Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1299499–1300697 1300697 1–1199 36 [0] [0] 36 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)35 (1.250) 34/278 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  NC_000913_3_pae_tpiA/1300550‑1300843
                                                                                                                                                    |                                                                                                                                                 
ttctcaaattacagttagttataaggatttccttaactgattctcctcaccattatgttattttcgccacctcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                   >  1:198013‑M2/149‑149 (MQ=255)
   tcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcatttgttagaggaaagtgctaaatat                                                                                                                                                >  2:356971‑M2/146‑148 (MQ=255)
                 gttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                                  >  2:137516‑M2/132‑149 (MQ=255)
                  ttataaggatttccttaactgcttctcctcaccatcatgttattttggccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaattgctaaATAATAATCAATTGTTaaa                                                                                                                                 >  2:185102‑M2/131‑149 (MQ=255)
                    ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatt                                                                                                                               >  1:22486‑M2/129‑149 (MQ=255)
                       aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                            <  1:52015‑M2/24‑1 (MQ=255)
                       aggatttccttaactgcgtctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgacgaataattgaaatgatattattaattccactgcctttggtagaggaaagtcctaaATAATAATCAATTGTTAAatcatt                                                                                                                            >  2:219399‑M2/126‑149 (MQ=255)
                          atttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagattaattgaaatgatattattaattacactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtctg                                                                                                                         >  2:162987‑M2/123‑149 (MQ=255)
                          atttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                         <  2:4851‑M2/27‑1 (MQ=255)
                                    ctgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                               <  1:160589‑M2/37‑1 (MQ=255)
                                      gcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                                    <  2:71262‑M2/16‑1 (MQ=255)
                                      gcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                                    >  1:71262‑M2/111‑126 (MQ=255)
                                       cttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                            <  1:491292‑M2/40‑1 (MQ=255)
                                         tctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaactgatattattaattccactgcctttggtataggaaagtgctaaATAATAATCAATTGTTAAATTATTTTGCATTTCACTACTAGa                                                                                                          >  2:406615‑M2/108‑149 (MQ=255)
                                                 ccatcatgttattgttgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                                  <  2:281537‑M2/50‑1 (MQ=255)
                                                  catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                                 <  1:72439‑M2/51‑1 (MQ=255)
                                                       tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGGACTGTAAt                                                                                                  <  2:104369‑M2/50‑1 (MQ=255)
                                                       tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                                  >  1:104369‑M2/94‑143 (MQ=255)
                                                         ttatcttctccacatcataatcttgggcttgctgaagaataattgaaatgatattagtaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAg                                                                                          <  2:450305‑M2/58‑1 (MQ=255)
                                                                 gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                                  <  2:22486‑M2/66‑1 (MQ=255)
                                                                  ccaaatcataatcctgggcttgctgaagaataattgaaatgatagtattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                 <  1:382348‑M2/67‑1 (MQ=255)
                                                                                      tgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttga                                                             <  2:346970‑M2/87‑1 (MQ=255)
                                                                                         tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGACCTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgattg                                                          >  1:167793‑M2/60‑149 (MQ=255)
                                                                                           aagaagaagtgaaatgatattattaattccactgcctttggtagaggaaagtgcaaaATAATAATCAATTGTTAAATTATTGTGCAATTCACTACTGGAACTGTAATCATAAAAGTTAGACATGCTTAGCCAATCTCTATTTGatagaa                                                        <  2:463605‑M2/92‑1 (MQ=255)
                                                                                             gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaatt                                                      <  1:165827‑M2/94‑1 (MQ=255)
                                                                                              aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTCGCCAATCTCTATTTGgttgaattg                                                     >  2:238952‑M2/55‑149 (MQ=255)
                                                                                                  attgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAg                                                 >  1:137903‑M2/51‑149 (MQ=255)
                                                                                                        aagatattattatatccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtggtt                                           <  2:63509‑M2/105‑1 (MQ=255)
                                                                                                          gatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                         >  2:202729‑M2/43‑149 (MQ=255)
                                                                                                           atataaataattccacagcctttggtagaggaaagtgctaaATAATAATCAATTGATAAAATATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                        <  2:3330‑M2/108‑1 (MQ=255)
                                                                                                                attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGc                                   >  2:54074‑M2/37‑149 (MQ=255)
                                                                                                                     ttccactgcctttagtagaggaaagtactaaATAATAATCAATTGTTAAAGTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGa                              >  1:414791‑M2/32‑149 (MQ=255)
                                                                                                                           tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAt                                                                 <  2:270918‑M2/83‑1 (MQ=255)
                                                                                                                           tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAt                                                                 >  1:270918‑M2/26‑108 (MQ=255)
                                                                                                                                      gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTCGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt             >  1:231540‑M2/15‑149 (MQ=255)
                                                                                                                                                 taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCaa  >  1:147975‑M2/4‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAA  >  NC_000913_3_pae_tpiA/1300550‑1300843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.