Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 2,106,741 A→T 100% S162S (TCT→TCA)  wbbH ← O‑antigen polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,106,7410AT100.0% 200.2 / ‑6.7 62S162S (TCT→TCA) wbbHO‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (28/33);  minor base G (0/1);  total (28/34)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTC  >  NC_000913_3_pae_tpiA/2106599‑2106885
                                                                                                                                              |                                                                                                                                                
tATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCaa                                                                                                                                            >  1:304672/1‑149 (MQ=255)
 aTTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAACTGCAAAGAGGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCaaa                                                                                                                                           <  1:601725/149‑1 (MQ=255)
 aTTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACAAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTAACTTTGTATTAGTAAATTTTTTTGACTaat                                                                                                                                           >  2:463380/1‑148 (MQ=255)
   tAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTGACCTTTGTGTTAGTAAATTTTTTTGACCaaata                                                                                                                                         >  1:807420/1‑149 (MQ=255)
     acacCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCaaataaa                                                                                                                                       >  1:870349/1‑149 (MQ=255)
        tcTACGATTAATCCATAAGAGATGATAACCATAAAGACAAGTTGCTTACCAGTATTCAGGATAATTGCAAAGATGAGTACAATAAAAATAAATATATCAGATGTTTTACGTACCTTTGTATTAGTAAATTTTTTTGACCAAataaataa                                                                                                                                    <  1:186065/148‑1 (MQ=255)
          tACGATGAATGCATAATAGAAGATAACCATACAGACAATTTGCTTACCAGTTATCGTAAGAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAg                                                                                                                                  <  1:220722/149‑1 (MQ=255)
               tGAAGGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCaaaa                                                                                                                             <  1:5284/149‑1 (MQ=255)
                gAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAag                                                                                                                            >  1:324346/1‑149 (MQ=255)
                   tGCATAAGAGATGATAACCATAAAGACAAATTGCTTATCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTGCCTTTGTATTAATAAATTTTTTTGACCAAATAAATAAAGCAAAagtag                                                                                                                         >  1:614579/1‑149 (MQ=255)
                     cATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGtt                                                                                                                       >  2:467355/1‑149 (MQ=255)
                           agaTGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGAAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGAAAAAGTAGTTAGAATg                                                                                                                 >  2:807379/1‑149 (MQ=255)
                                    ccATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGCTCCAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGc                                                                                                        >  1:415213/1‑149 (MQ=255)
                                      aTAAAGACAATGTGCTTACCAGTATTTAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCAt                                                                                                      <  2:363465/149‑1 (MQ=255)
                                       tAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATATAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATg                                                                                                     >  2:245336/1‑149 (MQ=255)
                                              aaTTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTg                                                                                              >  1:841921/1‑149 (MQ=255)
                                                     ttACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGGAGTTAGAAAGATTGGCTGCATCTATGCTGAGAAAtt                                                                                       >  1:159565/1‑149 (MQ=255)
                                                         cAGTATTCAGTATAATTGCAAAGATGAATACAATAAACACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGATCAAATAAATAAAGCTTAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCtt                                                                                   <  2:4632/149‑1 (MQ=255)
                                                           gTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGa                                                                                 <  2:23625/149‑1 (MQ=255)
                                                           gTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGa                                                                                 <  2:587587/149‑1 (MQ=255)
                                                            tATTCAGTATAATTGCAAAGCTGACTACAATAATAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTCGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTGGGCATGATTGGCTGCGTGTATGCTGTGAAATTTCTTGAt                                                                                <  1:810243/149‑1 (MQ=255)
                                                              ttCAGTAGAATGGCAAATATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAtgt                                                                              <  1:258779/149‑1 (MQ=255)
                                                               tCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAtgtg                                                                             >  1:443803/1‑149 (MQ=255)
                                                                  gTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAGTAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCt                                                                          >  1:515971/1‑149 (MQ=255)
                                                                   tataATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCtt                                                                         >  2:863108/1‑149 (MQ=255)
                                                                   tataATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAATAGGTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCtt                                                                         <  1:426026/149‑1 (MQ=255)
                                                                    ataATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTc                                                                        <  2:565719/149‑1 (MQ=255)
                                                                    ataATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTc                                                                        <  1:458223/149‑1 (MQ=255)
                                                                    ataATTGCAAAGATGAATAAAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTc                                                                        <  1:458064/149‑1 (MQ=255)
                                                                     taATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACTAAATAAATAAAGAAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCa                                                                       >  1:917154/1‑149 (MQ=255)
                                                                     taATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCa                                                                       >  2:94775/1‑149 (MQ=255)
                                                                     taATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCa                                                                       >  2:629667/1‑149 (MQ=255)
                                                                     taATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCa                                                                       >  2:630047/1‑149 (MQ=255)
                                                                     taATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCa                                                                       >  1:803424/1‑149 (MQ=255)
                                                                      aaTTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCaa                                                                      <  1:936085/149‑1 (MQ=255)
                                                                      aaTTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCaa                                                                      <  2:790781/149‑1 (MQ=255)
                                                                         cgccaaGATGAATGCGATAAAATCAACGAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACAt                                                                   >  2:907339/5‑149 (MQ=255)
                                                                           cAAAGATGAATACAATAAAAACAAGTAAAGTAAATTGTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAACAAAGCCAAAGTAGTTAGCATCATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCa                                                                 <  2:690068/149‑1 (MQ=255)
                                                                           cAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTCACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAAAAATTACTTGATGTGTCTTCAACATCa                                                                 >  1:370719/1‑149 (MQ=255)
                                                                           cAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTAGTAGTAAATTTTTTTGACCAAAGAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCa                                                                 <  1:398880/149‑1 (MQ=255)
                                                                                  gAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCAtctc                                                          >  1:203073/1‑149 (MQ=255)
                                                                                          tAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCaaa                                                  <  2:870476/149‑1 (MQ=255)
                                                                                           aaaaaCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAATAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAAt                                                 <  2:614579/149‑1 (MQ=255)
                                                                                              aaCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCa                                              <  1:670047/149‑1 (MQ=255)
                                                                                              aaCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAACGCTTAGCTGCCTGTATGCTGAGAAATTTCTTGCTGTATCTCAAACACCAGCATCTCTTATCAAATTCa                                              <  2:689773/149‑1 (MQ=255)
                                                                                               ataagtaaagtaAATGTTTTACTTCCCTTTCTATTAGTAAAGTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCat                                             <  1:500806/147‑1 (MQ=255)
                                                                                                ccagtatagtaaATGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCata                                            <  1:74844/147‑1 (MQ=255)
                                                                                                    taaagtaaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACACCAGCATCTCTTATCAATTTCatgttgt                                        >  2:517678/1‑144 (MQ=255)
                                                                                                       attacaTGTTTTACTTTGCTTTGTAGTAGTAAATTTTTTTGACCTAATAAATATAGCAAAAGTAGTTAGAATGATTGTCTACATGTATTTTTAGAGATTTCTTGATGTGTCTTCAATATTAGTATTTCTTATCAAATTCATATAGCtaa                                     <  2:399403/144‑1 (MQ=255)
                                                                                                       agtaaaTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaa                                     <  1:711066/149‑1 (MQ=255)
                                                                                                           aaTGATTTTCTTACCTTCTTATTAGTAAATTTTTTTGACAAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTTCATGTATGCTGAGAAATTTTTTTGTGTGTCTTCAACATTAGCATCTCTTATCAAATTCATATAGCtaagtaa                                 <  2:444753/149‑1 (MQ=255)
                                                                                                            aTGTTTTACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCtaagtaag                                <  1:78401/149‑1 (MQ=255)
                                                                                                               ttttACTTACCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTa                             <  1:21750/149‑1 (MQ=255)
                                                                                                               ttttACTTACCTTTGTATTAGTAAATTTTTTTGACAAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTa                             >  1:784399/1‑149 (MQ=255)
                                                                                                                       acttttGTATTAGTAAATTTTTTGAACAAAATAAATAAAGCAAAAGTAGTTAGAATGATTGACTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGaa                     <  2:145741/146‑1 (MQ=255)
                                                                                                                       acCTTTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGaa                     <  2:749145/149‑1 (MQ=255)
                                                                                                                       acCTTTGGATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGaa                     <  1:793447/149‑1 (MQ=255)
                                                                                                                       acCTGTGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGCTTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGaa                     <  1:609224/149‑1 (MQ=255)
                                                                                                                          tttGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTg                  >  1:105604/1‑149 (MQ=255)
                                                                                                                          tttGTATTAGTAAATTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTg                  <  2:832156/149‑1 (MQ=255)
                                                                                                                                     aaaTTTTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTa       >  2:591023/1‑149 (MQ=255)
                                                                                                                                          ttttTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTc  >  1:595496/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                
TATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTTTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTCTTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAACCTC  >  NC_000913_3_pae_tpiA/2106599‑2106885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑