| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 1,026,965 | C→G | 12.2% | V17V (GTG→GTC) | mgsA ← | methylglyoxal synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 1,026,965 | 0 | C | G | 12.2% | 83.2 / 8.1 | 33 | V17V (GTG→GTC) | mgsA | methylglyoxal synthase |
| Reads supporting (aligned to +/- strand): ref base C (14/15); new base G (2/2); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.52e-01 | |||||||||||
GGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAG > NC_000913_3_pae_tpiA/1026817‑1027099 | ggCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGCGTTGCTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGcc > 2:574526/1‑149 (MQ=255) tCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAgc > 2:717563/1‑149 (MQ=255) tCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAgc < 1:526892/149‑1 (MQ=255) tCGCGGTGACGTTCATGCCGGGCTCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAata < 2:548655/149‑1 (MQ=255) gACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTcc < 2:712736/149‑1 (MQ=255) gTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCcgcg > 1:283378/1‑149 (MQ=255) ttCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTGCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCCGCGCAATAGAGTTCCgcgc > 1:286787/1‑149 (MQ=255) cATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAg > 2:490070/1‑149 (MQ=255) tCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCATCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTg < 2:471711/149‑1 (MQ=255) cgctGGAAATTAAGCTACTGGTATGGCCTGTTGCATTCAGTACGGCTTGTTCCAGGAACGGTTGATGCCGTTCCACCCAGCCCATCAGCATTTGTTGGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGa < 1:305395/149‑1 (MQ=255) gAAATTAAGTTACCGGGAGTGCCTGTTGCATACAGGACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAgtcgt < 1:846644/149‑1 (MQ=255) aTTAAGTTACCGGTAGGGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAg < 2:867021/149‑1 (MQ=255) tGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTa > 1:407708/1‑149 (MQ=255) aGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTc < 1:167365/149‑1 (MQ=255) gtgtTGTTCCAGTAACGGTTGATACCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTCCGAGTCGTCAGTTCCCTAATGTACAACCGACGTTAACTTTCCTACa > 2:424233/1‑149 (MQ=255) cAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTCCAGCTTGCTGt > 1:308777/1‑149 (MQ=255) aGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGTAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTACCTTTCCTACAGATTACTGTa > 2:175918/1‑149 (MQ=255) tAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAg > 1:272572/1‑149 (MQ=255) cGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCAc > 2:736400/1‑149 (MQ=255) gTTTATGCCGTTCCACCCATCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACtt < 2:208417/149‑1 (MQ=255) gTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACtt > 2:871000/1‑149 (MQ=255) ttGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTa > 1:781435/1‑149 (MQ=255) gATGTCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATc < 1:669200/149‑1 (MQ=255) ttCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCaa < 2:407708/149‑1 (MQ=255) ccACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCCTAATGTACACCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGa < 1:241886/149‑1 (MQ=255) ccAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCGACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGa < 1:318748/73‑1 (MQ=255) ccAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCGACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGa > 2:318464/1‑73 (MQ=255) ccAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCGACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGa > 2:318748/1‑73 (MQ=255) ccAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCGACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGa < 1:318464/73‑1 (MQ=255) tcatcaGCATTTGTTTGCAGTGATCGTGGGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCg < 1:178657/149‑1 (MQ=255) catcaGCGTTTGTTTGCAGCGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGa < 2:235189/149‑1 (MQ=255) atcaGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGaa > 1:196985/1‑149 (MQ=255) aGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGaaaaa > 2:894258/1‑149 (MQ=255) cAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAg < 1:708471/149‑1 (MQ=255) | GGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAG > NC_000913_3_pae_tpiA/1026817‑1027099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |