New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 2897909	34 (1.180)	5 (0.190)	5/268	3.1	13.5%	intergenic (‑39/+55)	ygcS/ygcU	putative MFS sugar transporter; membrane protein/putative FAD‑linked oxidoreductase
	?	NC_000913_3_pae_tpiA	= 2897913	32 (1.190)					intergenic (‑43/+51)	ygcS/ygcU	putative MFS sugar transporter; membrane protein/putative FAD‑linked oxidoreductase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGAACATAACCGTCGGTCAGGTGTGCGCCGAAAGTGAGCGCAGCAATGCGGCAGTGAAAACGGTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCAACGCCCACGCGCTGCC  >  NC_000913_3_pae_tpiA/2897767‑2898056                                                                                                                                               |                                                                                                                                                    agaACATAACCGTCGGTCAGGTGTGCGCCGAAAGTGAGCGCAGCAATGCGGCAGTGAAAACGGTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTga                                                                                                                                               >  1:467090/1‑149 (MQ=255)       tAACCGTCGGTCAGGTGGGCGCCGAAAGTGAGCGCAGCAATGCGGCAGTGAAAACGGTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGcc                                                                                                                                         <  1:386900/149‑1 (MQ=255)             tCGGTCAGGTGTGCGCCGAAAGTGAGCGCAGCAATGCGGCAGTGAAAACGGTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAg                                                                                                                                   <  2:419467/149‑1 (MQ=255)                     gtgtGCGCCGAAAGTGAGCGCAGCAATGCGGCAGTGAAAACGGTTAAGCGGGAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATCGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGcc                                                                                                                           <  1:25664/149‑1 (MQ=255)                                   tGAGCCCAGCAATGCGGCAGTGAAAACGGTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGaa                                                                                                             <  1:481250/149‑1 (MQ=255)                                        gcagcaATGCGGCAGTGAAAACGGTTAAGCGGTAAATCATCCATTAGCACCGGTGAAGTGTTCATCCTTACCTCTTCATTGTCCGGTAATAAGAACGGCTTCTGGCCTGAGAAGCGGAAAAGGCAACGCCCCGTCATCGCGGAACGCTg                                                                                                        >  1:184236/1‑149 (MQ=255)                                           gcaATGCGGCAGTGAAAACGGTTAAGCGGTAAATCGTCCATTCGCCCCGGTGAAGTGTTCATCTTTACCCCTTCATCGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCAGCGCGGAACGCTGCCt                                                                                                     <  2:136315/149‑1 (MQ=255)                                                    catggAAAACGGTAAAGCGGTAAATCTTCCATTCGCACCGGTGAAGTGTTCATCTTTCCCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACatta                                                                                            <  2:122942/145‑1 (MQ=255)                                                       tGAAAACGGTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACACCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTAttt                                                                                         >  1:256572/1‑149 (MQ=255)                                                              ggTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAAta                                                                                  <  2:467090/149‑2 (MQ=255)                                                                 tAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGga                                                                               <  1:441143/149‑1 (MQ=255)                                                                    gCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGgatag                                                                            >  1:491652/1‑149 (MQ=255)                                                                    gCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGgatag                                                                            >  1:478379/1‑149 (MQ=255)                                                                    gCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCATTCAATGGCGGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGgatag                                                                            >  1:397818/1‑149 (MQ=255)                                                                     cgttAAACCATCCATGCGCACCGGAGAAGGGTTCATCTTTACCCCCTCATTGTCCGGTAATAAGAACGGCTTCCCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGgataga                                                                           <  2:307680/146‑1 (MQ=255)                                                                      gttAAATCATCCATTCGCACCGGTGACATGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGCAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACTTTATTTTTCAATCGgatagat                                                                          <  1:27873/147‑1 (MQ=255)                                                                      ggTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGgatagat                                                                          <  1:28821/149‑1 (MQ=255)                                                                       gTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCg                                                                                 >  1:49774/1‑141 (MQ=255)                                                                       gTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCg                                                                                 <  2:49774/141‑1 (MQ=255)                                                                          aaTCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTa                                                                      <  1:211604/149‑1 (MQ=255)                                                                           atcctcCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGACCGGCTTCTCGCCTGAGAAGCCGAAAAGGAAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATCGTAc                                                                     <  2:387660/149‑1 (MQ=255)                                                                            tcatcCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGTCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGCTATATAGTAcc                                                                    >  2:16503/1‑149 (MQ=255)                                                                               tcCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGt                                                                 >  1:107810/1‑149 (MQ=255)                                                                                 cATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATAGGATAGATAGTACCCGTAt                                                               <  2:426611/149‑1 (MQ=255)                                                                                     cGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCAt                                                           <  2:491652/149‑1 (MQ=255)                                                                                                    ttCATTTTTACCCCTTCAATGTCCGGTAATTAGGACGGTTTCTCGCCTGATAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATc                                            <  1:131801/149‑1 (MQ=255)                                                                                                               cccTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGAt                                             >  2:162638/1‑137 (MQ=255)                                                                                                               cccTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGAt                                             <  1:162638/137‑1 (MQ=255)                                                                                                                   tCATTGTCCGGTGATAAGAACGGCTTCTCGCGTGAGAAGCCGAAAAGGCAGCTCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAg                             <  1:357018/149‑1 (MQ=255)                                                                                                                             gTAATAAGAACGGCTTCTCGCCTCCCACGTCGCCTTGGCAACGCCCCGCCATCACGGAACACTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATACTGCCATTCGGATCTCACCGCTTTTTTGCCCCTTCCCGc                   >  2:236888/1‑149 (MQ=255)                                                                                                                                     aaCGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTAccc                                                                   <  2:362934/93‑1 (MQ=255)                                                                                                                                     aaCGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTAccc                                                                   >  1:362934/1‑93 (MQ=255)                                                                                                                                          cTTCTCGCCGGAGAAGGCTAAAAGGCTACGGCCCGTGATCGCGGAACGCTGCCTGACACATTAGTTTTCCATCGGAGAGATACTACCCGTATTCATCATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCCGCAACGCCCACgcgc      <  2:148976/149‑1 (MQ=255)                                                                                                                                              tcGCCGGAGAGGCCGAAAAGGCAACTCGCCGTCATGGCGGAACGGGGCCTGACTCATTATTTTTCAATCGGATAGATAGTAACCCTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCAACGCCCACGCGCTGcc  <  2:53872/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    AGAACATAACCGTCGGTCAGGTGTGCGCCGAAAGTGAGCGCAGCAATGCGGCAGTGAAAACGGTTAAGCGGTAAATCATCCATTCGCACCGGTGAAGTGTTCATCTTTACCCCTTCATTGTCCGGTAATAAGAACGGCTTCTCGCCTGAGAAGCCGAAAAGGCAACGCCCCGTCATCGCGGAACGCTGCCTGACACATTATTTTTCAATCGGATAGATAGTACCCGTATTCATAATGCCATTAGGATCGAACTGCTTTTTCAGCCCTTCCAGCAACGCCCACGCGCTGCC  >  NC_000913_3_pae_tpiA/2897767‑2898056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.