Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 3,440,923 C→T 49.7% G36D (GGC→GAC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,440,9230CT49.7% ‑3.5 / 82.4 64G36D (GGC→GAC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (16/16);  new base T (15/17);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-02

CAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAA  >  NC_000913_3_pae_tpiA/3440775‑3441068
                                                                                                                                                    |                                                                                                                                                 
cAGGTTGAGGAGGATTTCCAGGATATCTTCCTGAACCCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGc                                                                                                                                                   <  2:774579/149‑1 (MQ=255)
          aGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACg                                                                                                                                         >  1:852080/1‑149 (MQ=255)
                 ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTaaa                                                                                                                                  <  1:753291/149‑1 (MQ=255)
                   aGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAgg                                                                                                                                >  1:506702/1‑149 (MQ=255)
                         tCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTCAAGGCTCAAg                                                                                                                          >  2:361687/1‑149 (MQ=255)
                         tCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCCCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCACTAGAGGCTCAAg                                                                                                                          >  2:259808/1‑149 (MQ=255)
                         tCTTCCTAAACGCCTTCTTTTGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCTATGAGAGCATAATACGGCGCAGTGCGTTACCCATAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAg                                                                                                                          <  2:648413/149‑1 (MQ=255)
                          cTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTGCCCAGAGTATGGTCCTAGCCACTCTCTAAAGGCTCAAgg                                                                                                                         <  2:631489/149‑1 (MQ=255)
                          cTTCCTGAACGCCTTCTTTCGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                         <  1:840447/149‑1 (MQ=255)
                               tGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCAc                                                                                                                    >  1:531383/1‑149 (MQ=255)
                                 aaCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCt                                                                                                                  >  1:536307/1‑149 (MQ=255)
                                      cttcttTGGTGCTGTACTCATGTAGTCCTCTAGGAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcg                                                                                                             >  2:446372/1‑149 (MQ=255)
                                       ttcttTGGTGCTGTACTCATGTAGTACCCCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGTGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGACTCACGGGTCACCTTGgcgt                                                                                                            >  2:325930/1‑149 (MQ=255)
                                       ttcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgt                                                                                                            >  2:823075/1‑149 (MQ=255)
                                       ttcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTAACCTTGgcgt                                                                                                            >  2:673021/1‑149 (MQ=255)
                                                      cTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACtt                                                                                             <  1:703447/149‑1 (MQ=255)
                                                       tCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTg                                                                                            >  2:701105/1‑149 (MQ=255)
                                                       tCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTg                                                                                            >  2:707930/1‑149 (MQ=255)
                                                         acttagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                          <  1:116165/146‑1 (MQ=255)
                                                               taCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                    >  1:528539/1‑149 (MQ=255)
                                                                   ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCCCCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAc                                                                                <  2:298928/149‑1 (MQ=255)
                                                                    cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGCTAGAAGTAGAATAGGGCGCAATTCGTGACCCACATTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCCAACGAACTTGCACGATATCAAcc                                                                               >  2:202435/1‑149 (MQ=255)
                                                                    cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGCGTATGGTCAAAGCCACGCCCCAAAGGCTCAAGGGTCACCTCGGCGTGCGTCGCACCCCCTTGCTAGATACCacca                                                                               >  2:493737/1‑146 (MQ=255)
                                                                     aTCAATCTCAAGCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACAGCGCGGCACGGTGCGCGGGGTATGGCCAAAGCCACGCTCTAAAGGCTCGCTGGTCGCGAGGGCGTGCGTCGAGCTCACGTACGCGATATCAACCa                                                                              >  1:779586/1‑149 (MQ=255)
                                                                     aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATCTCAACCa                                                                              >  2:733803/1‑149 (MQ=255)
                                                                     aTCAATCTCAACCTCGGTCACCGCCCAACCCGGCATCCATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCCCTAAAGGCTCACGGGTCACCTTCGCCTGCGTCGAACTCACTTCCTCGATATCAACCa                                                                              >  2:84187/1‑149 (MQ=255)
                                                                     aTCAATCTCAACCTCGGGCACCGCGCACCCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                              <  2:172789/149‑1 (MQ=255)
                                                                            tcAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCgg                                                                       <  2:921966/149‑1 (MQ=255)
                                                                              aaCCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGtt                                                                     <  1:916095/149‑1 (MQ=255)
                                                                              aaCCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGtt                                                                     <  1:916221/149‑1 (MQ=255)
                                                                                 cTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCggactta                                                                  >  1:730008/1‑144 (MQ=255)
                                                                                         ccGCGCAGCCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                          <  1:79581/149‑1 (MQ=255)
                                                                                         ccGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                          <  1:127165/149‑1 (MQ=255)
                                                                                          cgcgCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTg                                                         >  2:378884/1‑149 (MQ=255)
                                                                                            cgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTc                                                       <  1:470156/149‑1 (MQ=255)
                                                                                              cAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGGTTTAGAAACTCTGTcac                                                     >  2:56860/1‑149 (MQ=255)
                                                                                                   cGGCATCGATGAGAGCAGAAGACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAc                                                <  1:899457/149‑1 (MQ=255)
                                                                                                    ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTAGAACTCACTAGCTCGATATCAACCAGGCGCGGTTTTAGAAACCCTGTCACAGAAcc                                               >  2:200639/1‑149 (MQ=255)
                                                                                                    ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAcc                                               <  1:428137/149‑1 (MQ=255)
                                                                                                      cATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCt                                             <  1:378884/149‑1 (MQ=255)
                                                                                                       aTCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTg                                            <  1:195261/149‑1 (MQ=255)
                                                                                                         cGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCa                                          <  2:852080/149‑1 (MQ=255)
                                                                                                         cGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCa                                          <  1:12697/149‑1 (MQ=255)
                                                                                                            tGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAATGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtg                                       <  2:717861/149‑1 (MQ=255)
                                                                                                              agagCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtgtg                                     <  1:387633/149‑1 (MQ=255)
                                                                                                               gagCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGAATTgtgt                                    >  2:383705/1‑149 (MQ=255)
                                                                                                                agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                   >  1:299104/1‑149 (MQ=255)
                                                                                                                  cAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGTTCTGGTGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                 >  1:510189/1‑149 (MQ=255)
                                                                                                                         cGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGTTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTg                          <  1:796255/149‑1 (MQ=255)
                                                                                                                          gtggcAGTGCGGTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACCTACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTgg                         <  2:105981/146‑1 (MQ=255)
                                                                                                                          ggaccAGGGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTACAGGCTCAAGGGTCACCTTGGCGGGCGTCGAACTCACTTGCATAATTTTAACGAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTgg                         <  1:135235/145‑1 (MQ=255)
                                                                                                                           gcgcAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGt                        <  1:446372/149‑1 (MQ=255)
                                                                                                                            cgtcttGCGTTACCTATAGTGTGGTCAAAGCCACGCTCTAACGCCTCAAGGGTCACCTTTGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGACCCCTGCATTGTGTCCTCTCTTTGGTa                       <  2:137860/144‑1 (MQ=255)
                                                                                                                            cgcAGGGCGTGCCCCGGAGTATGGCCAAAGCCACGCTCGAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTa                       <  1:287807/149‑1 (MQ=255)
                                                                                                                                gTGCGTTACCCAGAGTATGGTCCAAGCCATACTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTaa                   >  1:483403/1‑149 (MQ=255)
                                                                                                                                      tACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCCAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTa             <  1:43339/149‑1 (MQ=255)
                                                                                                                                        cccAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt           <  2:416237/149‑1 (MQ=255)
                                                                                                                                         ccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCCTTGTGTCCTCTCTTTGGTACTAAGCTTTACtt          >  1:526393/1‑149 (MQ=255)
                                                                                                                                         ccAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtt          >  1:266039/1‑149 (MQ=255)
                                                                                                                                          cAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCTGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTg         <  1:779062/149‑1 (MQ=255)
                                                                                                                                           agagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTGGCTCGATATCAACCAGGCGCGGGTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCGTTACTTgg        >  1:786528/1‑149 (MQ=255)
                                                                                                                                            gagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa       <  1:856100/149‑1 (MQ=255)
                                                                                                                                               tATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCGCTTGCTCGATATCAACCAGACGCGGATTATGGCACGCCGTCACAGAACCCTGCATCGTGTCCTCTCTTTAGAACTAAGCATTACTTGGAGTa    >  1:221899/1‑149 (MQ=255)
                                                                                                                                               tATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGatca    >  2:531260/1‑146 (MQ=255)
                                                                                                                                                 tGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTaaa  <  2:218493/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CAGGTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAA  >  NC_000913_3_pae_tpiA/3440775‑3441068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑