >  2:685018‑M2/133‑149 (MQ=255)                      ctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgagccagatcacacaaaacactcaattacttaacataaaggTATAGGGGATacttgtgttttcg                                                                                                                               >  1:48536‑M2/127‑147 (MQ=255)                      ctaagatatttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggtgctgcgattttcaataatgcgtgatgcatatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                               <  2:914684‑M2/23‑1 (MQ=255)                       taagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTTGTTTggg                                                                                                                              >  2:711838‑M2/126‑149 (MQ=255)                              tttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTAttt                                                                                                                       >  1:188253‑M2/119‑149 (MQ=255)                                   taaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATc                                                                                                                  >  1:587189‑M2/114‑149 (MQ=255)                                   taaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATc                                                                                                                  >  1:775932‑M2/114‑149 (MQ=255)                                      atcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttaaatacaacgtgaagcagaacacacaagacactcaattacttaacgtaaatgTATAAGGCATACTTTTGTTTTGGGTGTATGtaaatattt                                                                                                               >  1:464399‑M2/111‑145 (MQ=255)                                       tcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtaatgcagatcacacaaaacactcaattacttaacataaatgGATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGtt                                                                                                              >  1:898926‑M2/110‑149 (MQ=255)                                         gacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttcacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaa                                                                                                            >  1:597032‑M2/108‑149 (MQ=255)                                              aactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGt                                                                                                       >  1:471707‑M2/103‑149 (MQ=255)                                               actgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGtt                                                                                                      >  2:599427‑M2/102‑149 (MQ=255)                                                     ctcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgc                                                                                                >  1:726571‑M2/96‑149 (MQ=255)                                                         tcaccacacgcacatacaacggagtggggctgtgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTtcgctact                                                                                            <  2:63785‑M2/58‑2 (MQ=255)                                                                     catacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaa                                                                                >  2:73135‑M2/80‑149 (MQ=255)                                                                      atacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaa                                                                               >  2:904395‑M2/79‑149 (MQ=255)                                                                            cggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaaa                                                                         <  2:788274‑M2/77‑1 (MQ=255)                                                                            cggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaaa                                                                         <  2:775932‑M2/77‑1 (MQ=255)                                                                            aggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaactctcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaaa                                                                         <  2:322986‑M2/77‑1 (MQ=255)                                                                               aggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATATGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAg                                                                      >  1:724263‑M2/70‑149 (MQ=255)                                                                                 gggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGa                                                                    >  2:311339‑M2/68‑149 (MQ=255)                                                                                  ggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaa                                                                   <  2:90534‑M2/83‑1 (MQ=255)                                                                                     gctgcgattttcaataatgcgtgatgcagatcacacagaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaataa                                                                >  1:326672‑M2/64‑149 (MQ=255)                                                                                     gctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTTGCAAGCAAAAATGAAACAGGaataa                                                                >  1:547237‑M2/64‑149 (MQ=255)                                                                                     gctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAACCAAAAATGAAACAGGaaaaa                                                                >  1:546310‑M2/64‑149 (MQ=255)                                                                                       tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGTTGTATTTCAATCTGTTAAAAAGTTTTTCTCTACGCTAGCAAGCAAAAATGAAACAGGCATAATc                                                              >  2:69117‑M2/62‑149 (MQ=255)                                                                                       tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGTTAGCAAGCAAAATTGAAACAGGAATAATc                                                              >  1:160501‑M2/62‑149 (MQ=255)                                                                                       tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATc                                                              >  1:712341‑M2/62‑149 (MQ=255)                                                                                            ttttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAAt                                                         >  1:544164‑M2/57‑149 (MQ=255)                                                                                                   taatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAACATGAAACAGGAATAATAGAAATGGgattt                                                  >  2:127161‑M2/50‑147 (MQ=255)                                                                                                         gtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGAAATAATCGAAATGGGATGTTGCGca                                            >  1:727965‑M2/44‑149 (MQ=255)                                                                                                         gtgatgcagaacacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGca                                            <  2:145076‑M2/106‑1 (MQ=255)                                                                                                           gatgcagatcacacaaaactctcgattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGcaca                                          <  2:756387‑M2/108‑1 (MQ=255)                                                                                                           gatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGcaca                                          <  1:703833‑M2/108‑1 (MQ=255)                                                                                                           gatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGcaca                                          <  1:796071‑M2/108‑1 (MQ=255)                                                                                                                   tcacacaaaaccctcaattacttaatataaatgTATAAGTCATACTGTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAAt                                  <  1:726334‑M2/116‑1 (MQ=255)                                                                                                                        caaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATaacat                             >  2:368650‑M2/29‑147 (MQ=255)                                                                                                                         aaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCa                            >  1:521300‑M2/28‑149 (MQ=255)                                                                                                                            acactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCg                         >  1:316023‑M2/25‑149 (MQ=255)                                                                                                                              actcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTa                       >  2:623979‑M2/23‑149 (MQ=255)                                                                                                                                  aattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGCATGTTGCGCACAGTCAAAATAACTCACCGTAaata                   >  1:639918‑M2/19‑149 (MQ=255)                                                                                                                                                aatgTATACGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGAAAAAATGAAACAGGAATAGTCGAAATGGGATGTTTCGCACAGTCAAAATAAATAACCGTGAATAATCATTTGctatat     >  2:280341‑M2/5‑148 (MQ=255)                                                                                                                                                  tgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTataaat   <  2:188253‑M2/147‑1 (MQ=255)                                                                                                                                                   ttataTGTCATATTTTTGTCTTGTGTTTAGTCCAATATGTTAAATAGTTATTCGCTACACTAGCAAGCAAAAATGAAACAGAAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATTTGCTataaata  <  2:112219/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATA  >  NC_000913_3_pae_tpiA/1979132‑1979426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑