Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 3,440,923 C→T G36D (GGC→GAC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,440,9230CT94.7% 430.6 / 8.9 131G36D (GGC→GAC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base T (58/66);  total (62/69)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 131 total aligned reads displayed.

GTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAA  >  NC_000913_3_pae_tpiA/3440778‑3441067
                                                                                                                                                 |                                                                                                                                                
gTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCaaa                                                                                                                                              <  1:1177791/150‑1 (MQ=255)
    aGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCa                                                                                                                                          <  4:306980/150‑1 (MQ=255)
    aGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCa                                                                                                                                          <  4:306963/150‑1 (MQ=255)
          aTTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGctct                                                                                                                                    <  3:537401/150‑1 (MQ=255)
           tttCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGctct                                                                                                                                    >  2:1538038/1‑149 (MQ=255)
             tCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTaaa                                                                                                                                 <  1:260052/150‑1 (MQ=255)
              ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAg                                                                                                                                <  2:1212227/150‑1 (MQ=255)
               cAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAgg                                                                                                                               <  2:1165076/150‑1 (MQ=255)
                      tCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                        >  2:751801/1‑150 (MQ=255)
                        ttCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGt                                                                                                                      <  1:138582/150‑1 (MQ=255)
                         tCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                     >  4:32063/1‑150 (MQ=255)
                                cGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAg                                                                                                                                >  1:155578/1‑132 (MQ=255)
                                cGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAg                                                                                                                                <  2:155578/132‑1 (MQ=255)
                                     tcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgc                                                                                                         >  2:877386/1‑150 (MQ=255)
                                         tGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCg                                                                                                     >  4:844238/1‑150 (MQ=255)
                                          ggTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGa                                                                                                    >  3:782462/1‑150 (MQ=255)
                                              cTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGctct                                                                                                                                    <  3:297464/114‑1 (MQ=255)
                                               tGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCa                                                                                               <  1:877386/150‑1 (MQ=255)
                                                    tCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                     <  2:1004439/123‑1 (MQ=255)
                                                    tCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTc                                                                                                                     >  1:1004439/1‑123 (MQ=255)
                                                    tCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGc                                                                                          >  2:1162432/1‑150 (MQ=255)
                                                      aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                        <  3:32063/150‑1 (MQ=255)
                                                      aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                        >  2:908698/1‑150 (MQ=255)
                                                      aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                        <  3:403395/150‑1 (MQ=255)
                                                      aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                        >  3:130468/1‑150 (MQ=255)
                                                       tGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCg                                                                                       >  1:120672/1‑150 (MQ=255)
                                                        gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                        <  2:1397578/148‑1 (MQ=255)
                                                        gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                        >  1:1397578/1‑148 (MQ=255)
                                                          agtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGata                                                                                    >  1:1006362/1‑150 (MQ=255)
                                                            taCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCa                                                                                               <  4:862300/137‑1 (MQ=255)
                                                             acacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCa                                                                                 >  2:1286807/1‑150 (MQ=255)
                                                              cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAgg                                                                                                                               >  1:253171/1‑103 (MQ=255)
                                                              cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAgg                                                                                                                               <  2:253171/103‑1 (MQ=255)
                                                                 cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGc                                                                                                                                       <  2:1112021/92‑1 (MQ=255)
                                                                 cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGc                                                                                                                                       >  1:1112021/1‑92 (MQ=255)
                                                                 cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                             >  3:935695/1‑150 (MQ=255)
                                                                 cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                             >  3:840203/1‑150 (MQ=255)
                                                                  aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGaa                                                                                                   <  2:1155965/127‑1 (MQ=255)
                                                                  aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGaa                                                                                                   >  1:1155965/1‑127 (MQ=255)
                                                                    cAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCAc                                                                                                                   >  1:1057713/1‑109 (MQ=255)
                                                                    cAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCAc                                                                                                                   <  2:1057713/109‑1 (MQ=255)
                                                                    cAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGa                                                                                                    <  2:420405/124‑1 (MQ=255)
                                                                    cAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGa                                                                                                    >  1:420405/1‑124 (MQ=255)
                                                                        ctcAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCgg                                                                      <  4:782462/150‑1 (MQ=255)
                                                                        ctcAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTCCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                        >  3:811382/1‑132 (MQ=255)
                                                                         tcAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAc                                                                               >  2:981149/1‑140 (MQ=255)
                                                                         tcAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGt                                                                     >  3:608454/1‑150 (MQ=255)
                                                                         tcAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGt                                                                     >  3:248439/1‑150 (MQ=255)
                                                                         tcAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGGGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTAACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAc                                                                               <  1:981149/140‑1 (MQ=255)
                                                                             ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTa                                                                 <  3:916376/150‑1 (MQ=255)
                                                                             ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTa                                                                 >  2:881642/1‑150 (MQ=255)
                                                                               tCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                        >  1:638426/1‑93 (MQ=255)
                                                                               tCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                        <  2:638426/93‑1 (MQ=255)
                                                                               tCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGa                                                               >  2:122549/1‑150 (MQ=255)
                                                                                cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCg                                                                                       >  1:802239/1‑125 (MQ=255)
                                                                                     aCCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                         <  1:586770/150‑1 (MQ=255)
                                                                                     aCCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                         <  4:248439/150‑1 (MQ=255)
                                                                                      ccGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTg                                                        <  4:608454/150‑1 (MQ=255)
                                                                                          gcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcac                                                    >  1:49054/1‑150 (MQ=255)
                                                                                           cAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                   <  3:844238/150‑1 (MQ=255)
                                                                                           cAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                   <  1:949279/150‑1 (MQ=255)
                                                                                               ccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAc                                               >  4:762550/1‑150 (MQ=255)
                                                                                                cGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAcc                                              >  2:935455/1‑150 (MQ=255)
                                                                                                cGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAcc                                              <  2:1363156/150‑1 (MQ=255)
                                                                                                   cATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTg                                           <  4:783245/150‑1 (MQ=255)
                                                                                                   cATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTg                                           >  2:1382505/1‑150 (MQ=255)
                                                                                                     tCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCa                                         >  3:73186/1‑150 (MQ=255)
                                                                                                       gATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAtt                                       <  3:762550/150‑1 (MQ=255)
                                                                                                        aTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGttt                                                                   >  1:680033/1‑121 (MQ=255)
                                                                                                            gagCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                  >  3:48633/1‑150 (MQ=255)
                                                                                                              acAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                <  4:321600/149‑1 (MQ=255)
                                                                                                                 gAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAcc                                                                              <  3:126951/101‑1 (MQ=255)
                                                                                                                 gAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAAcc                                                                              >  4:126951/1‑101 (MQ=255)
                                                                                                                    tACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt                          >  2:764328/1‑150 (MQ=255)
                                                                                                                     aCGGCGCAGTGCGTTACCGAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGTTATCAACCAGGCGCGGTTTTAGAACCTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTg                         <  1:107111/150‑1 (MQ=255)
                                                                                                                      cGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTgg                        <  2:49054/150‑1 (MQ=255)
                                                                                                                      cGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTgg                        <  4:383717/150‑1 (MQ=255)
                                                                                                                       ggCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTgg                        <  1:1310210/149‑1 (MQ=255)
                                                                                                                           cAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTa                   <  3:738380/150‑1 (MQ=255)
                                                                                                                             gTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                             <  2:36864/90‑1 (MQ=255)
                                                                                                                             gTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                             >  1:36864/1‑90 (MQ=255)
                                                                                                                             gTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAg                 <  1:1286807/150‑1 (MQ=255)
                                                                                                                                cGTTACCCTGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCGCTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTTACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCtt              <  4:238237/150‑1 (MQ=255)
                                                                                                                                 gTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCaa                                                                                                                          >  2:1545377/1‑41 (MQ=255)
                                                                                                                                 gTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCaa                                                                                                                          <  1:1545377/41‑1 (MQ=255)
                                                                                                                                  ttACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTa            >  4:674882/1‑150 (MQ=255)
                                                                                                                                   tACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAAct                                                           <  2:629961/102‑1 (MQ=255)
                                                                                                                                   tACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAAct                                                           >  1:629961/1‑102 (MQ=255)
                                                                                                                                   tACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTAc           <  1:1457792/150‑1 (MQ=255)
                                                                                                                                     cccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCCTTGGTACTAAGCTTTACtt         <  4:492642/150‑1 (MQ=255)
                                                                                                                                      ccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtcc        >  4:4274/1‑148 (MQ=255)
                                                                                                                                       cAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtcca       >  2:728687/1‑147 (MQ=255)
                                                                                                                                        agagTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa      <  4:840203/150‑1 (MQ=255)
                                                                                                                                        agagTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa      <  3:674882/150‑1 (MQ=255)
                                                                                                                                        agagTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa      <  2:1006362/150‑1 (MQ=255)
                                                                                                                                         gagTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGtt                                                                    >  4:715074/1‑87 (MQ=255)
                                                                                                                                            tATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACtccaagta   >  2:1100015/1‑142 (MQ=255)
                                                                                                                                            tATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTaa  >  2:509045/1‑150 (MQ=255)
                                                                                                                                             aTGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTc                                                                                                      >  4:224782/1‑49 (MQ=255)
                                                                                                                                             aTGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTc                                                                                                      <  3:224782/49‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAA  >  NC_000913_3_pae_tpiA/3440778‑3441067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: