New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 3305605 =21 (0.730)4 (0.150) 4/272 3.6 15.4% coding (213/1245 nt) mtr tryptophan transporter of high affinity
?NC_000913_3_pae_tpiA 3305636 = 24 (0.880)coding (182/1245 nt) mtr tryptophan transporter of high affinity
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGA  >  NC_000913_3_pae_tpiA/3305511‑3305784
                                                                                                                             |                                                                                                                                                    
tCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGATTCATCGAGCCGGAATGCAGCATACAg                                                                                                                               >  2:49180/1‑149 (MQ=255)
tCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAg                                                                                                                               <  2:350188/149‑1 (MQ=255)
   ggATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAAc                                                                                                                            <  2:386890/149‑1 (MQ=255)
              acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAACGAt                                                                                                                 >  2:454142/1‑149 (MQ=255)
                          gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCAt                                                                                                     <  1:23151/149‑1 (MQ=255)
                             aTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAAc                                                                                                                            >  2:436148/1‑123 (MQ=255)
                             aTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAAc                                                                                                                            <  1:436148/123‑1 (MQ=255)
                               gCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAgc                                                                                                             <  2:70504/136‑1 (MQ=255)
                               gCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAgc                                                                                                             >  1:70504/1‑136 (MQ=255)
                                        cACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAGGATCAGCGCCGCCATTGACCAGaaaaaac                                                                                       >  1:20429/1‑147 (MQ=255)
                                        cACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATACAGGTAAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCCGGTAAAGATCAGCGCCGCCATTGACCAGAAAAAcc                                                                                       >  1:172074/1‑149 (MQ=255)
                                                                       gTGATGGTGTCAAAACCCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGaaaa                                                        <  1:331330/149‑1 (MQ=255)
                                                                                      cTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTg                                                                  <  1:288525/124‑1 (MQ=255)
                                                                                      cTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTg                                                                  >  2:288525/1‑124 (MQ=255)
                                                                                          aaCCGATTCTGTAATTTAGGTTAGCTTCCAGAAACATCAAGCTGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCTCGGAAATGATCACTGGCAGAGAAAACATCCCCGCGCCATTAATg                                     <  2:189201/149‑1 (MQ=255)
                                                                                             cGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCGTTGACCAGAAAAACCACGCCCGGGACATGACCACTGGCAGAGAAAACCTCCCTGCGCCCATAATGGtc                                  >  2:143148/1‑148 (MQ=255)
                                                                                                     tAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATcc                                                   <  1:3143/124‑1 (MQ=255)
                                                                                                     tAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATcc                                                   >  2:3143/1‑124 (MQ=255)
                                                                                                       aTTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATaa                        >  2:416414/1‑149 (MQ=255)
                                                                                                         tacggTTAGCTTCCAGAATCATCAAGGCTGAATGCAGCATACAGACCAAGGTAAAGAGCAGCGCCGCCATTGACCAGAAAATCCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATc                      <  1:115983/146‑1 (MQ=255)
                                                                                                         tCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATc                      <  1:416414/149‑1 (MQ=255)
                                                                                                            ggTTAGCTTCCAGAATCATCAAGCCCGACGGCACCATTCAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATcacc                   >  1:375835/1‑149 (MQ=255)
                                                                                                                  cTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACAActccg             >  1:32475/1‑149 (MQ=255)
                                                                                                                    tCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCAAAGCGCCAATAATGGTGCCGCAGATACTCACCCcgaagca           >  1:232460/1‑144 (MQ=255)
                                                                                                                      cAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCaa         <  1:499095/149‑1 (MQ=255)
                                                                                                                         aaTCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGc                                                                                   <  2:206398/72‑1 (MQ=255)
                                                                                                                         aaTCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGc                                                                                   >  1:206398/1‑72 (MQ=255)
                                                                                                                           tcatcaAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCAcgccgc            >  2:100348/1‑141 (MQ=255)
                                                                                                                           tcatcaAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCAcgccgc            <  1:100348/141‑1 (MQ=255)
                                                                                                                           tcatcaAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCACCGCCATTGACCAGAAAAACCACGCCCCGAACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTCCGGCCGATAATCAAGACGCCGCCAagcagc    >  1:23366/1‑149 (MQ=255)
                                                                                                                             atcaAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGCAAATCACCCCGCCGCCAAGCACCgc  >  1:252432/1‑148 (MQ=255)
                                                                                                                             |                                                                                                                                                    
TCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTGCCGCCGATAATCACCACGCCGCCAAGCAGCGA  >  NC_000913_3_pae_tpiA/3305511‑3305784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.