Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 1,933,131 G→A 8.9% P492S (CCG→TCG)  edd ← 6‑phosphogluconate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,933,1310GA 8.9% 111.1 / 3.1 45P492S (CCG→TCG) edd6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (17/24);  new base A (2/2);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAAT  >  NC_000913_3_pae_tpiA/1932993‑1933275
                                                                                                                                          |                                                                                                                                                
cTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGGAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTa                                                                                                                                        <  1:698315/149‑1 (MQ=255)
 ttttTCCAGCAGCCCGCCATCGTAGGGTTCTGGCGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTAt                                                                                                                                       <  2:15991/149‑1 (MQ=255)
 ttttGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTAt                                                                                                                                       <  1:551162/149‑1 (MQ=255)
       cagcagCCCGCCATCGTAGGCTTCTGGTGTTACGTTGATAGCTGACGGCACTTTATCTAAAACGCAGAAGAGTCGTCCATAGGTAACTAACGAAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAGtt                                                                                                                                 >  1:702551/1‑149 (MQ=255)
          cagcCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTg                                                                                                                              <  1:184066/149‑1 (MQ=255)
           agcCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTgg                                                                                                                             <  1:920978/149‑1 (MQ=255)
           agcCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTgg                                                                                                                             <  1:114130/149‑1 (MQ=255)
             cccGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCa                                                                                                                           >  1:447061/1‑149 (MQ=255)
               cGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACATCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCAtt                                                                                                                         <  1:483338/149‑1 (MQ=255)
               cGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCAtt                                                                                                                         >  1:834817/1‑149 (MQ=255)
               cGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCAtt                                                                                                                         >  1:834676/1‑149 (MQ=255)
                 ccATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCAAATAATACACCAAGTGGCGGCATGAGTGTAAGTCATTCTTGCAttaa                                                                                                                       >  2:653908/1‑147 (MQ=255)
                          gCTTCTGGTGGTGGGTGGATGGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAGACACCGGTCCAATAATACATCACGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCttt                                                                                                              <  2:229552/149‑1 (MQ=255)
                             tCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCCATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCATTCGCTTTTgg                                                                                                           >  1:343737/1‑149 (MQ=255)
                             tCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTgg                                                                                                           >  2:354101/1‑149 (MQ=255)
                                    ttgagTGGATAGCTGACGGCCCTTTACCTGAGGCGCCGGAGAGTCTTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAACAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTGTGGCCCCtga                                                                                                    <  2:15431/145‑1 (MQ=255)
                                    tgaCGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCtga                                                                                                    <  2:94462/147‑1 (MQ=255)
                                       cGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCtgatga                                                                                                 <  1:554596/149‑1 (MQ=255)
                                          ggATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATCCACCAAGTGGCGGCATCAGCACATGAAAATCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACgg                                                                                              >  1:264994/1‑149 (MQ=255)
                                          ggATAGCTGACGGCACTTGACCTGAAGCGCCGGAGAGTCGTCCATCGTTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTGCGCTTTTGGCCCCTGATGACgg                                                                                              <  2:375065/149‑1 (MQ=255)
                                            aTAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGac                                                                                            >  1:699791/1‑149 (MQ=255)
                                              aGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGacaa                                                                                          <  1:861092/149‑1 (MQ=255)
                                                cTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCt                                                                                                      <  2:224684/135‑1 (MQ=255)
                                                cTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCt                                                                                                      >  1:224684/1‑135 (MQ=255)
                                                 tGACGGCCCTTTACCTGAAGCGCCGGAGAGGCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGAcaacaa                                                                                       <  1:182505/149‑1 (MQ=255)
                                                 tGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGACATGAGTTTATGTAATTCTGGCATTCCGtt                                                                                                                    >  1:185169/1‑120 (MQ=255)
                                                 tGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGACATGAGTTTATGTAATTCTGGCATTCCGtt                                                                                                                    >  1:185364/1‑120 (MQ=255)
                                                 tGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGACATGAGTTTATGTAATTCTGGCATTCCGtt                                                                                                                    <  2:185169/120‑1 (MQ=255)
                                                 tGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGACATGAGTTTATGTAATTCTGGCATTCCGtt                                                                                                                    <  2:185364/120‑1 (MQ=255)
                                                    cGGCACTTTACCTGAAGCGCCGGAGACTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCCGCATGAGTTTATGTAATTCTGGCATCCCGTACGCGGTTGGCCCCTGATGACGGACAACAACGa                                                                                    <  2:299010/149‑1 (MQ=255)
                                                          tttACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATCCACCAAATGGCGGCATGAGTTTATGTAATTATGGCATTCCGTTCGCTTTTGGCCCCTGATTACGGACAACAACGACACAAt                                                                              >  1:924762/1‑149 (MQ=255)
                                                              ccTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATcgcg                                                                          <  1:210644/149‑1 (MQ=255)
                                                               cTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCgg                                                                         <  2:716536/149‑1 (MQ=255)
                                                                tGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGNCGGACAACAACGACCCAATCGCGGt                                                                        >  1:621111/1‑149 (MQ=255)
                                                                                     aTCTGTAACGAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATGCTGGCATTCCGTTCGCTTTTCGCCCCTCATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAgg                                                   <  2:427238/149‑1 (MQ=255)
                                                                                       cgtgaaccaacGCAATTTTGAAACACCGGTCCAATAATACACCCAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGcc                                                 <  2:176432/145‑1 (MQ=255)
                                                                                           aactaacGCAATTTTGAAACACCGGTCCAATAATACACCAAGGGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCa                                             <  1:349838/149‑1 (MQ=255)
                                                                                              taacGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGTCGGCATGAGTTTATGTAATTCTGACATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTGCAGCAAACCCGCTTCAAAGCCCAGCATaa                                          >  2:651542/1‑149 (MQ=255)
                                                                                               aacGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAAc                                         <  1:813821/149‑1 (MQ=255)
                                                                                                      ttttGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATAGCGGTCAAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGc                                  >  1:721825/1‑149 (MQ=255)
                                                                                                               acCGGTCCAATAATACACCAAGTGGCGGCATGAGTCTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCa                         >  2:630960/1‑149 (MQ=255)
                                                                                                                            tccacCAAGTGGCGGCATGAGTTTATGTAATTCTGGCAGTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTg            <  1:292379/147‑1 (MQ=255)
                                                                                                                             acacCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTgg           >  1:476926/1‑149 (MQ=255)
                                                                                                                               acCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCCTTCAAAAACAACCGCTGgcg         >  2:44758/1‑149 (MQ=255)
                                                                                                                                  aaGTGCCGGCATTAGTTTATGTAATTCTGCCATTCCGTTCGCTTGTGGCCCTCGATGACCGGCAACAACGACACAATCGCGGTCCAGAAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCtt      <  2:343737/149‑1 (MQ=255)
                                                                                                                                      ggcggcATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTAGCGCTTCAAt  >  2:703854/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                
CTTTTGCCAGCAGCCCGCCATCGTAGGCTTCTGGTGTTACGTGGATAGCTGACGGCACTTTACCTGAAGCGCCGGAGAGTCGTCCATCGGTAACTAACGCAATTTTGAAACACCGGTCCAATAATACACCAAGTGGCGGCATGAGTTTATGTAATTCTGGCATTCCGTTCGCTTTTGGCCCCTGATGACGGACAACAACGACACAATCGCGGTCCAGCAAACCCGCTTCAAAGGCCGGCATAACGTCATGCTGGCTTTCAAAAACAACCGCTGGCGCTTCAAT  >  NC_000913_3_pae_tpiA/1932993‑1933275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑