Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA475,9720CG21.3% 337.1 / 92.3 136intergenic (+21/+10)ybaY/ybaZouter membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
Reads supporting (aligned to +/- strand):  ref base C (53/54);  new base G (15/14);  total (68/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 138 total aligned reads displayed.

TGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCGGT  >  NC_000913_3_pae_tpiA/475830‑476121
                                                                                                                                              |                                                                                                                                                     
tGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCACCTCAGGTGAATCCGTCTTCGTCAGTTCCAGCTCCGACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGagag                                                                                                                                                <  4:638427/150‑1 (MQ=255)
 gCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAgg                                                                                                                                               <  1:1366629/150‑1 (MQ=255)
  cgggTACAGCAAACCGCCGTGCCGGTTCGGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAAGCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                              <  2:251135/148‑1 (MQ=255)
  ccGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                              <  2:250710/150‑1 (MQ=255)
  ccGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                              <  4:190086/150‑1 (MQ=255)
   cGGTACAGCAAACCGCCGTGACGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGt                                                                                                                                             <  2:1032449/150‑1 (MQ=255)
    ggTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGtt                                                                                                                                            <  2:615563/150‑1 (MQ=255)
    ggTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGtt                                                                                                                                            >  1:365619/1‑150 (MQ=255)
      tACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtag                                                                                                                                          <  4:599478/150‑1 (MQ=255)
         aGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                              <  2:1004797/143‑1 (MQ=255)
         aGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                              >  1:1004797/1‑143 (MQ=255)
          gCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGTtaacgtc                                                                                                                                      <  2:330194/150‑6 (MQ=255)
           cAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGTtaacgtcg                                                                                                                                     >  1:1056195/1‑144 (MQ=255)
           cAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGTtaacgt                                                                                                                                       >  3:564529/1‑144 (MQ=255)
           cAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGTtaacgt                                                                                                                                       <  4:564529/148‑5 (MQ=255)
           cAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTta                                                                                                                                           >  2:524795/1‑144 (MQ=255)
           cAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGtt                                                                                                                                     >  1:306447/1‑150 (MQ=255)
                     tGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTCCGCCAAATTAAGCCACGTTAACATTCTCCGACCTTACGGTTTGAGTAGTGCCAGCGATag                                                                                                                           >  1:1026495/1‑149 (MQ=255)
                       ccGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                              >  1:95799/1‑129 (MQ=255)
                         ggTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCt                                                                                                                       <  3:195948/150‑1 (MQ=255)
                             cAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGTTATCGCTGGaa                                                                                                                   <  2:365619/150‑1 (MQ=255)
                               ggCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTc                                                                                                                                    >  2:1143450/1‑131 (MQ=255)
                               ggCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTc                                                                                                                                    <  1:1143450/131‑1 (MQ=255)
                                  cAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAgg                                                                                                                                               <  1:253263/117‑1 (MQ=255)
                                    gCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGAtt                                                                                                            <  2:1407874/150‑1 (MQ=255)
                                       gTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTta                                                                                                                                           <  3:525029/116‑1 (MQ=255)
                                          gcgcAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGtt                                                                                                                                            <  2:239781/112‑1 (MQ=255)
                                             cAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGtt                                                                                                                                            <  1:502288/109‑1 (MQ=255)
                                             cAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGtt                                                                                                                                            >  2:502288/1‑109 (MQ=255)
                                                cGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAGCGCTGCAAGTCGATTTGCCCGCTTccc                                                                                                <  3:212743/150‑1 (MQ=255)
                                                  aCTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAGGCGACGTTAACCCTCTCCGCCCGGAGAGTGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGa                                                                                              <  1:1115936/150‑1 (MQ=255)
                                                  aCTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGa                                                                                              <  4:493841/150‑1 (MQ=255)
                                                  aCTACCATACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCATCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGa                                                                                              <  2:971951/150‑1 (MQ=255)
                                                       cGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCa                                                                                         >  4:2037/1‑150 (MQ=255)
                                                           ccTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATCAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATcac                                                                                     >  3:690913/1‑150 (MQ=255)
                                                              tCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                              <  2:900340/90‑1 (MQ=255)
                                                                  cTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTc                                                                               <  2:394691/149‑1 (MQ=255)
                                                                  cTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCt                                                                              <  2:193240/150‑1 (MQ=255)
                                                                   ttCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTg                                                                             >  2:201412/1‑150 (MQ=255)
                                                                      aCCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGgaga                                                                                                                                                 <  4:704450/79‑1 (MQ=255)
                                                                      aCCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCt                                                                              <  2:1192312/146‑1 (MQ=255)
                                                                      aCCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCt                                                                              >  1:1192312/1‑146 (MQ=255)
                                                                       ccAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAg                                                                         >  1:1366571/1‑150 (MQ=255)
                                                                       ccAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAg                                                                         <  1:1134077/150‑1 (MQ=255)
                                                                         aaCTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTa                                                                       >  1:893514/1‑150 (MQ=255)
                                                                            tCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATg                                                                    >  1:463568/1‑150 (MQ=255)
                                                                              aGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGcc                                                                           <  1:630287/141‑1 (MQ=255)
                                                                               ggTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCt                                                                 <  4:281345/150‑1 (MQ=255)
                                                                                  gAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt                                                                                                                             >  4:695898/1‑87 (MQ=255)
                                                                                  gAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt                                                                                                                             <  3:695898/87‑1 (MQ=255)
                                                                                  gAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCaa                                                                                                                   <  2:1325279/97‑1 (MQ=255)
                                                                                  gAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTc                                                              >  1:479641/1‑150 (MQ=255)
                                                                                   aaTCCGTCTTTGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCg                                                             <  2:390164/150‑1 (MQ=255)
                                                                                    aTCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGc                                                                            <  3:299759/134‑1 (MQ=255)
                                                                                        gTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGAc                                                        >  2:1338686/1‑150 (MQ=255)
                                                                                            tCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGTtaac                                                                                                                                         <  2:237434/65‑3 (MQ=25)
                                                                                            tCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGTtaac                                                                                                                                         >  1:237434/1‑63 (MQ=37)
                                                                                            tCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTg                                                    <  2:1047517/150‑1 (MQ=255)
                                                                                             cGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATcac                                                                                     >  1:1446521/1‑116 (MQ=255)
                                                                                              ggCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAgg                                                                                                                                               >  2:292555/1‑57 (MQ=255)
                                                                                              ggCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGt                                                                                                                 <  2:173947/87‑1 (MQ=255)
                                                                                              ggCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGt                                                                                                                 >  1:173947/1‑87 (MQ=255)
                                                                                               gCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGc                                                                                                     >  1:126428/1‑98 (MQ=255)
                                                                                               gCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGc                                                                                                     <  2:126428/98‑1 (MQ=255)
                                                                                               gCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCg                                                             <  1:642454/138‑1 (MQ=255)
                                                                                               gCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCg                                                             >  2:642454/1‑138 (MQ=255)
                                                                                                     ccAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATa                                                                                            >  3:703897/1‑101 (MQ=255)
                                                                                                             tACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTa                                   <  2:1366571/150‑1 (MQ=255)
                                                                                                              aCGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAg                                  >  1:338688/1‑150 (MQ=255)
                                                                                                               cGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTaa                                                 <  1:1464475/134‑1 (MQ=255)
                                                                                                               cGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTaa                                                 >  2:1464475/1‑134 (MQ=255)
                                                                                                                     atTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGa                                                                                                                              >  2:1158404/1‑51 (MQ=255)
                                                                                                                          gCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATtgtgac                      <  4:690913/150‑3 (MQ=255)
                                                                                                                            gACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGc                                                                   <  2:656797/103‑1 (MQ=255)
                                                                                                                             ctactAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGt                                                   <  1:695633/114‑1 (MQ=255)
                                                                                                                             ctactAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGt                                                   >  2:695633/5‑118 (MQ=255)
                                                                                                                             aCGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTg                   <  1:240569/150‑1 (MQ=255)
                                                                                                                              tactAACCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGa                                                         >  2:930338/4‑111 (MQ=255)
                                                                                                                              tactAACCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGa                                                         <  1:930338/108‑1 (MQ=255)
                                                                                                                                ctAACCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCt                                                     >  3:841927/2‑113 (MQ=255)
                                                                                                                                ctAACCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCt                                                     <  4:841927/112‑1 (MQ=255)
                                                                                                                                 tAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGa               <  3:862769/150‑1 (MQ=255)
                                                                                                                                  aaCCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGAt              >  2:1386712/1‑150 (MQ=255)
                                                                                                                                   aCCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGAtt             >  2:174035/1‑150 (MQ=255)
                                                                                                                                   aCCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGAtt             >  2:174100/1‑150 (MQ=255)
                                                                                                                                   aCCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATa                                                                                            >  2:1446526/1‑71 (MQ=255)
                                                                                                                                   aCCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATa                                                                                            <  1:1446526/71‑1 (MQ=255)
                                                                                                                                   aCCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGAtt             <  1:1363296/150‑1 (MQ=255)
                                                                                                                                    cccTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTa            >  2:1423763/1‑150 (MQ=255)
                                                                                                                                    cccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTcc                                                                                                 <  2:245102/65‑1 (MQ=255)
                                                                                                                                    cccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTcc                                                                                                 >  1:245102/1‑65 (MQ=255)
                                                                                                                                    cccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTa            >  1:446698/1‑150 (MQ=255)
                                                                                                                                     ccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTaa           <  4:487489/150‑1 (MQ=255)
                                                                                                                                     ccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTaa           >  2:1440790/1‑150 (MQ=255)
                                                                                                                                      ctctCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATtgtg                        >  3:332622/1‑136 (MQ=255)
                                                                                                                                      ctctCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATtgtg                        <  4:332622/136‑1 (MQ=255)
                                                                                                                                         tcCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAaccac       >  3:516183/1‑150 (MQ=255)
                                                                                                                                           cGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAg                                                                         >  1:430710/1‑82 (MQ=255)
                                                                                                                                            gCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCg    >  1:816042/1‑150 (MQ=255)
                                                                                                                                              ccGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCGGt  >  2:18726/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
TGCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAACCACCCGGT  >  NC_000913_3_pae_tpiA/475830‑476121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑