Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 3,853,806 T→C intergenic (+164/‑116) tisB → / → emrD toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,853,8060TC94.8% 372.3 / 4.9 116intergenic (+164/‑116)tisB/emrDtoxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (3/3);  new base C (62/47);  total (65/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

AAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTT  >  NC_000913_3_pae_tpiA/3853662‑3853950
                                                                                                                                                |                                                                                                                                                
aaaGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCa                                                                                                                                             >  4:189247/1‑150 (MQ=255)
      gAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTg                                                                                                                                       >  2:1319352/1‑150 (MQ=255)
         cGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCg                                                                                                                                    >  1:449216/1‑150 (MQ=255)
           ggAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCATCATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGta                                                                                                                                  <  3:656455/150‑1 (MQ=255)
            tttcccGGTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGtat                                                                                                                                 >  4:716432/5‑150 (MQ=255)
            gAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTc                                                                                                                                          >  1:1014841/1‑141 (MQ=255)
            gAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTc                                                                                                                                          <  2:1014841/141‑1 (MQ=255)
                  tcTCCCCTTTGATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTTCCGCTTTTGTTTACATATTCTGCCGCTAAACAAGCCCCCATTCCTTGCGTATATCTgg                                                                                                                           <  4:508166/149‑1 (MQ=255)
                  gCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTgg                                                                                                                           <  1:230445/150‑1 (MQ=255)
                       ccTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCt                                                                                                                             <  2:301188/143‑1 (MQ=255)
                       ccTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCt                                                                                                                             >  1:301188/1‑143 (MQ=255)
                        cTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACa                                                                                                                     >  3:528330/1‑150 (MQ=255)
                         ttttATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACAt                                                                                                                    >  1:502662/1‑150 (MQ=255)
                          tttATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCCGGCTAACAtt                                                                                                                   >  2:38065/1‑150 (MQ=255)
                           ttATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATtc                                                                                                                  >  1:747793/1‑150 (MQ=255)
                           ttATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATtc                                                                                                                  <  2:1129743/150‑1 (MQ=255)
                              tatTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATtcatc                                                                                                               <  2:199611/150‑1 (MQ=255)
                                tttAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCaa                                                                                                             >  3:75374/1‑150 (MQ=255)
                                     cGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGa                                                                                                        <  3:695803/150‑1 (MQ=255)
                                      gTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGAt                                                                                                       >  1:181491/1‑150 (MQ=255)
                                       ttcGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATa                                                                                                      >  2:963943/3‑150 (MQ=255)
                                          gggTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGAt                                                                                                   <  4:528330/150‑1 (MQ=255)
                                               ggTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCctc                                                                                              <  4:75374/150‑1 (MQ=255)
                                                 tgtCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGc                                                                                                                                     <  1:787577/109‑1 (MQ=255)
                                                 tgtCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCtctc                                                                                            <  4:372393/150‑1 (MQ=255)
                                                   tCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTccc                                                                                          >  3:585874/1‑150 (MQ=255)
                                                      gatgcgatgcTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCa                                                                                       >  2:249917/1‑150 (MQ=255)
                                                           gatgcTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCtctc                                                                                            <  3:350752/140‑1 (MQ=255)
                                                           gatgcTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCtctc                                                                                            >  4:350752/1‑140 (MQ=255)
                                                             tgcTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATgg                                                                                <  2:638419/150‑1 (MQ=255)
                                                               cTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCa                                                                                       >  1:651883/1‑141 (MQ=255)
                                                               cTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCa                                                                                       <  2:651883/141‑1 (MQ=255)
                                                               cTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGa                                                                              >  2:305116/1‑150 (MQ=255)
                                                                   cGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGc                                                                          <  1:410183/150‑1 (MQ=255)
                                                                   cGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTCTCACTGTTCTGCAGCTAAAACATGCCCCCTTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGc                                                                          <  3:167813/150‑1 (MQ=255)
                                                                    gCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGAtt                                                                                                  >  1:846580/1‑125 (MQ=255)
                                                                    gCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGAtt                                                                                                  <  2:846580/125‑1 (MQ=255)
                                                                    gCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCg                                                                         <  2:780708/150‑1 (MQ=255)
                                                                    gCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCg                                                                         >  2:872790/1‑150 (MQ=255)
                                                                     cATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACCAACCCCCATTCCTGGCGTATATATGGATAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGt                                                                        <  3:299082/150‑1 (MQ=255)
                                                                      aTCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTa                                                                       <  1:1227685/150‑1 (MQ=255)
                                                                       tCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAg                                                                      >  1:689185/1‑150 (MQ=255)
                                                                        cTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTACGGGAATGCGTAGt                                                                     >  2:1238174/1‑150 (MQ=255)
                                                                         ttATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATgg                                                                                >  2:281047/1‑138 (MQ=255)
                                                                          tATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAAtg                                                                                                           >  3:234529/1‑110 (MQ=255)
                                                                          tATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAAtg                                                                                                           <  4:234529/110‑1 (MQ=255)
                                                                            tCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATAATCGGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACt                                                                 >  1:319868/1‑150 (MQ=255)
                                                                              cGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGACTCCTCTCCCGCATCCATGGGACTGCCCAGTGACTTa                                                               >  4:106764/1‑150 (MQ=255)
                                                                               gCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATtc                                                                                                                  <  1:970333/98‑1 (MQ=255)
                                                                               gCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATtc                                                                                                                  >  2:970333/1‑98 (MQ=255)
                                                                                 ccTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTc                                                            >  2:765962/1‑150 (MQ=255)
                                                                                 ccTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTc                                                            <  2:1123164/150‑1 (MQ=255)
                                                                                  cTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTAt                                                                                  <  2:508496/127‑1 (MQ=255)
                                                                                  cTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTAt                                                                                  >  1:508496/1‑127 (MQ=255)
                                                                                   tACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTa                                                          >  3:357082/1‑150 (MQ=255)
                                                                                    aCCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATcccc                                                                                                                                               <  1:1293254/64‑1 (MQ=255)
                                                                                    aCCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATcccc                                                                                                                                               >  2:1293254/1‑64 (MQ=255)
                                                                                        tctctcCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAAtgtg                                                                                                         >  4:330371/1‑98 (MQ=255)
                                                                                        tctctcCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAAtgtg                                                                                                         <  3:330371/98‑1 (MQ=255)
                                                                                        tctctcCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAattat                                                     >  1:301323/1‑150 (MQ=255)
                                                                                            tcCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTAttttt                                                 >  2:34103/1‑150 (MQ=255)
                                                                                            tcCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTAttttt                                                 <  3:802645/150‑1 (MQ=255)
                                                                                            tcCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTAttttt                                                 <  1:34103/150‑1 (MQ=255)
                                                                                              ccGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTtat                                               <  1:305116/150‑1 (MQ=255)
                                                                                              ccGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTtat                                               <  4:759279/150‑1 (MQ=255)
                                                                                                    aCATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGc                                         >  4:14553/1‑150 (MQ=255)
                                                                                                        ttATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATcc                                     >  2:1181362/1‑150 (MQ=255)
                                                                                                          aTTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGt                                   >  1:46980/1‑150 (MQ=255)
                                                                                                          aTTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGt                                   <  2:733109/150‑1 (MQ=255)
                                                                                                           ttGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTg                                  >  1:837085/1‑150 (MQ=255)
                                                                                                             gCCGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGAt                                >  1:385151/1‑150 (MQ=255)
                                                                                                               cGCTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATgg                                                                                <  3:784161/100‑1 (MQ=255)
                                                                                                                gCTTTTGTTTACATATTCTGTCTCTAAACACTCCCCCTTCCCTGGCGTATATCTGGCTAACATTCAACAATGTTGTAGATTCCTCTACCGCATTTATGAAAATGCGTAGTGCCTTATTCTAATTATTTATATAATAGCATCCGTGATatt                             <  4:353328/150‑3 (MQ=255)
                                                                                                                 cTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGt                                   <  1:672578/143‑1 (MQ=255)
                                                                                                                 cTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGt                                   >  2:672578/1‑143 (MQ=255)
                                                                                                                 cTTTTGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACGTTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATg                            >  3:655798/1‑150 (MQ=255)
                                                                                                                    ttGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGaaa                         >  1:783550/1‑150 (MQ=255)
                                                                                                                     tGTTTACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGaaaa                        <  2:502662/150‑1 (MQ=255)
                                                                                                                       tttACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCCGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTTTTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAgg                      <  1:38065/150‑1 (MQ=255)
                                                                                                                        ttACATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGc                     >  3:801961/1‑150 (MQ=255)
                                                                                                                          aCATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAAt                                                                                                            >  1:195875/1‑61 (MQ=255)
                                                                                                                          aCATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAAt                                                                                                            <  2:195875/61‑1 (MQ=255)
                                                                                                                          aCATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTc                                                                                                 <  1:78555/72‑1 (MQ=255)
                                                                                                                          aCATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTc                                                                                                 >  2:78555/1‑72 (MQ=255)
                                                                                                                          aCATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTta                                                >  1:681461/1‑121 (MQ=255)
                                                                                                                          aCATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTta                                                <  2:681461/121‑1 (MQ=255)
                                                                                                                          aCATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCaa                   >  1:122984/1‑150 (MQ=255)
                                                                                                                           cATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCt                                                                                                                             <  2:629556/43‑1 (MQ=255)
                                                                                                                           cATATTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCt                                                                                                                             >  1:629556/1‑43 (MQ=255)
                                                                                                                             tatTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTa                                                               >  2:566101/1‑103 (MQ=255)
                                                                                                                             tatTCTGCCGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTa                                                               <  1:566101/103‑1 (MQ=255)
                                                                                                                                     cGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCaa        <  2:46980/150‑1 (MQ=255)
                                                                                                                                     cGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCaa        >  3:602249/1‑150 (MQ=255)
                                                                                                                                     cGCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGAATCCTTCTACTTATTTTTATAACAGCATCCGTGATAATGAAAAGGAAAAGAAACGTCaa        >  1:127667/1‑150 (MQ=255)
                                                                                                                                      gCTAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAt       <  2:747793/150‑1 (MQ=255)
                                                                                                                                        tAAACAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCctc                                                                                              >  1:23193/1‑61 (MQ=255)
                                                                                                                                          aaCAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGt   >  1:246835/1‑150 (MQ=255)
                                                                                                                                           aCAATCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGtt  >  4:104503/1‑150 (MQ=255)
                                                                                                                                             aaTCCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATa                                                                                                      >  4:385433/1‑48 (MQ=255)
                                                                                                                                               ttCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATaaa                                            <  1:685904/104‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTT  >  NC_000913_3_pae_tpiA/3853662‑3853950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: