New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 458305328 (0.970)5 (0.180) 5/274 3.2 16.4% intergenic (‑89/+112) hsdM/hsdR DNA methyltransferase M/endonuclease R Type I restriction enzyme
?NC_000913_3_pae_tpiA = 4583140 24 (0.870)intergenic (‑176/+25) hsdM/hsdR DNA methyltransferase M/endonuclease R Type I restriction enzyme
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCCAGCAGGGTATCGAGATTATCGTCAAAGGTTCTTTGCAGCATCGCCTTCCCGCCGCGACGGTGGAAGTTGCCGGTTTTGAA  >  NC_000913_3_pae_tpiA/4583012‑4583284
                                                                                                                                 |                                                                                                                                                
aagaagaaATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGtata                                                                                                                              <  2:359404/149‑1 (MQ=255)
  gaagaaATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACACACGTACGTGACCGGCGTGAACGAGCGCATCCAAAGCAGAGGCAGCCTGAAGGATGAAGTGTATACg                                                                                                                            >  1:71953/1‑149 (MQ=37)
       aaTTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCa                                                                                                                       >  1:440633/1‑149 (MQ=255)
        agtGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAg                                                                                                                      <  2:461379/147‑1 (MQ=255)
          tGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGc                                                                                                                    <  1:354538/149‑1 (MQ=255)
                    aaTATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGACCGAGCGCAGCCCCACGGTAGCCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTcc                                                                                                          <  1:497789/149‑1 (MQ=38)
                      tataCACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCa                                                                                                        <  1:44408/149‑1 (MQ=255)
                          cacaAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGata                                                                                                    <  2:165125/149‑1 (MQ=255)
                                 tCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGc                                                                                             >  2:421032/1‑149 (MQ=255)
                                    ttCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGa                                                                                          >  1:310466/1‑149 (MQ=255)
                                              aTCAAGGCGGCAAGTGAGTGAGTCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCcag                                                                                >  1:194492/1‑149 (MQ=255)
                                                 aaGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCcagcag                                                                             >  2:52829/1‑149 (MQ=255)
                                                     cggcAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCCAGCAGGGTa                                                                         <  1:49803/149‑1 (MQ=255)
                                                        aaaGGGAGTGAATCCCCGGGAGCGTACAGAAGTCCGTGACCGGAGTGACAGAGCGCAGCGAAGGCAGAGTAATCCTGAAGGATGAAATTGATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCCAGCAGGGTATCg                                                                      <  2:373267/148‑1 (MQ=255)
                                                                    tCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCCAGCAGGGTATCGAGATTATCGTCa                                                          >  1:453093/1‑149 (MQ=255)
                                                                                           gTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCCAGCAGGGTATCGAGATTATCGTCAAAGGTTCTTTGCAGCATCGCCtt                                   <  2:193018/149‑1 (MQ=255)
                                                                                                      gAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGCTATAATCGCTGAATTTGCCCAGCAGGGTATCGAGATTATCGTCAAAGGTTCTTTGAAACATACACTCCCcgccgctcc                        >  2:72842/1‑146 (MQ=255)
                                                                                                        aCGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCCAGCAGGGTATCGAGATTATCGTCAAAGGTTCTTTGCAGCATCGCCTTCCCGCCGCGACgg                      <  2:418368/149‑1 (MQ=255)
                                                                                                             cgcAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCCAGCAGGGTATCGAGATTATCGTCAAAGGTTCTTTGCAGCATCGCCTTCCCGCCGCGACGGTGGaa                 <  1:421032/149‑1 (MQ=255)
                                                                                                                aGCGAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGTCGAATTTTCCTAGCAGGTTATCGAGATTATCGTCAAAGGTTCTTTGCAGCATCGCCTTCCCGCCGCGACGGTGGAAGtt              <  2:56740/149‑1 (MQ=255)
                                                                                                                 gCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCAAGATATAATCGCTGAATTTGCCCAGCAGGGTATCGAGATTATCGTCAAAGGTTCTTTGCAGCATCCCCTTCCCCCCGCGACCGGGGAAGtgg             >  1:133681/1‑147 (MQ=255)
                                                                                                                    aaaggCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCCAGCAGGGTATCGAGATTATCGTCAAAGGTTCTTTGCAGCATCGCCTTCCCGCCGCGACGGTGGAAGTTGcc           <  2:78578/145‑1 (MQ=255)
                                                                                                                             ggCAGTCTGAAGGATGAAGTGGATACGTGTCAGTCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCCAGCAGGGTATCGAGATTATCGTCAAAGGTTCTTTGCATCATCGCCTTCCCGCCGCGACGGTGGAAGTTGCCGGTTTTGaa  <  2:310466/149‑1 (MQ=255)
                                                                                                                             ggCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCCAGCAGGGTATCGAGATTATCGTCAAAGGTTCTTTGCAGCATCGCCTTCCCGCCGCGACGGTGGAAGTTGCCGGTTTTGaa  <  1:339538/149‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                                
AAGAAGAAATTGCCGAGGGTAATATACACAAAATCATTCAGGTTGCATCAAGGCGGCAAGTGAGTGAATCCCCGGGAGCGTACAGAAGTACGTGACCGGGGTGAACGAGCGCAGCCAACGCAGAGGCAGCCTGAAGGATGAAGTGTATACGTGTCAGGCCAGCTCGTCCCAGATATAATCGCTGAATTTGCCCAGCAGGGTATCGAGATTATCGTCAAAGGTTCTTTGCAGCATCGCCTTCCCGCCGCGACGGTGGAAGTTGCCGGTTTTGAA  >  NC_000913_3_pae_tpiA/4583012‑4583284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.