Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 3,591,716 A→G 7.6% W204R (TGG→CGG)  ugpB ← sn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,591,7160AG 7.6% 147.2 / 3.9 53W204R (TGG→CGG) ugpBsn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (30/19);  new base G (2/2);  total (32/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01

TTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCATCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAG  >  NC_000913_3_pae_tpiA/3591571‑3591864
                                                                                                                                                 |                                                                                                                                                    
ttACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCATCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAAATCCAGGAGTGCTGCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGgc                                                                                                                                                   >  1:918345/1‑149 (MQ=255)
  gcgactcacGTAGCTGAAGTCGCCCTTCTTGTTCATCTCCTCGAGCATGGCTATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGgcgc                                                                                                                                                 <  1:345321/142‑1 (MQ=255)
  aCGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCATCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGgcgc                                                                                                                                                 >  1:523384/1‑149 (MQ=255)
      ccgacGTAGCTGAAGTCGCCCTTCTTGTTCATCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTaaa                                                                                                                                             <  1:386302/149‑1 (MQ=255)
         gtgTAGCGGAAGTCCCCCATCTTGTTCTTCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGGCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGtt                                                                                                                                          <  2:765267/147‑1 (MQ=255)
            tagtcgcaGTCGCCCGTTGTGTTCGTCTCCTCGAGCATGGCGCTGTGCTTCCTCTGCTCCGGCTTATTGGACTCCAGCACCGCGTCCGGGCCGTCAAAGCTGTTGTTTTTGCTGGCAAACGGCGGACCGTTCCAGGCGCTAAAGTTTTc                                                                                                                                       <  2:541587/142‑1 (MQ=255)
            tAGTTGAAGTCGCCCTTCTTGTTCATTTCCTCGGGCATGGCGATGTGTGTCACCTGCTCCGGCTTATTGAACTCCAGTACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTc                                                                                                                                       <  2:214742/149‑1 (MQ=255)
            tAGCTGAAGTCGCCCTTCTTGTTCATCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTc                                                                                                                                       <  1:584770/149‑1 (MQ=255)
                     tCGCCCTTCTTGTTCATCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTTGAt                                                                                                                              >  2:432789/1‑149 (MQ=255)
                                  tCATCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAg                                                                                                                 >  1:304732/1‑149 (MQ=255)
                                     tctcCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGCCCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCg                                                                                                              >  1:245721/1‑149 (MQ=255)
                                     tctcCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCg                                                                                                              >  1:365883/1‑149 (MQ=255)
                                       tcctcGAGCATGGCGATGTGTTTCACCTGCTGCGGCTAAGCTAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATGCAGCCCTGCCGGCCGCt                                                                                                            >  2:858895/1‑149 (MQ=255)
                                                aTGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTTCCAGCCGCTGGCGTAGcc                                                                                                   >  1:112730/1‑149 (MQ=255)
                                                 tGGCGATGTGTTTCACCTGCTCGGGCTTATGGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCg                                                                                                  >  1:484770/1‑149 (MQ=255)
                                                 tGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCAAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGCCGTAGCCg                                                                                                  >  2:235034/1‑149 (MQ=255)
                                                    cGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAATGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGACAGACGCTGACGTAGCCTCAc                                                                                               >  1:592874/1‑149 (MQ=255)
                                                               aCCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCCTAGCCGCACTTCATGCCGGa                                                                                    >  1:275140/1‑149 (MQ=255)
                                                                 cTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAGACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCCGCCGCTGGCGTGGCCGCACTTCATGCCgcggg                                                                                  >  2:873294/1‑145 (MQ=255)
                                                                 cTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCAAGTTGGATCCAGCCCTGCCAGCCGCAGCCGTACCCGCACTTCAAGCCCGAgg                                                                                  >  2:313957/1‑149 (MQ=255)
                                                                       cGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCa                                                                            <  2:604991/149‑1 (MQ=255)
                                                                       cGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCa                                                                            <  2:149786/149‑1 (MQ=255)
                                                                        ggCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAg                                                                           >  2:921492/1‑149 (MQ=255)
                                                                             aTTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCttt                                                                              >  1:739373/1‑141 (MQ=255)
                                                                             aTTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCttt                                                                              <  2:739373/141‑1 (MQ=255)
                                                                               tGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCATTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGACGGAGGCTTTCAGTTTcgcg                                                                    >  1:683488/1‑149 (MQ=255)
                                                                                  aCTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCa                                                                 <  1:696811/149‑1 (MQ=255)
                                                                                  aCTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCACTGCCAGCCGCTGGAGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGcc                                                                 >  2:268655/1‑148 (MQ=255)
                                                                                    tCCAGCACCGCGTCCGTGCCGTGAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCCGCAGCTGGCGTAGCCGCCAATAATGCCGTAGGTTTCTAGTTCCGCGGcttt                                                               <  2:690167/149‑4 (MQ=255)
                                                                                    tCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCGGTTTCGCGGCATa                                                               >  2:283056/1‑149 (MQ=255)
                                                                                     ccAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAg                                                              >  1:293652/1‑149 (MQ=255)
                                                                                      cAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGACCCAGCCTTGCATAACGCGGGTGTAGCCGCACTTCATGCCGTAGGCTTTCAGTTTCGCGGCATAGt                                                             >  1:27219/1‑149 (MQ=255)
                                                                                       aGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGCTCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGTCGGCTTTCAGTTTCGAGGCATAGTc                                                            >  1:304429/1‑149 (MQ=255)
                                                                                       aGCACAGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGAACGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTc                                                            >  1:886480/1‑149 (MQ=255)
                                                                                            cgcgTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGccc                                                       >  2:875439/1‑148 (MQ=255)
                                                                                                   gTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGAGCCTg                                                >  1:753301/1‑149 (MQ=255)
                                                                                                    tGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGc                                               <  1:347622/149‑1 (MQ=255)
                                                                                                      ccGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCa                                             <  1:554918/149‑1 (MQ=255)
                                                                                                         tCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATCGTCCGCCAGATCCTGCCAGGt                                          >  2:173712/1‑149 (MQ=255)
                                                                                                         tCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCCGATCCTGCCAGGt                                          >  1:111767/1‑149 (MQ=255)
                                                                                                         tCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGAATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATATTCCGCCAGAGCCTGCCCGGt                                          >  1:333647/1‑149 (MQ=255)
                                                                                                            aaGCCGTTTTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGtttt                                       <  1:61930/149‑1 (MQ=255)
                                                                                                                cGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTTCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTGCAGGTTCGCGGCATAGTCCGCCGGATCCTGCCAGGttttgtgc                                   >  1:498383/1‑145 (MQ=255)
                                                                                                                 gttgttTTTGCTGGCAAACGGCAGACCGTTCCGGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTgg                                                                                                          >  2:299166/1‑77 (MQ=255)
                                                                                                                 gttgttTTTGCTGGCAAACGGCAGACCGTTCCGGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTgg                                                                                                          <  1:299166/77‑1 (MQ=255)
                                                                                                                 gttgttTTTGCTGGCAAACGGCAGACCGTTCCGGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTgg                                                                                                          <  1:11505/77‑1 (MQ=255)
                                                                                                                 gttgttTTTGCTGGCAAACGGCAGACCGTTCCGGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTgg                                                                                                          >  2:11505/1‑77 (MQ=255)
                                                                                                                 cttgttTTGGCTGGCAAGCGGCAGTCCGTTCCAGGCGCTAAAGTTTTCCAGGTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGTTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTcggcg                                  <  2:271732/148‑1 (MQ=255)
                                                                                                                           cTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCgg                        <  1:188623/149‑1 (MQ=255)
                                                                                                                                    ggCAGTCCGGTCCAGGGGCTAAAGTTTTCCATTTGGATCCAGCGCTGCCAGCCGCTGGCGTAGCCGGATTTCATGCCGGGGGCTTTCAGTTTCGCGGCATAGTCGGGCAGTTCCTGCGAGGTTTTCGGCGGCTGTTTCGGGTCtcttgc               <  2:373662/149‑6 (MQ=255)
                                                                                                                                           cGTTCCAGGCGCTAAAGGTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTc        <  1:762254/149‑1 (MQ=255)
                                                                                                                                             ttCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCtt      >  2:178867/1‑149 (MQ=255)
                                                                                                                                               ccAGGCGATAAAGTTTTCCAGTTGGTTTCTGTGTTGACAGACGCTGGCGTATCTGCACTTCATCCCGGATTCCTTCATTTTCTCTCCATACTCTCGCAGATTCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGa    <  2:82129/149‑1 (MQ=255)
                                                                                                                                                 aGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCAGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAg  >  2:534384/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCATCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAG  >  NC_000913_3_pae_tpiA/3591571‑3591864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑