Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 2,172,505 A→T 6.2% V123V (GTT→GTA)  gatD ← galactitol‑1‑phosphate dehydrogenase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,172,5050AT 6.2% 162.4 / 2.2 61V123V (GTT→GTA) gatDgalactitol‑1‑phosphate dehydrogenase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (21/34);  new base T (2/2);  total (25/36)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

CAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGCACACAGGCAACCGCATCGCCAGGGTGTAAAT  >  NC_000913_3_pae_tpiA/2172357‑2172653
                                                                                                                                                    |                                                                                                                                                    
cAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTa                                                                                                                                                      >  1:333704/1‑149 (MQ=255)
     gTTCCGGCACAAATAATAATATCGTTTTTATTCTCACGACCTTGCGCTAAATGAAAAGCATGTAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTAACTAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGAc                                                                                                                                                 <  2:247075/149‑1 (MQ=255)
        ccGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAat                                                                                                                                              <  2:905081/149‑1 (MQ=255)
             aCCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCAGTGGGTAAAGCAAAGACATTTTTTCGCTTAACGACAATATAtt                                                                                                                                         >  2:466719/1‑149 (MQ=255)
               cAATAATAATAACTGTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCa                                                                                                                                       <  2:592899/149‑1 (MQ=255)
               cAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCa                                                                                                                                       <  1:622626/149‑1 (MQ=255)
               cAATAATAATAACGTTTTTATTCTCACAACCATACGCTAAATAAAAACCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCa                                                                                                                                       <  2:748963/149‑1 (MQ=255)
                aataataatAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGCCAATATCTTCAg                                                                                                                                      >  1:352443/1‑149 (MQ=255)
                aataataatAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAg                                                                                                                                      <  1:831087/149‑1 (MQ=255)
                                      tcACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGa                                                                                                                >  2:143672/1‑149 (MQ=255)
                                          aaCCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCa                                                                                                            >  2:577179/1‑149 (MQ=255)
                                                gcgcCAAATGAAAAGCATGCATACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATaaaa                                                                                                      <  1:365865/149‑1 (MQ=255)
                                                   cTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCa                                                                                                   >  1:759992/1‑149 (MQ=255)
                                                          aaaaGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTg                                                                                            <  1:360115/149‑1 (MQ=255)
                                                           aacgCATGCAGGCCAACGGTAATCGGCTCAATAAACGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTgc                                                                                           <  1:115294/146‑1 (MQ=255)
                                                            aaGCATGCAGACCAACGATAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTgcg                                                                                          <  1:698609/149‑1 (MQ=255)
                                                                 tGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCAcgccgc                                                                                                                  >  2:38583/1‑120 (MQ=255)
                                                                 tGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCAcgccgc                                                                                                                  <  1:38583/120‑1 (MQ=255)
                                                                 tGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTg                                                                                     >  2:933163/1‑149 (MQ=255)
                                                                 tGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTg                                                                                     >  1:143112/1‑149 (MQ=255)
                                                                          aCGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGCCATTTTCTCGCTTAACGACAATATATTCAGCAGATCCACCATCATGCCGCGAGCCAATAAAGTCATATTTTGCGCAATGGGAATaaaa                                                                            <  1:538695/149‑1 (MQ=255)
                                                                             gTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAAccc                                                                         <  2:95758/149‑1 (MQ=255)
                                                                                aTCGGCTCAATAAAAGCCCCATCCTCAATAGGAATATCCGTGGGTAGAGCAAAGACATTTTTTCACTTAACGACAATATATTCAGCAAATCCACCATCACGCAGCGAGCCTATAAAATCATATATTGCGCACTGGGAATACTACCGttt                                                                      >  2:382114/1‑149 (MQ=255)
                                                                                aTCCGCTCAATAAAGGCCCCATCCTCCAGAGGCATATCCGTAGGTAGATCAAAGACATTTTTTCGCTAAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCttt                                                                      <  2:214655/149‑1 (MQ=255)
                                                                                 ttggCTCAATAAAAGCCCCATCCCGAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTc                                                                     <  1:496245/147‑1 (MQ=255)
                                                                                 tCGGCTGAATAAAAGCCCCATCTTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTc                                                                     <  2:534215/149‑1 (MQ=255)
                                                                                 tCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCCCGAGCCAATAAAATCACATTTCGCGCACCAGAAATAAAACCCTTTc                                                                     >  1:90087/1‑149 (MQ=255)
                                                                                    gCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGa                                                                  >  1:843802/1‑149 (MQ=255)
                                                                                    gCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGa                                                                  >  1:386744/1‑149 (MQ=255)
                                                                                        aaTAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACAct                                                              >  2:123908/1‑149 (MQ=255)
                                                                                         aTAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCAAGAACCAAGAAACTCATATTTTGCACCCTTTGAATAAAACCCTTTCAGACActc                                                             >  1:824102/1‑149 (MQ=255)
                                                                                          tAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACActct                                                            <  2:69981/149‑1 (MQ=255)
                                                                                           acaaGCCCCATCCTCAATACGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAATGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCCATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTg                                                           <  1:120768/147‑1 (MQ=255)
                                                                                                   cATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTCCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTa                                                   >  2:565215/1‑149 (MQ=255)
                                                                                                     tCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGACGCGAGCCAATAAAATCATATTTTGCGCACTGGGATTAAGATACTTTCACATAATCTGGACAAGTact                                                 >  2:877588/1‑147 (MQ=255)
                                                                                                      ccTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTaaaa                                                <  1:697884/149‑1 (MQ=255)
                                                                                                       cTCAATCGTCATATCCGTGGGTAGATCAAAGACATTTTTTCGCTTAGCGATAATATATGCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGCGTCGGCAAGGCGTGCAGACACTCTTGACAAGTAAAAc                                               <  2:445869/149‑1 (MQ=255)
                                                                                                        tCAATAGGCATATCCGTGGGTAGAGCAATGACCTTTTTTCTGTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACa                                              <  1:636153/149‑1 (MQ=255)
                                                                                                          aaTAGGCCTATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAgg                                            <  1:394079/149‑1 (MQ=255)
                                                                                                          aaTAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAATGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTAAGACAATCTGGACAAATaaaaaaag                                            >  2:713465/1‑145 (MQ=255)
                                                                                                          aaTAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAgg                                            >  1:34144/1‑149 (MQ=255)
                                                                                                               gCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGtaat                                       <  2:590053/149‑1 (MQ=255)
                                                                                                                      cGTGGGAAGAGGTAAGACATTTTTCCGCGTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGca                                <  2:334047/149‑1 (MQ=255)
                                                                                                                        tGGGTAGAGCAAAGACATTTTTTCGTTTTATTACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGcaca                              >  2:342887/1‑149 (MQ=255)
                                                                                                                        tGGGTAGAGCAAAGACATTTTTTCGCTTTACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGcaca                              >  2:342903/1‑149 (MQ=255)
                                                                                                                        tGGGTAGAGCAAAGACATTTTTTCGCTTTACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGcaca                              <  1:342903/149‑1 (MQ=255)
                                                                                                                        tGGGTAGAGCAAAGACATTTTTTCGCTTTACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGcaca                              <  1:342887/149‑1 (MQ=255)
                                                                                                                        tGGGTAGAGCAAAGACATTTTTTCGCTTGACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTACAACAGGGTAATAACGGcaca                              >  2:86171/1‑149 (MQ=255)
                                                                                                                         gggTAGAGCAAAGACATTTTTTCGCTTCACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGcacac                             >  2:66224/1‑149 (MQ=255)
                                                                                                                           gTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATATAACCCCTTCAGCAATTCTCGACCAGTAGAACAGTTTAATTACGGctcacgg                           <  2:326502/149‑3 (MQ=255)
                                                                                                                                tcAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGTCAATAAAATCATATTTTGCGTACTGGGAATAAAACCCTTTCAGACACTCTAGACAAGTAAAACAGTGTAATAACGGCACACAGGCAAc                      <  2:115196/148‑1 (MQ=255)
                                                                                                                                    aGACATTTCGTCGATCAACGACAATATAGTCAGCAAATCCACGATCACGCCGCTATCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGCACACAGGCAACCGCa                  <  2:425397/149‑1 (MQ=255)
                                                                                                                                     gACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGCACACAGGCAACCGCAt                 >  1:97063/1‑149 (MQ=255)
                                                                                                                                      aCATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTAGACAAATAAAACAAAGTAATAACGGCACACAGACAACCGCATc                <  2:208255/149‑1 (MQ=255)
                                                                                                                                        aTTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCGAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGCACACAGGCAACCGCATCGc              >  1:182289/1‑149 (MQ=255)
                                                                                                                                        aTTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGcac                               <  2:792873/132‑1 (MQ=255)
                                                                                                                                        aTTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGcac                               >  1:792873/1‑132 (MQ=255)
                                                                                                                                           ttttCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGCACACAGGCAACCGCATCGCCAg           <  2:759992/149‑1 (MQ=255)
                                                                                                                                            tttCGCTTAACGACAATTTATTCAGCAAATCCACCATCACGCCGCTAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGCACACAGGCAACCGCATCGCCAgg          <  2:790484/149‑1 (MQ=255)
                                                                                                                                             ttcctttAACCACAATATATTCACCAAATCCACCATCCCGCCGCGAGCCAATAAAATCATATTTTGCTCACTGGTAATAAAACCCTTTCAGACACGCTGGACAAGTAAAACAGGGTAATAACGGCACACAGGCAACCGCATCGCCAggg         <  1:197374/144‑1 (MQ=255)
                                                                                                                                              tCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGCACACAGGCAACCGCATCGCCAGGgt        <  2:668879/149‑1 (MQ=255)
                                                                                                                                                  ttACCGACAATATATGCAGCAAATCCAGCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGCACACAGGCAACCGCATCGCCAGGGTGTaa    <  2:35025/149‑1 (MQ=255)
                                                                                                                                                    aaCGACAATATACTCAGCAAATCCACCATCACGCCGCGAGCCAATACAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAACCAGGGTAATAACGGCACACAGGCAACCGCATCGCCAGGGTGTAAat  <  1:467049/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGACATTTTTTCGCTTAACGACAATATATTCAGCAAATCCACCATCACGCCGCGAGCCAATAAAATCATATTTTGCGCACTGGGAATAAAACCCTTTCAGACACTCTGGACAAGTAAAACAGGGTAATAACGGCACACAGGCAACCGCATCGCCAGGGTGTAAAT  >  NC_000913_3_pae_tpiA/2172357‑2172653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑